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Genomic And Culturomic Analysis Of Gut Microbiota Function And Salmonella Enterica Expansion, Gavin Fenske
Genomic And Culturomic Analysis Of Gut Microbiota Function And Salmonella Enterica Expansion, Gavin Fenske
Electronic Theses and Dissertations
Enteric bacteria that are resident in the hindgut of mammals are critical in immune development, digestion, and colonization resistance against pathogens. One of the major pathogens that gut commensals provide resistance against is Salmonella enterica, a major foodborne pathogen capable of infecting almost every warm-blooded animal. Given the interplay between pathogens and commensals in the gut lumen, the gut microbiota of pigs was studied by combining two disparate techniques: shotgun metagenomics and high throughput culturomics. Metagenomics readily identifies major taxa present in samples and can give an estimation to total genetic catalogue from an environment. However, many rare or …
Detection Of Bacterial Retroelements Using Genomics, Sen Mu
Detection Of Bacterial Retroelements Using Genomics, Sen Mu
Electronic Theses and Dissertations
The reverse flow of genetic information can occur when a special DNA polymerase called Reverse Transcriptase (RT) copies the genetic information in an RNA molecule back into a complementary DNA. One type of RT encoding gene found in bacteria is called a retron element. Recent bacterial genome sequencing projects have revealed many examples of retron RT genes. This gene assignment is based on comparison with a few known retron RT proteins. However, RT proteins are highly diverse in their amino acid sequences, and thus the assigned identity of these RT proteins as retrons in genome databases is questionable. One way …