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Microbiology

University of Tennessee, Knoxville

Masters Theses

Theses/Dissertations

2004

Articles 1 - 3 of 3

Full-Text Articles in Life Sciences

Sol-Gel Encapsulation Of A Bioluminescent Bioreporter, Elizabeth F. Mitchell Aug 2004

Sol-Gel Encapsulation Of A Bioluminescent Bioreporter, Elizabeth F. Mitchell

Masters Theses

Genetic engineering has allowed for the development of a number of whole-cell bacterial bioreporters. In order for these cells to be used in certain devices and field applications, they must be maintained, and protected, but exposed to environmental conditions. One approach used to accomplish this goal is cellular encapsulation. The recent development of techniques to form inorganic matrices, in particular through the sol-gel process, displays promise as a system of encapsulation. The silica sol-gel process was originally designed for the fabrication of glass, and given that reactions are performed in standard lab conditions and room temperature, the process can be …


Identifying The Signature Of The Natural Attenuation Of Mtbe In Groundwater Using Molecular Methods And "Bug Traps", Anita Eva Biernacki Aug 2004

Identifying The Signature Of The Natural Attenuation Of Mtbe In Groundwater Using Molecular Methods And "Bug Traps", Anita Eva Biernacki

Masters Theses

Natural attenuation through intrinsic bioremediation is the risk-based management approach commonly used for gasoline (BTEX) contamination sites. This approach has not yet been utilized for the fuel oxygenate methyl tertiary-butyl ether (MTBE). MTBE is more resistant to biodegradation than BTEX. MTBE is more abundant than benzene in oxygenated gasoline, has a greater water solubility than BTEX, and sorbs weakly to soil. These properties complicate developing a risk based management option to be implemented as easily as for BTEX. The purpose of this project is to contribute to a growing database containing information on MTBE contaminated sites nationwide with a variety …


The Effects Of Iron On Growth And Physiology Of The Cyanobacterium Microcystis Aeruginosa, Shannon Pedigo Efteland May 2004

The Effects Of Iron On Growth And Physiology Of The Cyanobacterium Microcystis Aeruginosa, Shannon Pedigo Efteland

Masters Theses

To determine the effects of iron on the growth of the cyanobacterium Microcystis aeruginosa, a series of growth and iron uptake experiments were conducted. Studies included the effects of iron and organic iron-binding chelators on batch cultures of known bacteria and on the natural community of Lake Erie, where blooms of M. aeruginosa have been documented since 1995. Results of growth rate studies under iron limitation suggest that M. aeruginosa uses an active, high-affinity transport system to acquire iron after a lag time at initial limitation. Further studies indicate that cells continue to take up nutrients and possibly metals, while …