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Full-Text Articles in Life Sciences
The Role Of A-Layer In Polyunsaturated Fatty Acid (Pufa)-Mediated Effects On Aeromonas Salmonicida Subsp. Salmonicida, Allen Lin
Honors Theses
Aeromonas salmonicida subsp. salmonicida is a Gram-negative bacterium that infects salmonids and non-salmonids worldwide leading to an infection known as furunculosis, which is characterized by skin lesions and hemorrhages of the fish epidermis. This infection is carried out by a A+ (virulent strain) of A. salmonicida containing an important virulent factor known as the A-layer, which is a 2D paracrystalline structure that binds to the basement membrane and functions to promote adherence to host membranes and resistance to host defense. The purpose of this study was to examine the ability of the A+ A. salmonicida to incorporate exogenous fatty acids …
Calibration Study Of A Bacterial Quantification Technique Using Transport Swabs And Line Inoculations On Agar Growth Medium, Seth Larue
Honors Theses
Enumeration of bacteria has been the subject of research for over a century. Some of the techniques that have been developed include viable plate counts, the most probable number assessment, and more modern molecular techniques. Many of the newer molecular-based techniques provide little or no information about the viability of the cells being counted, and are generally not quantitative. Having quantitative data for potentially pathogenic bacteria on surfaces can be very useful in many settings, particularly in healthcare facilities. Knowing the numbers of bacterial cells present on surfaces within healthcare facilities may be important to attempt to study the cause …
A Metabolomics-Based Approach To The Screening Of Endometrial Cancer: Development Of A Gas Chromatography-Ion Trap Mass Spectrometry-Based Method, Allycia Lee
Honors Theses
Endometrial Cancer (EC) is the most common malignant tumor of the female reproductive tract. Currently there are no methods for effectively screening EC, but one method that is thought to be useful in screening EC is metabolomics. Metabolomics involves the analysis of metabolites which are low molecular weight inorganic and organic chemicals that are substrates, intermediates, and by-products of enzyme-mediated biochemical reactions in the cell. Previous research shows that the metabolic signature of EC patients are discernable from those of healthy patients. To aid in the development of an effective screening method for EC, blood serum, urine, and saliva samples …
Increasing Stress Tolerance In Saccharomyces Cerevisiae Using Lipocalin Genes, Ximena Leon
Increasing Stress Tolerance In Saccharomyces Cerevisiae Using Lipocalin Genes, Ximena Leon
Honors Theses
Saccharomyces cerevisiae is a microorganism that is commonly used in industries, but its productivity is significantly hindered by its inability to resist high stress levels found in industrial processes. By increasing stress tolerance in S. cerevisiae, its role in industrial processes could be maximized. The objective of this study is to determine if complementation of lipocalin genes into the wildtype and knockout strains of S. cerevisiae can increase stress tolerance against a variety of stressors. We identified the knockout yeast strains, ALD3, ALD4, PDX3, and ILV1, as being sensitive to various stressors including salinity, heat, oxidative, and osmotic stresses. Arabidopsis …