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Full-Text Articles in Life Sciences
Francisella Tularensis Type A Strains Cause The Rapid Encystment Of Acanthamoeba Castellanii And Survive In Amoebal Cysts For Three Weeks Postinfection, Jeffrey J. Margolis, Sahar H. El-Etr, Denise Monack, Richard A. Robinson, Marissa Cohen, Emily Moore, Amy Rasley
Francisella Tularensis Type A Strains Cause The Rapid Encystment Of Acanthamoeba Castellanii And Survive In Amoebal Cysts For Three Weeks Postinfection, Jeffrey J. Margolis, Sahar H. El-Etr, Denise Monack, Richard A. Robinson, Marissa Cohen, Emily Moore, Amy Rasley
Jeffrey Margolis
Archaeal Populations In Biological Soil Crusts From Arid Lands In North America, Tanya Soule, Ian J. Anderson, Shannon L. Johnson, Scott T. Bates, Ferran Garcia-Pichel
Archaeal Populations In Biological Soil Crusts From Arid Lands In North America, Tanya Soule, Ian J. Anderson, Shannon L. Johnson, Scott T. Bates, Ferran Garcia-Pichel
Tanya Soule
Archaea are common and abundant members of biological soil crust communities across large-scale biogeographic provinces of arid North America. Regardless of microbial community development, archaeal populations averaged 2 × 107 16S rRNA gene copies per gram of soil, representing around 5% of the prokaryotic (total calculated bacterial and archaeal) numbers assessed by quantitative-PCR. In contrast, archaeal diversity, determined by denaturing gradient gel electrophoresis fingerprinting and clone libraries of 16S rRNA genes, was very restricted. Only six different phylotypes (all Crenarchaea) were detected, three of which were very dominant. Some phylotypes were widespread, while others were typical of Southern desert areas.
A Comparative Genomics Approach To Understanding The Biosynthesis Of The Sunscreen Scytonemin In Cyanobacteria, Tanya Soule, Kendra Palmer, Qunjie Gao, Ruth M. Potrafka, Valerie Stout, Ferran Garcia-Pichel
A Comparative Genomics Approach To Understanding The Biosynthesis Of The Sunscreen Scytonemin In Cyanobacteria, Tanya Soule, Kendra Palmer, Qunjie Gao, Ruth M. Potrafka, Valerie Stout, Ferran Garcia-Pichel
Tanya Soule
Background The extracellular sunscreen scytonemin is the most common and widespread indole-alkaloid among cyanobacteria. Previous research using the cyanobacterium Nostoc punctiforme ATCC 29133 revealed a unique 18-gene cluster (NpR1276 to NpR1259 in the N. punctiforme genome) involved in the biosynthesis of scytonemin. We provide further genomic characterization of these genes in N. punctiforme and extend it to homologous regions in other cyanobacteria.
Results Six putative genes in the scytonemin gene cluster (NpR1276 to NpR1271 in the N. punctiforme genome), with no previously known protein function and annotated in this study as scyA to scyF, are likely involved in the assembly …
Gene Expression Patterns Associated With The Biosynthesis Of The Sunscreen Scytonemin In Nostoc Punctiforme Atcc 29133 In Response To Uva Radiation, Tanya Soule, Ferran Garcia-Pichel, Valerie Stout
Gene Expression Patterns Associated With The Biosynthesis Of The Sunscreen Scytonemin In Nostoc Punctiforme Atcc 29133 In Response To Uva Radiation, Tanya Soule, Ferran Garcia-Pichel, Valerie Stout
Tanya Soule
Under exposure to UV radiation, some cyanobacteria synthesize sunscreen compounds. Scytonemin is a heterocyclic indole-alkaloid sunscreen, the synthesis of which is induced upon exposure to UVA (long-wavelength UV) radiation. We previously identified and characterized an 18-gene cluster associated with scytonemin biosynthesis in the cyanobacterium Nostoc punctiforme ATCC 29133; we now report on the expression response of these genes to a step-up shift in UVA exposure. Using quantitative PCR on cDNAs from the N. punctiforme transcriptome and primers targeting each of the 18 genes in the cluster, we followed their differential expression in parallel subcultures incubated with and without UVA. All …
Genome-Scale Comparison And Constraint-Based Metabolic Reconstruction Of The Facilitative Anaerobic Fe(Iii)-Reducer Rhodoferax Ferrireducens, Derek Lovley, Carla Risso, Jun Sun, Kai Zhuang, Radhakrishnan Mahadevan, Robert Deboy, Wael Ismail, Susmita Shrivastava, Heather Huot, Sagar Kothari, Sean Daughtry, Olivia Bui, Christophe Schilling, Barbara Methѐ
Genome-Scale Comparison And Constraint-Based Metabolic Reconstruction Of The Facilitative Anaerobic Fe(Iii)-Reducer Rhodoferax Ferrireducens, Derek Lovley, Carla Risso, Jun Sun, Kai Zhuang, Radhakrishnan Mahadevan, Robert Deboy, Wael Ismail, Susmita Shrivastava, Heather Huot, Sagar Kothari, Sean Daughtry, Olivia Bui, Christophe Schilling, Barbara Methѐ
Carla Risso
Background Rhodoferax ferrireducens is a metabolically versatile, Fe(III)-reducing, subsurface microorganism that is likely to play an important role in the carbon and metal cycles in the subsurface. It also has the unique ability to convert sugars to electricity, oxidizing the sugars to carbon dioxide with quantitative electron transfer to graphite electrodes in microbial fuel cells. In order to expand our limited knowledge about R. ferrireducens, the complete genome sequence of this organism was further annotated and then the physiology of R. ferrireducens was investigated with a constraint-based, genome-scale in silico metabolic model and laboratory studies. Results The iterative modeling and …
Contributions Of Gut Bacteria To Bacillus Thuringiensis-Induced Mortality Vary Across A Range Of Lepidoptera, Nichole A. Broderick, Courtney Jaime Robinson, Matthew D. Mcmahon, Kenneth F. Raffa
Contributions Of Gut Bacteria To Bacillus Thuringiensis-Induced Mortality Vary Across A Range Of Lepidoptera, Nichole A. Broderick, Courtney Jaime Robinson, Matthew D. Mcmahon, Kenneth F. Raffa
Courtney Robinson
Tracking Bacterial Responses To Global Warming With An Ecotype-Based Systematics, Frederick M. Cohan
Tracking Bacterial Responses To Global Warming With An Ecotype-Based Systematics, Frederick M. Cohan
Frederick M. Cohan
No abstract provided.
The Genome Sequence Of Geobacter Metallireducens: Features Of Metabolism, Physiology And Regulation Common And Dissimilar To Geobacter Sulfurreducens, Derek Lovley, Muktak Aklujkar, Julia Krushkal, Genevieve Dibartolo, Alla Lapidus, Miriam L. Land
The Genome Sequence Of Geobacter Metallireducens: Features Of Metabolism, Physiology And Regulation Common And Dissimilar To Geobacter Sulfurreducens, Derek Lovley, Muktak Aklujkar, Julia Krushkal, Genevieve Dibartolo, Alla Lapidus, Miriam L. Land
Muktak Aklujkar
BACKGROUND: The genome sequence of Geobacter metallireducens is the second to be completed from the metal-respiring genus Geobacter, and is compared in this report to that of Geobacter sulfurreducens in order to understand their metabolic, physiological and regulatory similarities and differences. RESULTS: The experimentally observed greater metabolic versatility of G. metallireducens versus G. sulfurreducens is borne out by the presence of more numerous genes for metabolism of organic acids including acetate, propionate, and pyruvate. Although G. metallireducens lacks a dicarboxylic acid transporter, it has acquired a second putative succinate dehydrogenase/fumarate reductase complex, suggesting that respiration of fumarate was important until …
Impact Of An External Energy On Enterococcus Faecalis [Atcc – 51299] In Relation To Antibiotic Susceptibility And Biochemical Reactions – An Experimental Study, Mahendra Kumar Trivedi, Dr. Yogi Bhardwa, Shrikant Patil, Harish Shettigar, Archana Bulbule
Impact Of An External Energy On Enterococcus Faecalis [Atcc – 51299] In Relation To Antibiotic Susceptibility And Biochemical Reactions – An Experimental Study, Mahendra Kumar Trivedi, Dr. Yogi Bhardwa, Shrikant Patil, Harish Shettigar, Archana Bulbule
Mahendra Kumar Trivedi