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Methodology Of In Situ Grazing Experiments: Evaluation Of A New Vital Dye For Preparation Of Fluorescently Labeled Bacteria, Slava S. Epstein, Jeffrey Rossel

Slava Epstein

A new fluorescent dye, cyanoditolyl tetrazolium chloride (CTC), is suggested for use in grazing studies to prepare fluorescently labeled bacteria (FLB). As food tracers, CTC-stained bacteria appeared to have advantages over FLBs prepared in accordance with previously published protocols. The CTC is a vital dye, non-toxic to the examined bacteria; in our study, the CTC-stained bacteria grew at the same rate as non-stained bacteria. This is an advantage over the commonly used DTAF-employing staining protocol, which results in heat-killed or immobilized prey particles. The CTC-stained bacteria were not toxic to the studied bacterial grazers; a test ciliate grew on FLBs …