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Full-Text Articles in Life Sciences
The Generation Of Influenza-Specific Humoral Responses Is Impaired In St6gal I-Deficient Mice., Thandi M. Onami, J Zeng, H. M. Joo, B. Rajini, J. P. Wrammert, M. Y. Sangster
The Generation Of Influenza-Specific Humoral Responses Is Impaired In St6gal I-Deficient Mice., Thandi M. Onami, J Zeng, H. M. Joo, B. Rajini, J. P. Wrammert, M. Y. Sangster
Thandi M. Onami
Posttranslational modification of proteins, such as glycosylation, can impact cell signaling and function. ST6Gal I, a glycosyltransferase expressed by B cells, catalyzes the addition of alpha-2,6 sialic acid to galactose, a modification found on N-linked glycoproteins such as CD22, a negative regulator of B cell activation. We show that SNA lectin, which binds alpha-2,6 sialic acid linked to galactose, shows high binding on plasma blasts and germinal center B cells following viral infection, suggesting ST6Gal I expression remains high on activated B cells in vivo. To understand the relevance of this modification on the antiviral B cell immune response, we …
Role Of Chromodomain Helicase Dna-Binding Protein 2 In Dna Damage Response Signaling And Tumorigenesis., Thandi M. Onami, P. Nagarajan, S. Rajagopalan, S. Kania, R. Donnell, S. Venkatachalam
Role Of Chromodomain Helicase Dna-Binding Protein 2 In Dna Damage Response Signaling And Tumorigenesis., Thandi M. Onami, P. Nagarajan, S. Rajagopalan, S. Kania, R. Donnell, S. Venkatachalam
Thandi M. Onami
The chromodomain helicase DNA-binding proteins (CHDs) are known to affect transcription through their ability to remodel chromatin and modulate histone deacetylation. In an effort to understand the functional role of the CHD2 in mammals, we have generated a Chd2 mutant mouse model. Remarkably, the Chd2 protein appears to play a critical role in the development, hematopoiesis and tumor suppression. The Chd2 heterozygous mutant mice exhibit increased extramedullary hematopoiesis and susceptibility to lymphomas. At the cellular level, Chd2 mutants are defective in hematopoietic stem cell differentiation, accumulate higher levels of the chromatin-associated DNA damage response mediator, cH2AX, and exhibit an aberrant …