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Desiccation Induces Viable But Non-Culturable Cells In Sinorhizobium Meliloti 1021, Jan A.C. Vriezen Dr., Frans J. Debruijn Dr., Klaus R. Nusslein Dr.
Desiccation Induces Viable But Non-Culturable Cells In Sinorhizobium Meliloti 1021, Jan A.C. Vriezen Dr., Frans J. Debruijn Dr., Klaus R. Nusslein Dr.
Jan A.C. Vriezen Dr.
Sinorhizobium meliloti is a microorganism commercially used in the production of e.g. Medicago sativa seed inocula. Many inocula are powder-based and production includes a drying step. Although S. meliloti survives drying well, the quality of the inocula is reduced during this process. In this study we determined survival during desiccation of the commercial strains 102F84 and 102F85 as well as the model strain USDA1021. The survival of S. meliloti 1021 was estimated during nine weeks at 22% relative humidity. We found that after an initial rapid decline of colony forming units, the decline slowed to a steady 10-fold reduction in …
Responses Of Rhizobia To Desiccation In Relation To Osmotic Stress, Oxygen, And Temperature, Jan A.C. Vriezen Dr., Frans J. Debruijn Dr., Klaus R. Nusslein Dr.
Responses Of Rhizobia To Desiccation In Relation To Osmotic Stress, Oxygen, And Temperature, Jan A.C. Vriezen Dr., Frans J. Debruijn Dr., Klaus R. Nusslein Dr.
Jan A.C. Vriezen Dr.
No abstract provided.
Desiccation Responses And Survival Of Sinorhizobium Meliloti Usda 1021 In Relation To Growth Phase, Temperature, Chloride And Sulfate Availability, Jan A.C. Vriezen Dr., Frans J. Debruijn Dr., Klaus R. Nusslein Dr.
Desiccation Responses And Survival Of Sinorhizobium Meliloti Usda 1021 In Relation To Growth Phase, Temperature, Chloride And Sulfate Availability, Jan A.C. Vriezen Dr., Frans J. Debruijn Dr., Klaus R. Nusslein Dr.
Jan A.C. Vriezen Dr.
Aims: To identify physical and physiological conditions that affect the survival of Sinorhizobium meliloti USDA 1021 during desiccation. Methods and Results: An assay was developed to study desiccation response of S. meliloti USDA 1021 over a range of environmental conditions. We deter- mined the survival during desiccation in relation to (i) matrices and media, (ii) growth phase, (iii) temperature, and (iv) chloride and sulfate availability. Conclusions: This study indicates that survival of S. meliloti USDA 1021 during desiccation is enhanced: (i) when cells were dried in the stationary phase, (ii) with increasing drying temperature at an optimum of 37°C, and …
A New Genetic Locus In Sinorhizobium Meliloti Is Involved In Stachydrine Utilization, Donald A. Phillips Dr., Eve S. Sandee Dr., Jan A.C. Vriezen Dr., Frans J. Debruijn Dr., Daniel Lerudulier Dr., Cecillia M. Joseph Dr.
A New Genetic Locus In Sinorhizobium Meliloti Is Involved In Stachydrine Utilization, Donald A. Phillips Dr., Eve S. Sandee Dr., Jan A.C. Vriezen Dr., Frans J. Debruijn Dr., Daniel Lerudulier Dr., Cecillia M. Joseph Dr.
Jan A.C. Vriezen Dr.
Stachydrine, a betaine released by germinating alfalfa seeds, functions as an inducer of nodulation genes, a catabolite, and an osmoprotectant in Sinorhizobium meliloti. Two stachydrine-inducible genes were found in S. meliloti 1021 by mutation with a Tn5-luxAB promoter probe. Both mutant strains (S10 and S11) formed effec- tive alfalfa root nodules, but neither grew on stachydrine as the sole carbon and nitrogen source. When grown in the absence or presence of salt stress, S10 and S11 took up [14C]stachydrine as well as wild-type cells did, but neither used stachydrine effectively as an osmoprotectant. In the absence of salt stress, both …