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The Helicobacter Pylori Flaa1 And Wbpb Genes Control Lipopolysaccharide And Flagellum Synthesis And Function, Alexandra Merkx-Jacques, R. Obhi, G. Bethune, C. Creuzenet
The Helicobacter Pylori Flaa1 And Wbpb Genes Control Lipopolysaccharide And Flagellum Synthesis And Function, Alexandra Merkx-Jacques, R. Obhi, G. Bethune, C. Creuzenet
Alexandra Merkx-Jacques
flaA1 and wbpB are conserved genes with unknown biological function in Helicobacter pylori. Since both genes are predicted to be involved in lipopolysaccharide (LPS) biosynthesis, flagellum assembly, or protein glycosylation, they could play an important role in the pathogenesis of H. pylori. To determine their biological role, both genes were disrupted in strain NCTC 11637. Both mutants exhibited altered LPS, with loss of most O-antigen and core modification, and increased sensitivity to sodium dodecyl sulfate compared to wild-type bacteria. These defects could be complemented in a gene-specific manner. Also, flaA1 could complement these defects in the wbpB mutant, suggesting a …