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T-Rfpred: A Nucleotide Sequence Size Prediction Tool For Microbial Community Description Based On Terminal-Restriction Fragment Length Polymorphism Chromatograms, Antonio Fernandez-Guerra, Alison Buchan, Xiaozhen Mou, Emilio O. Casamayor, Jose M. Gonzalez
T-Rfpred: A Nucleotide Sequence Size Prediction Tool For Microbial Community Description Based On Terminal-Restriction Fragment Length Polymorphism Chromatograms, Antonio Fernandez-Guerra, Alison Buchan, Xiaozhen Mou, Emilio O. Casamayor, Jose M. Gonzalez
Xiaozhen Jen Mou
Background
Terminal-Restriction Fragment Length Polymorphism (T-RFLP) is a technique used to analyze complex microbial communities. It allows for the quantification of unique or numerically dominant phylotypes in amplicon pools and it has been used primarily for comparisons between different communities. T-RFPred, Terminal-Restriction Fragment Prediction, was developed to identify and assign taxonomic information to chromatogram peaks of a T-RFLP fingerprint for a more comprehensive description of microbial communities. The program estimates the expected fragment size of representative 16S rRNA gene sequences (either from a complementary clone library or from public databases) for a given primer and restriction enzyme(s) and provides candidate …