Life Sciences Commons^™

Determination Of Some Of The Lectins' Immunohistochemistry Profile Of The Dog Testisand Epididymis, Feyza Başak, Yeşi̇m Akaydin Bozkurt, Roman Dabrowski

Turkish Journal of Veterinary & Animal Sciences

Lectins are substances composed of proteins or glycoproteins that specifically bond to carbohydrates on the cell surfaces or inside the cell. Lectins are used to identify the carbohydrate types in the cellular membrane or cytoplasm. The aim of the study was to identify the location and distribution of the four types of lectins PNA (Peanut agglutinin- Arachis hypogea), WGA (Wheatgerm Agglutinin- Triticum vulgaris), Con A (Conconavalin A-Canavalia ensiformis), and SBA (Soybean Agglutinin-Glycine max) in dog testes and epididymites. The testes and the body of 10 adult dogs were collected from an animal shelter after castration. Following the standard histological procedures, …

Determination Of Acrylamide Using The Immuno-Enzymatic Method In Commercial Dog And Cat Foods, Ni̇met Kiliç, Bi̇lal Cem Li̇man

Turkish Journal of Veterinary & Animal Sciences

Pet animals raised by humans do not have the chance to choose their own food. Therefore, mistakes made by humans in feeding dogs and cats will adversely affect the health of the animal. There are applications at high temperatures at various stages during the production of dog and cat foods. It is predicted that acrylamide (AA) can be formed as a result of a possible Maillard reaction since the cat and dog foods contain starch in its structure. The aim of this study is to determine the AA residue immuno-enzymatically in dry cat and dog feeds from different companies available …

Clinical Results Of Triple Tibial Osteotomy Treatment In Cranial Cruciateligament-Deficient Dogs, Mustafa Volkan Yaprakci, Zülfükar Kadi̇r Saritaş

Turkish Journal of Veterinary & Animal Sciences

Canine cranial cruciate ligament ruptures have become common with improved nutrition and nonselective breeding strategies. This study assessed 7 dogs of various breeds and ages with the diagnosis of cranial cruciate ligament ruptures. The triple tibial osteotomy (TTO) method was used to treat the rupture. All of the dogs were admitted to the clinic for varying degrees of lameness. Diagnosis was confirmed using both clinical and radiologic examinations. Body weights of the dogs were between 12 and 65 kg. Informed consent was given by the owners prior to surgery. Postoperatively, early evaluations were made using periodic radiographs and clinical examinations, …

Application Of Billroth Ii Gastrojejunostomy And A Biliary Stent In A Dog With Gastric Adenocarcinoma And The Associated Extrahepatic Biliary Obstruction, Da Eun Lee, Ha Eun Lee, Soon Wuk Jeong, Hun Young Yoon

Turkish Journal of Veterinary & Animal Sciences

An 11-year-old intact female chow chow dog was presented for evaluation of intermittent vomiting and anorexia for a month. On ultrasonographic examination, gastric pylorus hypertrophy which compressed common bile duct was identified. The gallbladder was significantly enlarged and mucocele was suspected. Cholecystectomy and gastric full thickness biopsy were performed and gastric adenocarcinoma was diagnosed by histopathological examination. Billroth II gastrojejunostomy using thoracoabdominal-stapler (TAstapler) was performed. On surgery, hypertrophied tissue suspected of tumour was also found adjacent to the major duodenal papilla. After surgery, vomiting disappeared, and the patient regained appetite. On postoperative day 90, the patient was presented vomiting and …

Cerebral Metastasis Of Transmissible Venereal Tumor After Effective Chemotherapy In A Dog, Gabriela Silva Hidalgo, Silvia Del Carmen Cota Guajardo, Margarita Del Socorro Davila Paredes, Nora Maria Carcamo Arechiga, Martin Lopez Valenzuela

Turkish Journal of Veterinary & Animal Sciences

A male mixed breed street dog (age unknown) presented with an ocular mass and multiple ulcerated subcutaneous nodules on both hind limbs which bled easily when touched. Inspection of the genital organs revealed that no masses were present. The ocular mass and nodules were diagnosed as a transmissible venereal tumor (TVT) by fine-needle aspiration cytology. The total remission of the tumor was achieved after 5 applications of vincristine sulfate. The dog was adopted during the remission period, but 5 months after adoption he developed neurological signs and died. The necropsy revealed a mass located in the trunk of the corpus …

Stress Response Related To Ultrasonographic Examination In Dogs, Cenker Çağri Cingi, Abdurrahman Fati̇h Fi̇dan, Durmuş Fati̇h Başer, Mehmet Uçar, Zülfükar Kadi̇r Saritaş

Turkish Journal of Veterinary & Animal Sciences

This study investigated whether abdominal ultrasonography, a frequently used examination method in clinics and hospitals, causes stress in dogs. For the study, 10 healthy dogs were used. Following general examination, intravenous catheters were placed in the V. cephalica accecorius of the dogs and blood specimens were collected to obtain serum and plasma samples (minute 0). After a 30- min break, ultrasonography was applied following shaving of the abdominal region. Serum and plasma samples were collected from the intravenous catheters at the 5th, 15th, and 20th minutes of the ultrasonographic examination during which the urinary bladder, left kidney, spleen, stomach, liver, …

Evaluation Of Morgan's Pocket Technique In The Treatment Of Nictitans Gland Prolapse In Dogs, Mehmet Zeki̇ Yilmaz Deveci̇, Cafer Tayer İşler, Zi̇ya Yurtal, Muhammed Enes Altuğ, Ömer Kirgiz

Turkish Journal of Veterinary & Animal Sciences

Nictitans gland prolapse (NGP), also known as third eyelid gland prolapse or cherry eye disease, is an important eye problem that should be treated as soon as possible in dogs. Although many surgical methods have been described, there is no consensus on the most effective technique. In this study, it was aimed to evaluate the characteristics of cases and clinical outcomes of Morgan's pocket technique in the treatment of dogs with NGP. In this context, the characteristics of the cases were examined in 25 dogs diagnosed (29 eyes) with NGP. Clinical results, recurrence, and complications were evaluated in 17 dogs …

In Vitro Investigation Of Netosis Reaction Developing From Dog Polymorphonuclearneutrophils To Toxoplasma Gondii, Güneş Karakurt, Kader Yildiz

Turkish Journal of Veterinary & Animal Sciences

Toxoplasma gondii is known to develop extracellular traps from neutrophils in some animal species such as mice, cattle, sheep, cats, and donkeys. This study aimed to investigate the extracellular trap structures formed in dog polymorphonuclear leukocytes (PMNs) to T. gondii tachyzoites in vitro. Dog PMN was isolated using Percoll dilutions (45%, 54%, 63%, and 72%). After incubation with tachyzoites, the extracellular traps originating from the dog PMNs were observed in the extracellular areas. Histones (H3), myeloperoxidase (MPO), and neutrophil elastase (NE), the characteristic features of NETosis reaction, were detected in extracellular areas. The tachyzoites were observed between the extracellular trap …

The Clinical Outcome Of Three Procedures For Extraarticular Stabilization Of Cranial Cruciate Ligament Injuries In Dogs, Cornel Igna, Roxana Dascalu, Daniel Bumb, Bogdan Sicoe, Cristian Zaha, Larisa Schuszler

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate the long-term outcomes in dogs with cranial cruciate ligament injury in which the stifle was stabilized with lateral suture stabilization, lateral suture stabilization-modified, or Securos lateral bone anchor tibial suture and monofilament nylon sutures placed at quasi-isometric points of the stifle. It is a retrospective study comprising a sample population of 49 client-owned dogs. Medical records from 2014?2016 were reviewed to identify dogs that had a cranial cruciate ligament rupture in which the stifle was stabilized using one of the aforementioned methods. The overall complication rate was 16.3% and the documented complication …

Coproid Predicts The Source Of Coprolites And Paleofeces Using Microbiome Composition And Host Dna Content, Maxime Borry, Bryan Cordova, Angela Perri, Marsha Wibowo, Tanvi Prasad Honap, Jada Ko, Kate Britton, Linus Girdland-Flink, Robert C. Power, Ingelise Stuijts, Domingo C. Salazar-García, Courtney Hofman, Richard Hagan, Thérèse Samdapawindé Kagoné, Nicolas Meda, Helene Carabin, David Jacobson, Karl Reinhard, Cecil Lewis, Aleksandar Kostic, Choongwon Jeong, Alexander Herbig, Alexander Hübner, Christina Warinner

Karl Reinhard Publications

Shotgun metagenomics applied to archaeological feces (paleofeces) can bring new insights into the composition and functions of human and animal gut microbiota from the past. However, paleofeces often undergo physical distortions in archaeological sediments, making their source species difficult to identify on the basis of fecal morphology or microscopic features alone. Here we present a reproducible and scalable pipeline using both host and microbial DNA to infer the host source of fecal material. We apply this pipeline to newly sequenced archaeological specimens and show that we are able to distinguish morphologically similar human and canine paleofeces, as well as non-fecal …

Molecular Investigation And Phylogeny Of Species Of The Anaplasmataceae Infecting Animals And Ticks In Senegal, Mustapha Dahmani, Bernard Davoust, Masse Sambou, Hubert Bassene, Pierre Scandola, Tinhinene Ameur, Didier Raoult, Florence Feenollar, Oleg Mediannikov

Biological Sciences Faculty Publications

Background: Our study aimed to assess the diversity of the species of Anaplasmataceae in Senegal that infect animals and ticks in three areas: near Keur Momar Sarr (northern region), Dielmo and Diop (Sine Saloum, central region of Senegal), and in Casamance (southern region of Senegal).

Methods: A total of 204 ticks and 433 blood samples were collected from ruminants, horses, donkeys and dogs. Ticks were identified morphologically and by molecular characterization targeting the 12S rRNA gene. Molecular characterization of species of Anaplasmataceae infecting Senegalese ticks and animals was conducted using the 23S rRNA, 16S rRNA, rpoB and groEL genes.

Results: …

Canine Dystocia In 50 Uk First-Opinion Emergency Care Veterinary Practices: Clinical Management And Outcomes, Dan G. O'Neill, Aoife M. O'Sullivan, Erin A. Manson, David B. Church, Paul Mcgreevy, Amanda K. Boag, Dave C. Brodbelt

Natology Collection

Canine dystocia is a relatively common veterinary presentation. First opinion emergency care clinical data from 50 Vets Now clinics across the UK were used to explore dystocia management and outcomes in bitches. Caesarean section (CS) was performed on 341/701 (48.6 per cent (95 per cent CI 44.9 to 52.4)) of dystocia cases. The bulldog (OR 7.60, 95 per cent CI 1.51 to 38.26, P=0.014), Border terrier (OR 4.89, 95 per cent CI 0.92 to 25.97, P=0.063) and golden retriever (OR 4.07, 95 per cent CI 0.97 to 17.07, P=0.055) had the highest odds of CS among dystocic bitches compared with …

Treatment With Custom Partial Condyle Prosthesis Of A Comminuted Femoral Condylefracture In A Dog: A Case Report, Kemal Altunatmaz, Ebru Eravci Yalin, Büşra İnal Günay, Defne Joan Şadalak Mckinstry, Zeynep Tol Sevi̇m

Turkish Journal of Veterinary & Animal Sciences

The patient in this case report was a male Golden Retriever dog, approximately 5 years of age, found in woodlands and presenting with an inability to use the right hindlimb. In craniocaudal and mediolateral radiographs taken of the patient, presence of an old comminuted fracture caused by a firearm injury was observed in the right femoral condyle. A custom-made trochlear groove of suitable dimensions for the patient was produced. The patient tip-toed on postoperative day 1 and was seen to be mostly weight-bearing by day 7. The lameness was determined to have lessened to a great extent after 1 month …

A Case Of Ex Vivo Boosted Immune Cell Therapy For Chronic Ulcerative Dermatitis In A Dog, Seulgi Bae

Turkish Journal of Veterinary & Animal Sciences

A 13-year-old castrated male Shih Tzu was presented with recurrent otitis externa and ulcerative dermatitis. Previous investigations had failed to identify the cause, and the lesion was resistant to treatment. According to the histopathological examination, a tentative diagnosis of chronic ulcerative dermatitis with bacterial infection was made. To treat the patient, immune cell therapy with antibiotics was conducted every 2 weeks, for a total of six sessions. After treatment, the skin lesion resolved. No side effects were observed during therapy. To the best of our knowledge, this is the first case report of the use of immune cell therapy for …

Assessment Of Asymmetric Dimethyl Arginine, Cardiac Troponin I, Thyroxine, Cholesterol, And Triglyceride Levels In Obese Dogs And Dogs With Normal Body Condition, Hüseyi̇n Ci̇han, Merve Tural

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to assess serum asymmetric dimethyl arginine (ADMA), cardiac troponin I (cTnI), thyroxine (T4), cholesterol and triglyceride (TG) levels in dogs with normal body condition and obese dogs. A total of 40 dogs (n = 40) were included in the study and dogs were divided into two groups; 20 dogs with normal body condition and 20 obese dogs. The dogs were grouped according to the five-point body-condition scoring (BCS) system. Serum ADMA, cTnI, T4, cholesterol, and TG levels were measured in addition to routine biochemical parameters. Serum ADMA, cTnI, TG, and cholesterol levels were statistically …

Cyanoacrylate Tissue Adhesive For The Management Of Corneal Perforation In Dogs:A Report Of Three Cases, Je-Hwan Jang, Joon Young Kim

Turkish Journal of Veterinary & Animal Sciences

Corneal perforation can cause profound vision loss and requires immediate treatment. Bandage contact lens insertion and gluing are temporary treatment methods. However, if the patient cannot undergo surgery for a variety of reasons, these methods are inevitably used as permanent treatment methods. The use of cyanoacrylate tissue adhesive (CTA) for the management of corneal perforation in humans has been reported for several decades. Here we describe, for the first time as per our knowledge, the use of CTA (Vetbond) for the management of corneal perforation with a diameter of

Abdominal Ultrasonographic Findings In Dogs With Mammary Tumors: Association With Tumor Characteristics And Survival, Daniella Matos Da Silva, Angélica Inajá Juliani, Camila Lopes Ribeiro, Jair Rodini Engracia Filho, Simone Domit Guérios, Tilde Rodrigues Froes

Turkish Journal of Veterinary & Animal Sciences

Abdominal ultrasound is frequently performed as part of initial staging of canine mammary tumors. The aims of this study were to describe abdominal ultrasonographic findings present at diagnosis of mammary tumors in dogs and to assess their value for associations with histopathological results and treatment outcome. The medical records of 201 female dogs diagnosed with mammary tumors that had abdominal ultrasonography performed as part of their initial clinical evaluation were reviewed. Histopathological diagnosis of malignant mammary tumors was obtained in most cases (86%; 172 cases). One hundred and fifty-seven animals (78%) had some ultrasonographic abnormality. A total of 338 ultrasonographic …

Cognitive Dysfunction In Naturally Occurring Canine Idiopathic Epilepsy, Rowena M.A. Packer, Paul D. Mcgreevy, Hannah Salvin, Michael Valenzuela, Chloe M. Chaplin, Holger A. Volk

Neurology Collection

Globally, epilepsy is a common serious brain disorder. In addition to seizure activity, epilepsy is associated with cognitive impairments including static cognitive impairments present at onset, progressive seizure-induced impairments and co-morbid dementia. Epilepsy occurs naturally in domestic dogs but its impact on canine cognition has yet to be studied, despite canine cognitive dysfunction (CCD) recognised as a spontaneous model of dementia. Here we use data from a psychometrically validated tool, the canine cognitive dysfunction rating (CCDR) scale, to compare cognitive dysfunction in dogs diagnosed with idiopathic epilepsy (IE) with controls while accounting for age. An online cross-sectional study resulted in …

Ocular Ultrasonographic Evaluation Of Cataractous And Pseudophakic Eyes In Dogs, Shriram Ganesan, Ramani Chandrasekhara Iyer

Turkish Journal of Veterinary & Animal Sciences

Ocular B-mode ultrasonography is one of the noninvasive, rapid diagnostic imaging techniques indicated in canine cataract patients to evaluate the posterior segment of the eye. This study was conducted on twelve eyes of dogs with a history of cataract, which were scanned under topical anesthesia. The preoperative and postoperative ultrasonographic biometry, and ultrasonographic features of the lens, intraocular lens (IOL), and posterior segment were recorded. There was a significant difference between pre- and postphacoemulsification mean value of the axial length of globe and anterior chamber depth. The normal ultrasonographic features of the 2-hydroxy-ethyl-methylacrylate IOL was observed in vitro by placing …

Bilateral Radial Carpal Bone Luxation And Its Treatment In A Dog, Didar Aydin Kaya

Turkish Journal of Veterinary & Animal Sciences

Radial carpal bone luxation is rarely encountered in dogs. A literature review did not reveal any case of bilateral radial carpal bone luxation or its treatment. In the current case study, the process and result of the treatment of a dog with bilateral radial carpal bone luxation, brought to the İstanbul University Faculty of Veterinary Medicine?s Department of Surgery is presented. Treatment of luxation was achieved by using screws and cerclage wire. The long-term follow-up of this patient was limited to only 2 months since the dog was not brought back to the clinic.

The First Case Of Traumatic Myiasis Caused By Musca Domestica In A Dog In Konya, Turkey, Bi̇lal Di̇k, Ceylan İlhan, Onur Ceylan, Elgi̇n Orçum Uzunlu

Turkish Journal of Veterinary & Animal Sciences

A case of traumatic myiasis caused by the Musca domestica (Linnaeus 1758) housefly was detected in a dog in Konya, Turkey. A 4-year-old Kangal breed dog injured in a traffic accident was brought to the surgery clinic of the Faculty of Veterinary Medicine at Selçuk University in Konya, Turkey for treatment. Several dipterous larvae were collected from the wound of the dog, placed in a petri dish with 70% ethanol, and sent to the parasitology laboratory for identification. The larvae were examined macroscopically. The specimens were identified as third-instar larvae of M. domestica. This is the first report of traumatic …

Severe Granulomatous Hepatitis Caused By Capillaria Hepatica In A Puppy, Ayli̇n Alasonyalilar Demi̇rer, Ahmet Akkoç, Bayram Şenli̇k, İbrahi̇m Taci̇ Cangül

Turkish Journal of Veterinary & Animal Sciences

This report describes the inflammation caused by Capillaria hepatica in the liver of a female stray dog of approximately 2 months old for the first time in Turkey. Histopathological examination revealed severe granulomatous hepatitis with occasional adult nematodes and abundant eggs in the hepatic parenchyma. The present case differs from the other capillariasis cases by the age of the dog and the presence of mature female parasites and severe inflammation.

Thromboelastographic Evaluation Of Hemostatic Functionin Dogs With Dilated Cardiomyopathy, Zeki̇ Yilmaz, Meri̇ç Kocatürk, Oya Eralp İnan, Pinar Levent

Turkish Journal of Veterinary & Animal Sciences

This study aimed to evaluate the hemostatic function by thromboelastography (TEG) and determine if there was an association between the changes in hemostatic parameters and serum cardiac troponin I (cTnI) in dogs with dilated cardiomyopathy (DCM). DCM was diagnosed by echocardiography in 19 dogs. Ten healthy dogs were selected as controls. Myocardial injury was confirmed by increased serum cTnI levels. Coagulation was assessed by TEG evaluating clot kinetics (reaction time [R] and kinetic time [K]), clot strengthening (alpha [α] angle and G value), platelet function (maximum amplitude [MA] and coagulation-index [CI]), clot stability (LY30, the percentage of lysis 30 min …

First Clinical Strongyloides Stercoralis Case In A Dog In Turkey, Şi̇nasi̇ Umur, Yücel Meral, Cenk Soner Bölükbaş, Ali̇ Tümay Gürler, Mustafa Açici

Turkish Journal of Veterinary & Animal Sciences

A 2.5-year-old small male Cavalier King Charles spaniel was brought to Ondokuz Mayıs University's Faculty of Veterinary Medicine Animal Hospital with symptoms of severe watery diarrhea, pain during defecation, intermittent cough, vomiting, and emaciation. Examination of the fecal smear revealed moderate infection of Strongyloides stercoralis larvae and two adult females. Counting of the larvae in feces was performed by Baermann method and 20 larvae were determined per gram of feces. The dog was treated with ivermectin (Avromec, 0.2 mg/kg subcutaneously) and multivitamin syrup (Vitabiol) for 5 days for supporting the body condition. This paper is the first clinical case report …

Aβ Vaccination In Combination With Behavioral Enrichment In Aged Beagles: Effects On Cognition, Aβ, And Microhemorrhages, Paulina R. Davis, Ginevra Giannini, Karin Rudolph, Nathaniel Calloway, Christopher M. Royer, Tina L. Beckett, M. Paul Murphy, Frederick Bresch, Dieter Pagani, Thomas Platt, Xiaohong Wang, Amy Skinner Donovan, Tiffany L. Sudduth, Wenjie Lou, Erin L. Abner, Richard J. Kryscio, Donna M. Wilcock, Edward G. Barrett, Elizabeth Head

Sanders-Brown Center on Aging Faculty Publications

Beta-amyloid (Aβ) immunotherapy is a promising intervention to slow Alzheimer’s disease (AD). Aging dogs naturally accumulate Aβ and show cognitive decline. An active vaccine against fibrillar Aβ 1–42 (VAC) in aged beagles resulted in maintenance but not improvement of cognition along with reduced brain Aβ. Behavioral enrichment (ENR) led to cognitive benefits but no reduction in Aβ. We hypothesized cognitive outcomes could be improved by combining VAC with ENR in aged dogs. Aged dogs (11–12 years) were placed into 4 groups: (1) control/control (C/C); (2) control/VAC (C/V); (3) ENR/control (E/C); (4) ENR and VAC (E/V) and treated for 20 months. …

Fascia Lata Flap To Repair Perineal Hernia In Dogs: A Preliminary Study, Simone Guérios, Juliana Monteiro, Daniella Matos Da Silva

Turkish Journal of Veterinary & Animal Sciences

Perineal hernia (PH) in dogs normally requires surgical reconstruction of the pelvic diaphragm. The use of fascia lata grafts has been reported and described in previous studies for the correction of PH after failure of the conventional herniorrhaphy. The purpose of this report is to describe a modified technique of fascia lata flap (FLP) for PH repair in dogs. Six client-owned male dogs with PH were included in the study. PH was repaired with a FLP obtained from the dog's ipsilateral thigh and sutured directly into the perineal defect. Caudal scrotal orchiectomy was performed in all dogs that were not …

An Analysis Of The Use Of Dogs In Predicting Human Toxicology And Drug Safety, Jarrod Bailey, Michelle Thew, Michael Balls

Jarrod Bailey, PhD

Dogs remain the main non-rodent species in preclinical drug development. Despite the current dearth of new drug approvals and meagre pipelines, this continues, with little supportive evidence of its value or necessity. To estimate the evidential weight provided by canine data to the probability that a new drug may be toxic to humans, we have calculated Likelihood Ratios (LRs) for an extensive dataset of 2,366 drugs with both animal and human data, including tissue-level effects and Medical Dictionary for Regulatory Activities (MedDRA) Level 1–4 biomedical observations. The resulting LRs show that the absence of toxicity in dogs provides virtually no …

Predicting Human Drug Toxicity And Safety Via Animal Tests: Can Any One Species Predict Drug Toxicity In Any Other, And Do Monkeys Help?, Jarrod Bailey, Michelle Thew, Michael Balls

Jarrod Bailey, PhD

Animals are still widely used in drug development and safety tests, despite evidence for their lack of predictive value. In this regard, we recently showed, by producing Likelihood Ratios (LRs) for an extensive data set of over 3,000 drugs with both animal and human data, that the absence of toxicity in animals provides little or virtually no evidential weight that adverse drug reactions will also be absent in humans. While our analyses suggest that the presence of toxicity in one species may sometimes add evidential weight for risk of toxicity in another, the LRs are extremely inconsistent, varying substantially for …

An Analysis Of The Use Of Animal Models In Predicting Human Toxicology And Drug Safety, Jarrod Bailey, Michelle Thew, Michael Balls

Jarrod Bailey, PhD

Animal use continues to be central to preclinical drug development, in spite of a lack of its demonstrable validity. The current nadir of new drug approvals and the drying-up of pipelines may be a direct consequence of this. To estimate the evidential weight given by animal data to the probability that a new drug may be toxic to humans, we have calculated Likelihood Ratios (LRs) for an extensive data set of 2,366 drugs, for which both animal and human data are available, including tissue-level effects and MedDRA Level 1–4 biomedical observations. This was done for three preclinical species (rat, mouse …

Evaluating The Physical Welfare Of Dogs In Commercial Breeding Facilities In The United States, Moriah J. Hurt

Open Access Theses

Despite high levels of public concern, the state of commercial breeding (CB) dog welfare is largely unknown. Therefore, this study aimed to characterize the physical welfare of dogs in CB facilities and their environment in Indiana. This study specifically aimed 1) to characterize dog foot health in CB facilities as a function of the length of time dogs were housed on a given flooring substrate, 2) to characterize visual dog body cleanliness and visual kennel cleanliness as a function of the flooring substrate, 3) to determine the efficacy of kennel cleaning procedures and 4) to characterize the dog dental and …