Life Sciences Commons^™

Impact Of An External Energy On Staphylococcus Epidermis [Atcc –13518] In Relation To Antibiotic Susceptibility And Biochemical Reactions – An Experimental Study, Mahendra Kumar Trivedi, Shrikant Patil

Mahendra Kumar Trivedi

Highly Conserved Genes In Geobacter Species With Expression Patterns Indicative Of Acetate Limitation, Derek Lovley, Carla Risso, Barbara A. Methé, Hila Elifantz, Dawn E. Holmes

Carla Risso

Analysis of the genome of Geobacter sulfurreducens revealed four genes encoding putative symporters with homology to ActP, an acetate transporter in Escherichia coli. Three of these genes, aplA, aplB and aplC, are highly similar (over 90 % identical) and fell within a tight phylogenetic cluster (Group I) consisting entirely of Geobacter homologues. Transcript levels for all three genes increased in response to acetate limitation. The fourth gene, aplD, is phylogenetically distinct (Group II) and its expression was not influenced by acetate availability. Deletion of any one of the three genes in Group I did not significantly affect acetate-dependent growth, suggesting …

Rules Of Engagement: Interspecies Interactions That Regulate Microbial Communities, Ainslie Little, Courtney Jaime Robinson, S Brook Peterson, Kenneth F. Raffa, Jo Handelsman

Courtney Robinson

Writing Research Proposal: Literature Review And Database Search, Mamoudou H. Dicko Prof.

Pr. Mamoudou H. DICKO, PhD

Presence Of A Polyadenylated Rna Fragment Encoding The Membrane Domain For Immunoglobulin Alpha Chain Indicates That Mrnas For Both Secreted And Membrane-Bound Alpha Chains Can Be Produced From The Same Rna Transcript, Janet Stavnezer

Janet M. Stavnezer

RNA blotting was employed to examine polyadenylated immunoglobulin alpha chain RNAs in a B lymphoma synthesizing membrane-bound and secretory IgA and in a hybridoma which synthesizes predominantly secretory IgA. Both cell lines were derived from the I.29 lymphoma and expressed the identical heavy chain variable region gene. In addition to the predicted mRNA precursors, four novel species of polyadenylated alpha RNAs were detected. The presence of a RNA species which was too large to have the same 3' end as the largest mRNA for membrane-bound alpha chain (alpha m) implied that transcription continued past the alpha m poly(A) site, and …

Isolation And Characterization Of Vibrio Tubiashii Outer Membrane Proteins And Determination Of A Toxr Homolog, Broderick Eribo

Broderick Eribo

Elucidation Of An Alternate Isoleucine Biosynthesis Pathway In Geobacter Sulfurreducens, Derek Lovley, Carla Risso, Stephen J. Van Dien, Amber Orloff, Maddalena V. Coppi

Carla Risso

The central metabolic model for Geobacter sulfurreducens included a single pathway for the biosynthesis of isoleucine that was analogous to that of Escherichia coli, in which the isoleucine precursor 2-oxobutanoate is generated from threonine. 13C labeling studies performed in G. sulfurreducens indicated that this pathway accounted for a minor fraction of isoleucine biosynthesis and that the majority of isoleucine was instead derived from acetyl-coenzyme A and pyruvate, possibly via the citramalate pathway. Genes encoding citramalate synthase (GSU1798), which catalyzes the first dedicated step in the citramalate pathway, and threonine ammonia-lyase (GSU0486), which catalyzes the conversion of threonine to 2-oxobutanoate, were …

Temporal Evolution Of Methane Cycling And Phylogenetic Diversity Of Archaea In Sediments From A Deep-Sea Whale Fall In Monterey Canyon (Ca), Shana K. Goffredi, Regina Wilpiszeski, Ray Lee, Victoria J. Orphan

Shana Goffredi

Whale-falls represent localized areas of extreme organic enrichment in an otherwise oligotrophic deep-sea environment. Anaerobic remineralization within these habitats is typically portrayed as sulfidogenic; however, we demonstrate that these systems are also favorable for diverse methane-producing archaeal assemblages, representing up to 40% of total cell counts. Chemical analyses revealed elevated methane and depleted sulfate concentrations in sediments under the whale-fall, as compared to surrounding sediments. Carbon was enriched (up to 3.5%) in whale-fall sediments, as well as the surrounding sea floor to at least 10 m, forming a ‘bulls eye’ of elevated carbon. The diversity of sedimentary archaea associated with …

Identifying The Fundamental Units Of Bacterial Diversity: A Paradigm Shift To Incorporate Ecology Into Bacterial Systematics, A. F. Koeppel, E. B. Perry, J. Sikorski, A. Warner, D. M. Ward, A. P. Rooney, E. Brambilla, N. Connor, E. Nevo, R. M. Ratcliff, Frederick M. Cohan

Frederick M. Cohan

No abstract provided.

Development Of Methods For The Genetic Manipulation Of Flavobacterium Columnare, David R. Nelson

David R. Nelson

Background: Flavobacterium columnare is the causative agent of columnaris disease, a disease affecting many freshwater fish species. Methods for the genetic manipulation for some of the species within the Bacteroidetes, including members of the genus Flavobacterium, have been described, but these methods were not adapted to work with F. columnare. Results: As a first step toward developing a robust set of genetic tools for F. columnare, a protocol was developed to introduce the E. coli – Flavobacterium shuttle vector pCP29 into F. columnare strain C#2 by conjugal mating at an efficiency of 1.5 × 10-3 antibiotic-resistant transconjugants per recipient cell. …

Effect Of Two Milling Techniques And Flours Particle Size On Some, Theodore N'Dede Djeni

Theodore N'Dede Djeni

Two techniques of milling (milling with wooden mortar and milling with hammer grinder) for the production of millet flours were compared in term of physical, biochemical and nutritional aspects and digestibility of the produced flours. A batch of millet grains with candle (Pennisetum glaucum L. R.BR) was purchased from a local market for flour production, while the flour digestibility tests were achieved with an enzymatic crude extract get from gastric juice of and snails (Archachatina marginata). The different rough flours obtained from both milling techniques showed significant differences (p<0.05) in their granulometric profiles and their biochemical composition. The mortar …

Impact Of An External Energy On Yersinia Enterocolitica [Atcc –23715] In Relation To Antibiotic Susceptibility And Biochemical Reactions: An Experimental Study, Mahendra Kumar Trivedi, Shrikant Patil

Mahendra Kumar Trivedi