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Articles 1 - 25 of 25
Full-Text Articles in Life Sciences
The Effect Of Alcalase Concentration On The Proteins From The Shells Of Litopenaeus Setiferus (White Shrimp), Liam T. Quan
The Effect Of Alcalase Concentration On The Proteins From The Shells Of Litopenaeus Setiferus (White Shrimp), Liam T. Quan
Journal of the South Carolina Academy of Science
Chitin is a naturally abundant polymer that also happens to be biodegradable. Chitin can be used in a variety of different products such as biodegradable plastics, papers, medical products, foods, and medical treatments. To extract chitin, shells must be demineralized and deproteinized. The goal of this experiment was to examine the effect of the protease Alcalase in the deproteinization of litopenaeus setiferus shells. The hypothesis was that if the concentration of Alcalase increased, then the absorbance of proteins in the spectrophotometer reading would increase. The null hypothesis was that if the concentration increased there would be no change in absorption. …
Effects Of Using Processed Barley And Supplemented Multi-Enzymes In Laying Hen Rations On Egg Production, Egg Quality And Egg Fatty Acids, Yavuz Gürbüz, Osman Özyürür
Effects Of Using Processed Barley And Supplemented Multi-Enzymes In Laying Hen Rations On Egg Production, Egg Quality And Egg Fatty Acids, Yavuz Gürbüz, Osman Özyürür
Turkish Journal of Veterinary & Animal Sciences
In this study, we have observed different technological processes, which are commonly used in poultry production. Rations contained different amounts of barley and multi-enzyme, egg weight, egg mass, egg yield, feed intake, egg weight. The rations of the control groups were as follows: 0% barley (based of corn) (K), 15% untreated barley (A1), 15% pellet barley (A2), 15% flaked barley (A3), 30% untreated barley (A4), 30% pelleted barley (A5), 30% flaked barley (A6), 30% untreated barley + enzyme (0. 025%) (A7). In the research, 64 brown laying hens ATAK-S for 36 weeks were divided into 8 different treatments for egg …
The N-Terminal Methyltransferase Homologs Nrmt1 And Nrmt2 Exhibit Novel Regulation Of Activity Through Heterotrimer Formation., Jon David Faughn
The N-Terminal Methyltransferase Homologs Nrmt1 And Nrmt2 Exhibit Novel Regulation Of Activity Through Heterotrimer Formation., Jon David Faughn
Electronic Theses and Dissertations
Protein, DNA, and RNA methyltransferases have an ever-expanding list of novel substrates and catalytic activities. Even within families and between homologs, it is becoming clear the intricacies of methyltransferase specificity and regulation are far more diverse than originally thought. In addition to specific substrates and distinct methylation levels, methyltransferase activity can be altered through formation of complexes with close homologs. This work involves the N-terminal methyltransferase homologs NRMT1 and NRMT2. NRMT1 is a ubiquitously expressed distributive trimethylase. NRMT2 is a monomethylase expressed at low levels and in a tissue-specific manner. They are both nuclear methyltransferases with overlapping target consensus sequences …
The Influences Of Feeding Broilers On Graded Inclusion Of Sunflower Meal With Or Without Avizyme On Growth, Protein And Energy Efficiency, Carcass Traits, And Nutrient Digestibility, Mahmoud Alagwany, Adel Attia, Zenat Ibrahim, Mohamed Abd El-Hack1, Muhammed Arif, Mohamed Emam
The Influences Of Feeding Broilers On Graded Inclusion Of Sunflower Meal With Or Without Avizyme On Growth, Protein And Energy Efficiency, Carcass Traits, And Nutrient Digestibility, Mahmoud Alagwany, Adel Attia, Zenat Ibrahim, Mohamed Abd El-Hack1, Muhammed Arif, Mohamed Emam
Turkish Journal of Veterinary & Animal Sciences
The present study aimed to investigate the substituting effect of sunflower meal (SFM) for soybean meal (SBM) with or without supplemental Avizyme on growth performance, protein and energy efficiency ratios, carcass traits, and nutrient digestibility of broiler chickens. A 4 × 2 factorial design experiment was conducted with four levels of SFM replacing SBM (0%, 25%, 50%, 75%) and two levels of supplemented Avizyme (0 or 0.1 g/kg diet). Results revealed that increasing SFM substitution for SBM up to 50% was associated with an increase in body weight (P < 0.01). Daily weight gain was statistically (P < 0.01) enhanced with increasing SFM up to 50% through 22 to 42 and 7 to 42 days of age. Feed conversion ratio improved (P < 0.01) in groups given SFM at 25% and 50% compared to the control. No carcass values were affected by SFM inclusion or Avizyme. The inclusion of 25% or 50% SFM increased (P < 0.01) protein efficiency at periods of 7?21 and 22?42 days of age. Energy efficiency ratio improved (P < 0.01) due to SFM incorporation at levels of 25% or 50% in all periods. The interaction effect between SFM and Avizyme was not significant on a majority of studied parameters. The digestion coefficients of crude fiber, N-free extract, and organic matter increased (P < 0.05 or P < 0.01) with increased levels of SFM. In conclusion, the incorporation of SFM as a substitute for SBM in broiler diets improved the growth performance of broilers. The use of SFM up to 50% without Avizyme supplementation could be recommended in broiler diets from 7 to 42 days of age.
Gross Responses And Apparent Ileal Digestibility Of Amino Acids And Minerals In Broiler Chicken Fed Vegetable-Based Starter Diets Supplemented With Microbial Enzymes, Mohammad Abul Hossain, Paul Ade Iji, Al-Farooq Mohammad Fakhrul Islam
Gross Responses And Apparent Ileal Digestibility Of Amino Acids And Minerals In Broiler Chicken Fed Vegetable-Based Starter Diets Supplemented With Microbial Enzymes, Mohammad Abul Hossain, Paul Ade Iji, Al-Farooq Mohammad Fakhrul Islam
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to investigate the effects of diet and exogenous enzymes on growth responses and micronutrient digestibility of broilers. Day-old broilers (n = 256) were distributed into a 2 x 2 factorial design and were fed on two basal diets: soybean (T1) or canola (T2) meals as such, or supplemented with enzymes up to 21 days. Feed intake (FI) and live weight (LW) to 21 days on the T2 diet was higher (P < 0.001) than that on T1. Feed conversion ratio (FCR) was better (P < 0.01) on T1 than on T2 at 21 days. Enzymes generally improved (P < 0.001) FI, FCR, and LW. The digestibility of histidine was higher (P < 0.05) on T2, but lysine digestibility was higher (P < 0.01) on T1 at 21 days. Histidine, threonine, lysine, valine, isoleucine, and phenylalanine digestibility was improved (P < 0.05) in the supplemented diets. Mineral digestibility was unaffected (P > 0.05) by diet, but enzymes increased (P < 0.05) digestibility of P, K, Mn, and Cu at 21 days. The digestibility of Cu, Zn, and Mg was higher (P < 0.05) on T2, whereas Ca digestibility was greater (P < 0.05) on the T1 diet. Broilers' growth responded positively to enzyme diets, probably due to improvement in nutrient digestibility.
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Turkish Journal of Veterinary & Animal Sciences
Phospholipase D (PLD) is a major virulence determinant of Corynebacterium pseudotuberculosis. Various studies have focused on the expression of recombinant PLD (rPLD) enzyme in different Escherichia coli strains, but generally a high yield of insoluble protein was reported. The aims of this study were to express soluble rPLD by different methods in E. coli. The rPLD and natural PLD (dPLD) enzymes were concentrated using an ultramembrane cassette system after the efficiencies of these concentrated enzymes were compared. The rPLD enzyme was expressed in One Shot®BL21(DE3) E. coli when induced by IPTG in TY medium. Soluble dPLD and rPLD enzyme hemolytic …
Enzyme Catalyzed Alginate Nanogels For Drug Delivery, Danna Nichole Sharp
Enzyme Catalyzed Alginate Nanogels For Drug Delivery, Danna Nichole Sharp
Masters Theses
Developing nanoscale carriers for the delivery of therapeutics is an important topic of investigation in current biomedical research. As opposed to traditional drug delivery systems, nanoscale systems offer enhanced tissue and cell permeation in addition to reducing drug elimination from the body. Biological based therapeutics such as DNA and proteins are now widely employed in medical applications and research has focused on using nanoscale drug delivery systems to administer these more effectively. Current synthesis methods of nanoscale biotherapeutic carriers face significant challenges. Among these are creating carriers with: sizes between 10-200 nm, low polydispersity, and non-cytotoxic materials. In this thesis, …
Rescuing Acetylcholinesterase From Nerve Agent Inhibition: Protein Dynamics Driven Drug Discovery, Aiyana M. Emigh, Brian Bennion
Rescuing Acetylcholinesterase From Nerve Agent Inhibition: Protein Dynamics Driven Drug Discovery, Aiyana M. Emigh, Brian Bennion
STAR Program Research Presentations
Severe morbidity and mortality consequences result from irreversible inhibition of human acetylcholinesterase by organophosphates (OPs). Oxime-based reactivators are currently the only available treatments but lack efficacy in the central nervous system (CNS) where the most damage occurs. Computational docking and molecular dynamics (MD) simulations reveal complex structural barriers that may reduce oxime efficacy. These results may guide future drug designs of more effective countermeasures.
Lapg, Required For Modulating Biofilm Formation By Pseudomonas Fluorescens Pf0-1, Is A Calcium-Dependent Protease, Chelsea D. Boyd, Debashree Chatterjee, Holger Sondermann, George A. O'Toole
Lapg, Required For Modulating Biofilm Formation By Pseudomonas Fluorescens Pf0-1, Is A Calcium-Dependent Protease, Chelsea D. Boyd, Debashree Chatterjee, Holger Sondermann, George A. O'Toole
Dartmouth Scholarship
Biofilm formation by Pseudomonas fluorescens Pf0-1 requires the cell surface adhesin LapA. We previously reported that LapG, a periplasmic cysteine protease of P. fluorescens, cleaves the N terminus of LapA, thus releasing this adhesin from the cell surface and resulting in loss of the ability to make a biofilm. The activity of LapG is regulated by the inner membrane-localized cyclic-di-GMP receptor LapD via direct protein-protein interactions. Here we present chelation and metal add-back studies demonstrating that calcium availability regulates biofilm formation by P. fluorescens Pf0-1. The determination that LapG is a calcium-dependent protease, based on in vivo and in vitro …
Structural Characterization Of A Conserved, Calcium-Dependent Periplasmic Protease From Legionella Pneumophila, Debashree Chatterjee, Chelsea D. Boyd, George A. O'Toole, Holger Sondermann
Structural Characterization Of A Conserved, Calcium-Dependent Periplasmic Protease From Legionella Pneumophila, Debashree Chatterjee, Chelsea D. Boyd, George A. O'Toole, Holger Sondermann
Dartmouth Scholarship
The bacterial dinucleotide second messenger c-di-GMP has emerged as a central molecule in regulating bacterial behavior, including motility and biofilm formation. Proteins for the synthesis and degradation of c-di-GMP and effectors for its signal transmission are widely used in the bacterial domain. Previous work established the GGDEF-EAL domain-containing receptor LapD as a central switch in Pseudomonas fluorescens cell adhesion. LapD senses c-di-GMP inside the cytosol and relays this signal to the outside by the differential recruitment of the periplasmic protease LapG. Here we identify the core components of an orthologous system in Legionella pneumophila. Despite only moderate sequence conservation at …
Crystal Structure Of The Cystic Fibrosis Transmembrane Conductance Regulator Inhibitory Factor Cif Reveals Novel Active-Site Features Of An Epoxide Hydrolase Virulence Factor, Christopher D. Bahl, Christophe Morisseau, Jennifer M. Bomberger, Bruce A. Stanton, Bruce D. Hammock, George A. O'Toole, Dean R. Madden
Crystal Structure Of The Cystic Fibrosis Transmembrane Conductance Regulator Inhibitory Factor Cif Reveals Novel Active-Site Features Of An Epoxide Hydrolase Virulence Factor, Christopher D. Bahl, Christophe Morisseau, Jennifer M. Bomberger, Bruce A. Stanton, Bruce D. Hammock, George A. O'Toole, Dean R. Madden
Dartmouth Scholarship
Cystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. Initial sequence analysis suggested that Cif is an epoxide hydrolase (EH), but its sequence violates two strictly conserved EH motifs and also is compatible with other alpha/beta hydrolase family members with diverse substrate specificities. To investigate the mechanistic basis of Cif activity, we have determined its structure at 1.8-A resolution by X-ray crystallography. The catalytic triad consists of residues Asp129, His297, and Glu153, which are conserved across the …
Transposon Disruption Of The Complex I Nadh Oxidoreductase Gene (Snod) In Staphylococcus Aureus Is Associated With Reduced Susceptibility To The Microbicidal Activity Of Thrombin-Induced Platelet Microbicidal Protein 1, Arnold S. Bayer, Peter Mcnamara, Michael R. Yeaman, Natalie Lucindo, Tiffanny Jones, Ambrose L. Cheung
Transposon Disruption Of The Complex I Nadh Oxidoreductase Gene (Snod) In Staphylococcus Aureus Is Associated With Reduced Susceptibility To The Microbicidal Activity Of Thrombin-Induced Platelet Microbicidal Protein 1, Arnold S. Bayer, Peter Mcnamara, Michael R. Yeaman, Natalie Lucindo, Tiffanny Jones, Ambrose L. Cheung
Dartmouth Scholarship
The cationic molecule thrombin-induced platelet microbicidal protein 1 (tPMP-1) exerts potent activity against Staphylococcus aureus. We previously reported that a Tn551 S. aureus transposon mutant, ISP479R, and two bacteriophage back-transductants, TxA and TxB, exhibit reduced in vitro susceptibility to tPMP-1 (tPMP-1(r)) compared to the parental strain, ISP479C (V. Dhawan, M. R. Yeaman, A. L. Cheung, E. Kim, P. M. Sullam, and A. S. Bayer, Infect. Immun. 65:3293-3299, 1997). In the current study, the genetic basis for tPMP-1(r) in these mutants was identified. GenBank homology searches using sequence corresponding to chromosomal DNA flanking Tn551 mutant strains showed that the fourth gene …
Performance And Some Digesta Parameters Of Broiler Chickens Given Low Or High Viscosity Wheat-Based Diets With Or Without Enzyme Supplementation, Mevlüt Günal, Sulhatti̇n Yaşar, John Mike Forbes
Performance And Some Digesta Parameters Of Broiler Chickens Given Low Or High Viscosity Wheat-Based Diets With Or Without Enzyme Supplementation, Mevlüt Günal, Sulhatti̇n Yaşar, John Mike Forbes
Turkish Journal of Veterinary & Animal Sciences
The influence of 2 enzyme supplements for diets based on wheat with high or low viscosity on performance and some intestinal parameters of male broiler chickens were investigated. The experiment was of 2 x 3 factorial design, with 2 types of wheat (high or low viscosity) and 3 enzyme treatments (none, Avizyme 1300-xylanase or Avizyme 1500-amylase). Ileal digesta viscosity was significantly (P < 0.029) higher in birds fed with high viscous wheat than with low viscous wheat. Ileal digesta viscosity (P < 0.001) and dry matter content (P < 0.002) were significantly reduced by both enzyme preparations. Body weight gain, feed intake, dry matter intake, feed conversion ratio, DM retention and wet or dry excreta output were not significantly (P > 0.05) affected by all treatments. There were no significant interactions (P > 0.05) between the effects of wheat type and enzyme supplementation. In conclusion, the supplementation of high or low viscous wheat-based diets with the enzyme preparations of amylase …
The Effects Of Triticale Used In Different Amounts In Laying Quail Rations On Egg Production And Egg Quality, Berri̇n Kocaoğlu Güçlü, Kaan M. İşçan
The Effects Of Triticale Used In Different Amounts In Laying Quail Rations On Egg Production And Egg Quality, Berri̇n Kocaoğlu Güçlü, Kaan M. İşçan
Turkish Journal of Veterinary & Animal Sciences
This study was performed to determine the effects of using 40% and 60% triticale with or without enzyme instead of maize on egg production, feed intake, feed efficiency, egg quality and hatchability in laying quail diets. In this study, a total of 160, 12-week-old Japanese quails (Coturnix coturnix japonica) were used; birds with similar live weight and production levels were divided into five groups of 32 quails. Although using triticale at the rate of 40% and 60% in the diet of laying quails did not affect egg production, hatchability, fertility, egg specific gravity, egg shell thickness, the egg white and …
Transformation And Stability Of Cloned Polysaccharidase Genes In Gram-Positive Bacteria, Mehmet Sai̇t Eki̇nci̇, Harry J. Flint
Transformation And Stability Of Cloned Polysaccharidase Genes In Gram-Positive Bacteria, Mehmet Sai̇t Eki̇nci̇, Harry J. Flint
Turkish Journal of Veterinary & Animal Sciences
Polysaccharidase genes from rumen bacteria were transferred to and expressed in ruminal and non-ruminal Gram-positive bacteria. The transformation efficiency and genetic stability of polysaccharidase genes in bacteria from different habitats were investigated. PCR amplification of cloned polysaccharidase genes from Escherichia coli, Lactococcus lactis, Enterococcus faecalis, Streptococcus sanguis and S. bovis strain 26 showed that rearrangement of plasmid and the gene fragment did not occur. The DNA band sizes from all primers agreed with the expectations of some transformants of S. bovis JB1 being rearranged. In spite of the rearrangement in S. bovis JB1 polysaccharidase activities were conserved. Growing in the …
The Effects Of Lactic Acid Bacteria And Lactic Acid Bacteria+Enzyme Mixture Silage Inoculants On Maize Silage, İsmai̇l Fi̇lya
The Effects Of Lactic Acid Bacteria And Lactic Acid Bacteria+Enzyme Mixture Silage Inoculants On Maize Silage, İsmai̇l Fi̇lya
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to determine the effects of lactic acid bacteria and lactic acid bacteria+enzyme mixture inoculants as silage additives, on the fermentation, aerobic stability, cell wall content, and in situ rumen degradability of maize (Zea mays) silage. H/M F Inoculant No. 9927 (Medipharm, USA), and Sil-All (Alltech, UK) were used as lactic acid bacteria and lactic acid bacteria+enzyme mixture inoculants. Inoculants were applied to 106cfu g-1 silage levels. Maize was harvested at dent stages of maturity and ensiled in 1.5 liter special jars equipped with a lid that enabled gas release only. The jars were stored at …
Expression Of Bifunctional Genes Encoding Xylanase And \Beta(1,3-1,4)-Glucanase In Gram-Positive Bacteria, Mehmet Sai̇t Eki̇nci̇, Harry J. Flint
Expression Of Bifunctional Genes Encoding Xylanase And \Beta(1,3-1,4)-Glucanase In Gram-Positive Bacteria, Mehmet Sai̇t Eki̇nci̇, Harry J. Flint
Turkish Journal of Veterinary & Animal Sciences
The xylanase and \beta(1,3-1,4)-Glucanase activities encoding gene from the cellulolytic rumen anaerobe bacterium was cloned into Escherichia/Streptococcus shuttle vector for introduction into Gram-positive bacteria, including the rumen facultative anaerobe bacterium Streptococcus bovis. Activities due to the cloned gene decreased in the stationary phase in batch cultures of S. bovis, reflecting the sensitivity of the cloned enzymes to inactivation in the presence of accumulated lactic acid. Cloned gene activity was detected in the culture supernatant, indicating recognition of the cloned gene signal peptide by Gram-positive bacteria.
The Effects Of Enzyme And Probiotic Supplementation To Diets On Broiler Performance, Mustafa Mi̇di̇lli̇, Şaki̇r Doğan Tuncer
The Effects Of Enzyme And Probiotic Supplementation To Diets On Broiler Performance, Mustafa Mi̇di̇lli̇, Şaki̇r Doğan Tuncer
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to determine the effect of the supplementation of enzyme and probiotic to broiler feeds based on barley and wheat on broiler performance, dressing percentage, the small intestinal length and weight, liver and abdominal fat weights, intestinal viscosity and the percentage of sticky droppings. In this experiment, 600 one - day - old Hubbard broiler chicks were used. This study was performed on four treatment groups, each containing 150 chicks. One of the groups was the control group and the others were the experimental groups. Each treatment group was divided into 6 replicates of 25 chicks. …
Effects Of Enzyme Supplementation To Corn Or Wheat Based Diets Containing Low Energy And Protein On Broiler Chick Performance, Ergün Demi̇r
Effects Of Enzyme Supplementation To Corn Or Wheat Based Diets Containing Low Energy And Protein On Broiler Chick Performance, Ergün Demi̇r
Turkish Journal of Veterinary & Animal Sciences
Two experiments were carried out in order to determine the effect of corn-soybean meal or wheat-soybean meal based diets containing low energy (DE) and low energy-low protein (DEDP) supplemented with enzyme on the performance of broiler chicks between 0 and 21 days of age. In the first experiment, three diets, namely, control, low energy (DE) and low energy-protein (DEDP) based on corn-soybean meal, were formulated. In the second experiment, control and three diets containing 40% wheat which have normal energy-protein (NENP), low energy (DE) and low energy-protein (DEDP) levels were used. The enzyme was added to the experimental diets, except …
Use Of Sunflower Meal With Enzyme Mixture Substituted For Soybean Meal In Broiler Diets, Fi̇gen Kirkpinar, Hati̇ce Basmacioğlu
Use Of Sunflower Meal With Enzyme Mixture Substituted For Soybean Meal In Broiler Diets, Fi̇gen Kirkpinar, Hati̇ce Basmacioğlu
Turkish Journal of Veterinary & Animal Sciences
The study was carried out to investigate the effects of sunflower meal with an enzyme mixture containing \alpha-amylase, ß-glucanase, cellulase, lipase and protease substituted for soybean meal in broiler diets on performance, intestinal weight, viscosity and pH, as well as the frequency of sticky droppings and abdominal fat. In the trial, a total of 960 one-day-old Avian broiler chicks were used. The trial lasted 6 weeks. A total of 12 experimental diets were supplied ad libitum. Dietary treatments consisted of 15%, 25%, 35% soybean meal, or sunflower meal or sunflower meal with the enzyme mixture (0.05% and 0.10%). Enzyme supplementation …
The Effects Of Danofloxacin On Some Biochemical Parameters And Liver In Broilers, Fatma Uyanik, Bi̇lal Cem Li̇man, Nari̇n Li̇man
The Effects Of Danofloxacin On Some Biochemical Parameters And Liver In Broilers, Fatma Uyanik, Bi̇lal Cem Li̇man, Nari̇n Li̇man
Turkish Journal of Veterinary & Animal Sciences
In this study, a total of 160 one day old broiler chicks were devided into two groups consisting of 60 chicks in control group and 100 chicks in treatment group. The birds in treatment group received 50 ppm, 25 ppm, 37.5 ppm and 50 ppm danofloxacin via drinking water between the days during 0-7, 8-21, 22-35 and 36-42 respectively. Live weight, liver weight, serum total protein, albumin, globulin, copper, zinc and magnesium levels as well as \gamma-GT, GOT and GPT activities of 15 birds from control group and 25 birds from treatment group were determined on days 15, 21, 35 …
Effect Of Different Enzyme Supplementation On The Performance And Ileal Ph Of Broilers Fed Wheat-And Barley-Based Diets, Müjdat Alp, Recep Kahraman, Neşe Kocabağli, İsmai̇l Abaş, Harun Aksu
Effect Of Different Enzyme Supplementation On The Performance And Ileal Ph Of Broilers Fed Wheat-And Barley-Based Diets, Müjdat Alp, Recep Kahraman, Neşe Kocabağli, İsmai̇l Abaş, Harun Aksu
Turkish Journal of Veterinary & Animal Sciences
This experiment was conducted to study the effects of different enzyme supplementation to wheat-and barley-based diets on the live weight gain, feed efficiency, dressing percentage and ileal pH of broiler chickens. Two hundred fourtyfive, day-old Ross-PM3 chicks were randomly assigned to a control and four treatment groups. Starter (1 to 21 days old) and grower (22 to 42 days old) diets were given without (control) or with different enzyme preparations, which are Avizyme 1200 (1000 mg/kg), Kemzyme Dry (500 mg/kg), Roxazyme-G (200 mg/kg) and Wheatzyme (700 mg/kg). At the end of the six week, average live weights of broilers were …
Interleukin-1 Or Phorbol Induction Of The Stromelysin Promoter Requires An Element That Cooperates With Ap-1., Karen Sirum-Connolly, Constance E. Brinckerhoff
Interleukin-1 Or Phorbol Induction Of The Stromelysin Promoter Requires An Element That Cooperates With Ap-1., Karen Sirum-Connolly, Constance E. Brinckerhoff
Dartmouth Scholarship
Interieukin-1, a mediator of inflammation, or tumor promoting phorbol esters induce transcription of stromelysin, a metalloproteinase that degrades extracellular matrix molecules and that is overexpressed in diseases such as rheumatoid arthritis. Sequences required for induction of transcription of the human stromelysin promoter are contained on a 46 base pair fragment. This fragment contains a sequence with a high degree of similarity to the binding site for the transcription factor activator protein-1 (AP-1) and indeed, the AP-1 sequence of this fragment is necessary but not sufficient for the maximal response to phorbol 12-myristate 13-acetate (phorbol) or interleukin-1. Maximal induction requires functional …
Simian Virus 40 Host Range/Helper Function Mutations Cause Multiple Defects In Viral Late Gene Expression., Terryl Stacy, Michele Chamberlain, Charles N. Cole
Simian Virus 40 Host Range/Helper Function Mutations Cause Multiple Defects In Viral Late Gene Expression., Terryl Stacy, Michele Chamberlain, Charles N. Cole
Dartmouth Scholarship
Simian virus 40 (SV40) deletion mutants dlA2459 and dlA2475 express T antigens that lack the normal carboxy terminus. These mutants are called host range/helper function (hr/hf) mutants because they form plaques at 37 degrees C on BSC-1 and Vero monkey kidney cell lines but not on CV-1p monkey kidney cells. Wild-type SV40 can provide a helper function to permit growth of human adenoviruses in monkey kidney cells; the hr/hf mutants cannot. Progeny yields of hr/hf mutants are also cold sensitive in all cell lines tested. Patterns of viral macromolecular synthesis in three cell lines (Vero, BSC-1, and CV-1) at three …
Regulation Of Queuine Insertion Into Transfer Rna: Effects Of Tumor Promoters, Bonnie Jean Brooks
Regulation Of Queuine Insertion Into Transfer Rna: Effects Of Tumor Promoters, Bonnie Jean Brooks
Chemistry & Biochemistry Theses & Dissertations
The purpose of this study was to treat normal human fibroblasts with known tumor promoters and observe queuine modification of tRNA. The queuine insertion enzyme, tRNAguanine ribosyltransferase, was studied in vivo and in vitro. Tumor promoter-treated human fibroblast cultures exhibited variable queuine-insertion rates with a transient inhibition that correlated with variable levels of queuine modified tRNA over time in culture from passages 3 — 8. In contrast the in vitro studies showed that phorbol cetera and saccharin actually increased insertion with strong evidence indicating phosphorylation as a positive modulating force of the enzyme activity. It is proposed that chronic stimulation …