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Microbiology

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Microbiology

Articles 1 - 4 of 4

Full-Text Articles in Life Sciences

Nitrogen Fertilization Has A Stronger Effect On Soil Nitrogen-Fixing Bacterial Communities Than Elevated Atmospheric Co2, Sean Berthrong, Chris Yeager, Laverne Gallegos-Graves, Blaire Steven, Stephanie Eichorst, Robert Jackson, Cheryl Kuske Feb 2016

Nitrogen Fertilization Has A Stronger Effect On Soil Nitrogen-Fixing Bacterial Communities Than Elevated Atmospheric Co2, Sean Berthrong, Chris Yeager, Laverne Gallegos-Graves, Blaire Steven, Stephanie Eichorst, Robert Jackson, Cheryl Kuske

Sean Berthrong

Biological nitrogen fixation is the primary supply of N to most ecosystems, yet there is considerable uncertainty about how N-fixing bacteria will respond to global change factors such as increasing atmospheric CO2 and N deposition. Using the nifH gene as a molecular marker, we studied how the community structure of N-fixing soil bacteria from temperate pine, aspen, and sweet gum stands and a brackish tidal marsh responded to multiyear elevated CO2 conditions. We also examined how N availability, specifically, N fertilization, interacted with elevated CO2 to affect these communities in the temperate pine forest. Based on data from Sanger sequencing …


Microbial Isolations From Olive Ridley (Lepidochelys Olivacea) And Eastern Pacific Green (Chelonia Mydas Agassizii) Sea Turtle Nests In Guanacaste, Costa Rica, And Standard Testing Of Cloacal Fluid Antimicrobial Properties, Erin Keene, Tanya Soule, Frank Paladino Jun 2014

Microbial Isolations From Olive Ridley (Lepidochelys Olivacea) And Eastern Pacific Green (Chelonia Mydas Agassizii) Sea Turtle Nests In Guanacaste, Costa Rica, And Standard Testing Of Cloacal Fluid Antimicrobial Properties, Erin Keene, Tanya Soule, Frank Paladino

Tanya Soule

Microorganisms associated with olive ridley and East Pacific green turtle nesting and potential cloacal fluid antimicrobial properties were studied in Guanacaste, Costa Rica. During the 2010–2011 season, bacteria and fungi were isolated from olive ridley cloacal fluid, nest chamber sand, and egg samples. Because of the lack of cloacal fluid bacteria isolated, the focus of the 2011–2012 season shifted to determine whether fluid contained antibacterial properties by using Kirby–Bauer disk diffusion assays, and cloacal fluid and sand samples were taken to see whether bacteria were unique to cloacal fluid. Assays were performed on 34 olive ridley and 5 East Pacific …


Recombinant Expression Of Indolicidin Concatemers In Escherichia Coli, K Morin, S Arcidiacono, Richard Beckwitt, C Mello Feb 2013

Recombinant Expression Of Indolicidin Concatemers In Escherichia Coli, K Morin, S Arcidiacono, Richard Beckwitt, C Mello

Richard D Beckwitt

Antimicrobial peptides are part of the innate immune system of vertebrates and invertebrates. They are active against gram-negative and gram-positive bacteria, fungi, and protozoa. Currently, most antimicrobial peptides are extracted from host organisms or produced by solidphase peptide synthesis. Recombinant protein expression in Escherichia coli is a tool for greater production yields at a decreased cost and reduces the use of hazardous materials. We have constructed a concatamer of indolicidin and successfully expressed a fusion product with thioredoxin in E. coli BL21DE3. Codons for methionine residues flanking individual indolicidin genes were incorporated for cyanogen bromide cleavage of the fusion protein …


Survey Of Antibiotic Resistant Bacteria In Fish Gut Collected From Selected Sites., Ahmed Mustafa, Hasina Karki, Arlis Lamaster, Bob Gillespie, Shree Dhawale Dec 2011

Survey Of Antibiotic Resistant Bacteria In Fish Gut Collected From Selected Sites., Ahmed Mustafa, Hasina Karki, Arlis Lamaster, Bob Gillespie, Shree Dhawale

Ahmed Mustafa Dr.

No abstract provided.