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The Effect Of Ergosterol On The Desaturation Of 14c-Labeled Oleic Acid In Tetrahymena, Maynard E. Neville Jr.
The Effect Of Ergosterol On The Desaturation Of 14c-Labeled Oleic Acid In Tetrahymena, Maynard E. Neville Jr.
Masters Theses
The ciliated protozoan Tetrahymena pyriformis-Strain W desaturated 14C-labeled fatty acid substrate in both sterol supplemented (ergosterol) and nonsupplemented cultures. Cis-octadecenoic acid (14C-9-18:1), when added to the medium during the log phase of growth, was incorporated into the cells and also desaturated at the Δ6 and Δ12 positions. The 14C labeled substrate and products, linoleate (9,12-18:2) or linolenate (6,9,12-18:3) were recovered and separated by silver-nitrate Unisil column chromatography. Initially, recovery of 14C compounds varied widely when expressed as the percent of total counts per minute recovered from column chromotography, and a variety of …