Life Sciences Commons^™

A Comparative Electrophoretic Study Of The Triatoma Rubida Complex (Hemiptera: Reduviidae: Triatominae), Robert R. Adams

Loma Linda University Electronic Theses, Dissertations & Projects

A taxonomic study of the Triatoma Rubida Complex was conducted at the subspecific and hybrid levels using cellulose acetate electrophoresis to differentiate proteins in the hemolymph.

The following studies were conducted and results obtained: Laboratory-induced bleeding over a 4-week period markedly lowers the total protein of the bugs studied. Patterns obtained from chicken-fed and rabbit-fed bugs, although similar, showed some noticeable differences. Pathological-appearing bugs demonstrated protein patterns that varied greatly from the patterns obtained of normal bugs. The protein patterns obtained from hemolymph of 4th and 5th instar nymphs were quite similar, except for distance of migration. The computer was …

Host And Prophage Modifications Of Ultraviolet Induction In Salmonella Potsdam, Bela B. Toth

Loma Linda University Electronic Theses, Dissertations & Projects

In induction experiments with naturally occurring and artificially lysogenic strains of S. potsdam, cells were irradiated in buffer, transferred to complete medium, and then assayed for free phage. Irradiation was followed by half hourly samples for free phage from the growing populations of irradiated cells. For an estimation of burst sizes, the irradiated cells were diluted out to a final concentration of 1 cell per ml and distributed to tubes in 0.5 ml quantities. All assays were done by the top-layer method with streptomycin incorporated in the top-layer and SP2-str^r as the bacterial indicator. The non-lysogenic strain S. …

The Fecal Shedding Of Listeria Monocytogenes By Conventional And "Germ Free" Mice, Margaret Joyce Ramsay

Loma Linda University Electronic Theses, Dissertations & Projects

A series of experiments involving Listeria monocytogenes was undertaken to study first the fecal shedding of Listeria by conventional mice challenged intraperitoneally with Listeria and second the fecal shedding and ability to survive of germ free mice with similar challenge. In addition, mites infesting Listeria infected mice were cultured for their possible contamination by Listeria.

Conventional mice were challenged intraperitoneally with a single near LD₅₀ dose of Listeria. Fecal Listeria were detected by collecting specimens from each mouse daily for the first 4 days and every second day thereafter. The feces was held at 7°C for 14 …

Biochemical Properties Of Salmonella Potsdam Bacteriophage P4/2, Clyde L. Roggenkamp

Loma Linda University Electronic Theses, Dissertations & Projects

Over 90% recovery of phage P4/2 was realized with differential centrifugation concentration and purification, whereas only about 16% was recovered from the aqueous two-phase polymer - Genetron - ECTEOLA-cellulose column system. Purity of preparations was greatest with the density gradient centrifugation (A₂₆₀ = 2.66 and A₂₈₀ = 1.80 per 10¹² plaque-forming units). With the chemical system of concentration and purification followed by an ECTEOLA anion exchange run, the A₂₆₀ was 6.6 and the A₂₈₀ was 4.3.

Three tests were employed to determine the configuration of P4/2 DNA: (1) a formalin denaturation test, (2) a thermal …

Molecular Taxonomy Of Triatominae, Henry D. Brodie

Loma Linda University Electronic Theses, Dissertations & Projects

A taxonomic study of the subfamily Triatominae was conducted to determine systematic relationships using agar gel diffusion methods in the differentiation of ova antigens and electrophoresis to differentiate hernolymph proteins.

Agar gel diffusion methods indicated that two distinctive Triatominae species from South America could be distinguished from typical North American species; it was also possible to distinguish the monotypic genus Dipetalogaster from other North American populations.

A total of 180 electrophoretic tracings of Triatominae hernolyrnph were made from 18 populations of kissing bugs. Closely related populations of the Triatoma Protracta, Triatoma Rubida, and Paratriatoma Hirsuta Complexes showed group relationships within …