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Isolation And Partial Characterization Of A Novel Lectin From Aegle Marmelos Fruit And Its Effect On Adherence And Invasion Of Shigella To Ht29 Cells, Raja Sb, Murali Mr, Kumar Nk, Devaraj Sn
Isolation And Partial Characterization Of A Novel Lectin From Aegle Marmelos Fruit And Its Effect On Adherence And Invasion Of Shigella To Ht29 Cells, Raja Sb, Murali Mr, Kumar Nk, Devaraj Sn
Murali Malliga Raman
Lectins are a class of ubiquitous proteins/glycoproteins that are abundantly found in nature. Lectins have unique carbohydrate binding property and hence have been exploited as drugs against various infectious diseases. We have isolated one such novel lectin from the fruit pulp of Aegle marmelos. The isolated lectin was partially characterised and its effect against Shigella dysenteriae infection was evaluated. The isolated lectin was found to be a dimeric protein with N-acetylgalactosamine, mannose and sialic acid binding specificity. The effect of Aegle marmelos fruit lectin on the adherence of Shigella dysenteriae to human colonic epithelial cells (HT29 cells) was evaluated by …
Lactobacilli Inhibit Shigella Dysenteriae 1 Induced Pro-Inflammatory Response And Cytotoxicity In Host Cells Via Impediment Of Shigella-Host Interactions, Murali Mr, Moorthy G, Niranjali Devaraj S
Lactobacilli Inhibit Shigella Dysenteriae 1 Induced Pro-Inflammatory Response And Cytotoxicity In Host Cells Via Impediment Of Shigella-Host Interactions, Murali Mr, Moorthy G, Niranjali Devaraj S
Murali Malliga Raman
OBJECTIVE: Shigella dysenteriae Type 1 dysentery is a major cause of morbidity and mortality in children from less developed and developing countries. The present study explores the hypothesis that lactobacilli protect the host cell during S. dysenteriae Type 1 infection and its mechanism of action. METHODS: Caco-2 cells incubated for 1h with Lactobacillus rhamnosus or Lactobacillus acidophilus at the multiplicity of infection of 100, either alone or in combination followed by addition of Shigella at the same multiplicity of infection for 5h served as treatment groups. Cells incubated with Shigella without lactobacilli addition served as infected cells. At the end …
Effect Of Aqueous Extract Of Aegle Marmelos Fruit On Adherence And Β-Lactam Resistance Of Enteropathogenic Escherichia Coli By Down Regulating Outer Membrane Protein C, Murali Malliga Raman
Effect Of Aqueous Extract Of Aegle Marmelos Fruit On Adherence And Β-Lactam Resistance Of Enteropathogenic Escherichia Coli By Down Regulating Outer Membrane Protein C, Murali Malliga Raman
Murali Malliga Raman
Problem statement: Enteropathogenic Escherichia Coli (EPEC) continue to be a major health problem, leading to death due to diarrhea, predominantly in children below the age of five. Due to evolution of multi drug resistance in EPEC and side effects caused to host by antibiotics necessitated a search for alternative medicines from medicinal plants. One such medicinal plant used since ancient times to cure diarrhea is Aegle marmelos. This study was done to investigate the effect of aqueous extract of Aegle marmelos fruit (AEAM) on outer membrane protein C (OmpC) of EPEC, which plays a key role in adherence and antibiotic …