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Gel Shift Analysis Of The Empa Promoter Region In Vibrio Anguillarum, Steven Denkin, Pedja Sekaric, David Nelson
Gel Shift Analysis Of The Empa Promoter Region In Vibrio Anguillarum, Steven Denkin, Pedja Sekaric, David Nelson
David R. Nelson
BACKGROUND: The induction of metalloprotease encoded by empA in Vibrio anguillarum occurs at high cell density in salmon intestinal mucus. Previously we have shown that there are significant differences in empA expression in two strains of V. anguillarum, M93Sm and NB10. It is hypothesized that differences in empA regulation are due to differences in binding of regulatory elements.
RESULTS: Two strains of V. anguillarum, M93Sm and NB10, were examined and compared for the presence of DNA regulatory proteins that bind to and control the empA promoter region. Gel mobility shift assays, using a digoxigenin (DIG)-labeled oligomer containing a lux box-like …