Life Sciences Commons^™

Effects Of Conalbumin Bound Iron On The Growth Of Salmonella Paratyphi B And Salmonella Thompson, John Nicholas Mason

Dissertations and Theses

I have investigated the possibility that specific conalbumin (ovotransferrin) iron saturation levels enable less virulent strains of Salmonella to become more virulent. Iron starved cells of two pathogenic Salmonella strains, S. paratyphi B var. java and S. thompson, were cultured in iron limited media at 3 different iron conalbumin saturation levels. Results indicate that strains differ significantly at both low and high iron saturation conalbumin. These differences depict a growth advantage for S. paratyphi B which correlates with reports by the Centers for Disease Control that S. paratyphi B was 3 times more frequent in blood isolates than S. …

Aerotaxis In Halobacterium Halobium, Jemima Clara Lindbeck

Loma Linda University Electronic Theses, Dissertations & Projects

Aerotaxis, previously studied in eubacteria was investigated in the archaebacterium Halobacterium halobium. A quantitative temporal assay for aerotaxis of H. halobium was developed using computer assisted motion analysis. Aerotaxis was most pronounced in early log phase cultures. Peak aerotaxis was found to coincide with peak respiration consistent with a model in which respiration is required for aerotaxis. In a spatial assay a mutant strain lacking the proton motive force (pmf)-generating pigments, bacteriorhodopsin (bR) and halorhodopsin (hR), exhibited enhanced aerotaxis bands relative to the bR⁺hR⁺ parent. The enhanced aerotaxis in the mutant cells was not due solely to …

A Flow Cytometric Analysis Of Immunocyte Populations In Peripheral Blood And Pleural Effusions Of Patients With Cancer, Mandy L. Bohn

Loma Linda University Electronic Theses, Dissertations & Projects

Two-color flow cytometric analysis was performed on paired samples of peripheral blood (PB) and pleural effusions (PE) of patients with metastatic malignancies. The purpose of these analyses was to test the hypothesis that there are significant differences in the distribution of immunocyte populations in the PE compared to that of the PB.

The majority of the pleural fluid immunocytes were CD3+ cells. The ratio of CD4+ to CD8+ cells was higher in the PE compared to PB. Levels of CD8+, CD19+ and CD14+ cells were not significantly different in the PE compared to the levels in the PB. CD16+CD56+ cells …

Use Of Antisense Rna In The Study Of Essential Cell Division Genes In Escherichia Coli, Quynh V. Nguyen

Honors Theses

No abstract provided.

The Study Of Natural Product(S) From Opsanus Tau (Linnaeus), The Oyster Toadfish, Miki Taira

Chemistry & Biochemistry Theses & Dissertations

Opsanus tau (Linnaeus), the Oyster toadfish, commonly inhabits the Chesapeake Bay. The Oyster toadfish has been categorized as a venomous and/or ichthyotoxic fish; however, chemical studies to substantiate these claims have not been forthcoming.

The oyster toadfish has dorsal and opercular spines which are believed to secrete a venom(s). Based on our findings, the oyster toadfish does not secrete venom(s) from these spines.

The oyster toadfish secretes large amounts of a mucus when it is irritated. 3-Octanone was believed to be the major ichthyotoxic component of the mucus from the oyster toadfish. Based on our investigation, 3-octanone is present only …

Quantitation Of Type I Collagen Mrna In Intact Human Fibroblasts, Edward Touma

Master's Theses

No abstract provided.

Interleukin-2 Activated Anti-Fungal Activity Of Lymphocytes, David W.A. Beno

Dissertations

No abstract provided.

Reactivity Of Anti-Group A Type 12 Streptococcal Cell Membrane Monoclonal Antibody To Human Glomerular Basement Membrane, Sally C. Kent

Dissertations

No abstract provided.

Isolation And Immunochemical Characterization Of Streptococcal Cell Membrane Antigens Immunologically Related To Basement Membrane, Mark E. Zelman

Dissertations

No abstract provided.

Direct Immunofluorescence Detection Of Giardia Spp. In Selected Mammals In Central Illinois, Using Anti-Giardia Polyclonal And Monoclonal Antibodies, Jeanette Aba Ackon Brown

Masters Theses

Giardia species occur in many kinds of mammals, and some of these hosts have been postulated as potential reservoirs for human infections. A study was initiated in fall 1990 to determine the distribution and frequency of giardiasis in populations of selected wild animals in 12 counties in Illinois. Fecal samples from 64 white-tailed deer, 13 coyotes, 9 muskrats, 6 raccoons, and 5 badgers were examined for the presence of Giardia spp. and specifically for G. lamblia. Anti-Giardia lamblia cysts polyclonal (PAb) and monoclonal (MAb), FITC-labeled antibody solutions were used for the direct immunofluorescence detection of Giardia cysts in …