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Full-Text Articles in Life Sciences
Influence Of The Hydrodynamic Environment On Quorum Sensing In Pseudomonas Aeruginosa Biofilms, Jeffrey J. Margolis, Mary Jo Kirisits, Boloroo L. Purevdorj-Gage, Benjamin Vaughan, David L. Chopp, Paul Stoodley, Matthew R. Parsek
Influence Of The Hydrodynamic Environment On Quorum Sensing In Pseudomonas Aeruginosa Biofilms, Jeffrey J. Margolis, Mary Jo Kirisits, Boloroo L. Purevdorj-Gage, Benjamin Vaughan, David L. Chopp, Paul Stoodley, Matthew R. Parsek
Jeffrey Margolis
We provide experimental and modeling evidence that the hydrodynamic environment can impact quorum
sensing (QS) in a <em>Pseudomonas aeruginosa</em> biofilm. The amount of biofilm biomass required for full QS induction of the population increased as the flow rate increased.
In Vivo Negative Selection Screen Identifies Genes Required For Francisella Virulence, David S. Weiss, Anna Brotcke, Thomas Henry, Jeffrey J. Margolis, Kaman Chan, Denise M. Monack
In Vivo Negative Selection Screen Identifies Genes Required For Francisella Virulence, David S. Weiss, Anna Brotcke, Thomas Henry, Jeffrey J. Margolis, Kaman Chan, Denise M. Monack
Jeffrey Margolis
<em>Francisella tularensis</em> subverts the immune system to rapidly grow within mammalian hosts, often causing tularemia, a fatal disease. This pathogen targets the cytosol of macrophages where it replicates by using the genes encoded in the <em>Francisella</em> pathogenicity island. However, the bacteria are recognized in the cytosol by the host’s ASC/caspase-1 pathway, which is essential for host defense, and leads to macrophage cell death and proinflammatory cytokine production. We used a microarray-based negative selection screen to identify <em>Francisella</em> genes that contribute to growth and/or survival in mice. The screen identified many known virulence factors including all of the Francisella pathogenicity island …