Life Sciences Commons^™

Pathogenicity In Chickens And Turkeys Of A 2021 United States H5n1 Highly Pathogenic Avian Influenza Clade 2.3.4.4b Wild Bird Virus Compared To Two Previous H5n8 Clade 2.3.4.4 Viruses, Mary J. Pantin-Jackwood, Erica Spackman, Christina Leyson, Sungsu Youk, Scott A. Lee, Linda M. Moon, Mia K. Torchetti, Mary L. Killian, Julianna B. Lenoch, Darrell R. Kapczynski, David E. Swayne, David L. Suarez

USDA Wildlife Services: Staff Publications

Highly pathogenic avian influenza viruses (HPAIVs) of subtype H5 of the Gs/GD/96 lineage remain a major threat to poultry due to endemicity in wild birds. H5N1 HPAIVs from this lineage were detected in 2021 in the United States (US) and since then have infected many wild and domestic birds. We evaluated the pathobiology of an early US H5N1 HPAIV (clade 2.3.4.4b, 2021) and two H5N8 HPAIVs from previous outbreaks in the US (clade 2.3.4.4c, 2014) and Europe (clade 2.3.4.4b, 2016) in chickens and turkeys. Differences in clinical signs, mean death times (MDTs), and virus transmissibility were found between chickens and …

Research Note: Association Of Single Nucleotide Polymorphism Of Akt3 With Egg Production Traits In White Muscovy Ducks (Cairina Moschata)., Semiu Folaniyi Bello, Haiping Xu, Kan Li, Lijin Guo, Siyu Zhang, Ridwan Olawale Ahmed, Endashaw Jebessa Bekele, Ming Zheng, Mingjian Xian, Bahareldin Ali Abdalla, Adeniyi Charles Adeola, Adeyinka Abiola Adetula, Raman Akinyanju Lawal, Weijian Zhu, Dexiang Zhang, Xiquan Zhang, Congliang Ji, Qinghua Nie

Faculty Research 2022

Prior studies on transcriptomes of hypothalamus and ovary revealed that AKT3 is one of the candidate genes that might affect egg production in White Muscovy ducks. The role of AKT3 in the uterus during reproductive processes cannot be overemphasized. However, functional role of this gene in the tissues and on egg production traits of Muscovy ducks remains unknown. To identify the relationship between AKT3 and egg production traits in ducks, relative expression profile was first examined prior to identifying the variants within AKT3 that may underscore egg production traits [age at first egg (AFE), number of eggs at 300 d …

Time Course Investigation Of The Dermal Leukocyte Response To Lipoteichoic Acid In Chickens, Ian Gilbert

Animal Science Undergraduate Honors Theses

Lipoteichoic acid (LTA) is component of the cell wall of Gram-positive bacteria that stimulates inflammation during bacterial infection. However, few studies have investigated the in vivo immune response to LTA, and none of the in vivo studies done have been performed in birds. For this project, the pulp (a skin-derivative) of growing feathers (GFs) of chickens were used as a test site to investigate the in vivo effects of intradermally injected LTA. In Study 1, the pulp of 12 GFs of 11-week-old Light-brown Leghorn (LBL) males were injected with 10 μL of differing concentrations of LTA (0.1, 1.0, 10, 100 …

Time Course Investigation Of The Dermal Leukocyte Response To Lipoteichoic Acid In Chickens, Ian M. Gilbert, Jossie M. Santamaria, Gisela F. Erf

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Lipoteichoic acid (LTA) is a cell-wall polymer in Gram-positive bacteria that stimulates inflammation. Few studies have investigated in vivo immune response to LTA, and none of the in vivo studies have been performed in birds. For this project, the pulp (a skin derivative) of growing feathers (GF) of chickens was used to investigate the in vivo effects of intradermally injected LTA. In Study 1, GF of chickens were injected with 10 μL of differing concentrations of LTA (0.1, 1.0, 10, 100 or 250 μg LTA/mL; 3 chickens/dose). Growing feathers were plucked before injection (0 h) and at 6, 24, 48, …

Role Of Protein Charge Density On Hepatitis B Virus Capsid Formation, Xinyu Sun, Dong Li, Zhaoshuai Wang, Panchao Yin, Rundong Hu, Rundong Hu, Hui Li, Qiao Liu, Yunyi Gao, Baiping Ren, Jie Zheng, Yinan Wei, Tianbo Liu

Chemistry Faculty Publications

The role of electrostatic interactions in the viral capsid assembly process was studied by comparing the assembly process of a truncated hepatitis B virus capsid protein Cp149 with its mutant protein D2N/D4N, which has the same conformational structure but four fewer charges per dimer. The capsid protein self-assembly was investigated under a wide range of protein surface charge densities by changing the protein concentration, buffer pH, and solution ionic strength. Lowering the protein charge density favored the capsid formation. However, lowering charge beyond a certain point resulted in capsid aggregation and precipitation. Interestingly, both the wild-type and D2N/D4N mutant displayed …

Avian Salmonella Enteritidis Serovar Enteritidis With Clpp Deletion Is Attenuated And Highly Immunogenic In Chickens, Xuhua Ran, Zhiyuan Yang, Guagnxi Shao, Xiaobo Wen, Siguo Liu, Hongbo Ni

Turkish Journal of Veterinary & Animal Sciences

Salmonella enterica subsp. enterica serovars are leading causative agents of gastroenteritis in humans and numerous animal species worldwide. The ClpP from Salmonella enterica is responsible for regulation under various stress conditions. We were interested in the effect of ClpP deletion mutation on attenuation of Salmonella enterica subsp. enterica serovar Enteritidis (SE) in chickens. Therefore, SM-6/Δ ClpP , deletion of the ClpP gene from a wild-type SE-6 (SM-6) strain, was generated using the phage &lamda; Red homologous recombination. Compared with SM-6, SM-6/&DeltaClpP represented a retarded growth rate and lower concentration in LB broth with and without 5% NaCl or lower pH …

Age-Related Changes In The Growth Performance, Meat Quality, And Oxidativeprocesses In Breast Muscles Of Three Chicken Genotypes, Monika Michalczuk, Artur Józwik, Krzysztof Damaziak, Zaneta Zdanowska-Sasiadek, Agata Marzec, Dariusz Gozdowski, Nina Strzalkowska

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine growth performance, meat quality, and oxidative changes in breast muscles depending on the genotype and age of broiler chickens. The experiment was conducted on 1080 chickens: fast-growing Cobb 500, medium-growing Hubbard JA957, and a slow-growing experimental line. Chickens from all genetic groups were reared on litter for 8 weeks, but from the 5th week of age, 15 chickens from each group were slaughtered every week. After slaughter at weeks 5, 6, 7, and 8 of chickens? lives, their muscles were sampled for chemical composition, fatty acid profile, and cholesterol. Contents of reduced …

Selecting Eimeria Tenella-Resistance Markers By Comparing Resistant And Susceptible Groups Of Jinghai Yellow Chickens, Jingjing Zhang, Yuxin Lin, Guojun Dai, Tingting An, Mingming Sun, Genxi Zhang, Kaizhou Xie, Jingyu Wang

Turkish Journal of Veterinary & Animal Sciences

Improving the genetic resistance of chickens to parasitic diseases is an alternative way to protect the poultry industry. To establish valid measurable in vivo parameters indicating coccidiosis resistance, 232 Jinghai Yellow chickens were randomly selected and orally infected with Eimeria tenella (1.5 x 104 sporulated oocysts per chicken). The resistant and susceptible chickens were classified based on their cecal lesion scores 8 days after infection. Ten plasma components, including antioxidant enzymes, interleukins (ILs), nitric oxide (NO), interferon-γ (IFN-γ), and β-carotene, were compared among the resistant, susceptible, and control chickens (uninfected with E. tenella). The NO, superoxide dismutase (SOD), IL17, and …

Influence Of Env And Long Terminal Repeat Sequences On The Tissue Tropism Of Avian Leukosis Viruses, David Brown, Harriet Robinson

David C. Brown

Adsorption and penetration of retroviruses into eucaryotic cells is mediated by retroviral envelope glycoproteins interacting with host receptors. Recombinant avian leukosis viruses (ALVs) differing only in envelope determinants that interact with host receptors for subgroup A or E ALVs have been found to have unexpectedly distinctive patterns of tissue-specific replication. Recombinants of both subgroups were highly expressed in bursal lymphocytes as well as in cultured chicken embryo fibroblasts. In contrast, the subgroup A but not subgroup E host range allowed high levels of expression in skeletal muscle, while subgroup E but not subgroup A envelope glycoproteins permitted efficient replication in …

Identification Of A Family Of Fatty Acid-Speciated Sonic Hedgehog Proteins, Whose Members Display Differential Biological Properties, Jun Long, Robert Tokhunts, William M. Old, Stephane Houel, Jezabel Rodgriguez-Blanco, Samer Singh, Neal Schilling, Anthony J. Capobianco, Natalie G. Ahn, David J. Robbins

Dartmouth Scholarship

Hedgehog (HH) proteins are proteolytically processed into a biologically active form that is covalently modified by cholesterol and palmitate. However, most studies of HH biogenesis have characterized protein from cells in which HH is overexpressed. We purified Sonic Hedgehog (SHH) from cells expressing physiologically relevant levels and showed that it was more potent than SHH isolated from overexpressing cells. Furthermore, the SHH in our preparations was modified with a diverse spectrum of fatty acids on its amino termini, and this spectrum of fatty acids varied dramatically depending on the growth conditions of the cells. The fatty acid composition of SHH …

An Hsi Fact Sheet: Avian Influenza In India, Humane Society International

HSI FACT SHEETS

Avian influenza (AI), commonly known as bird flu, typically causes little or no harm to its wild waterfowl hosts. In 2003-2004, however, nine Asian countries reported unprecedented outbreaks of high-mortality AI in domestic poultry. This viral lineage, subtype H5N1, has spread to over 60 countries, and is considered endemic in at least four.

In India, H5N1 outbreaks were first recorded in 2006, and have continued each year since, reaching a total of 86 by 2012. Millions of India’s chickens and ducks have been culled in efforts to contain and eliminate the virus. India has declared itself free of AI a …

Genetic Analysis Of Chromosomal Regions Affecting Sperm Mobility In The Chicken (Gallus Gallus Domesticus), Sharath Chandra Munnaluri

Graduate Theses and Dissertations

This project is focused on determining the contributions of particular genetic loci to low sperm mobility in the chicken. Sperm mobility has been shown to be a major determinant of male fertility in broiler breeders. A whole genome SNP scan identified chromosomal regions (QTLs) that control sperm mobility. I used microsatellites from 4 chromosomal regions ( 15.151 on chromosome 6, 12.435 on chromosome Z, 12.341 on chromosome Z, and 17.214 on chromosome Z) to determine the association of these regions with sperm mobility in experimental lines divergently selected for mobility phenotype. Determination of the genetics underlying sperm mobility will identify …

Characterization Of The Vasotocin Receptor Subtype 4 (Vt4r) In The Brain And Pituitary Gland Of The Chicken, Gallus Gallus, Rajamani Selvam

Graduate Theses and Dissertations

The present study investigated the distribution of the vasotocin subtype four receptor (VT4R) in brain and pituitary gland of the chicken, Gallus gallus. The anterior pituitary cell types associated with the VT4R were also determined. Two polyclonal antibodies were raised in rabbit against a cocktail of peptides, 15 amino acids from the amino terminal region and 17 amino acids from the carboxy terminal region of VT4R receptor. The antibody was validated utilizing the Western blot and immunocytochemistry. A single band at 47KDa utilizing membrane protein extracts of chicken brain and pituitary tissues was shown for the VT4R antibody. A peptide …

About Chickens, The Humane Society Of The United States

Agribusiness Collection

The chicken is the world’s most numerous domesticated bird, with over 52 billion farmed worldwide in 2008, rivaling the dog as the most ubiquitous domestic animal globally. These birds have fascinated scholars and researchers since the dawn of Western civilization, and recent studies are beginning to reveal the depths of their complexity and cognitive ability. According to Andrew F. Fraser, professor of veterinary surgery at Memorial University of Newfoundland, and Donald M. Broom, professor of animal welfare at University of Cambridge: “Those who have studied the behaviour of the domestic fowl in detail…, especially those who have looked at feral …

F-Actin And Myosin Ii Binding Domains In Supervillin, Yu Chen, Norio Takizawa, Jessica Crowley, Sang Oh, Cheryl Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, Mitsuo Ikebe, Elizabeth Luna

Elizabeth J. Luna

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts. A subset of these detergent-resistant membranes (DRM-H) exhibits a higher buoyant density ( approximately 1.16 g/ml) because of association with membrane skeleton proteins, including actin, myosin II, myosin 1G, fodrin, and an actin- and membrane-binding protein called supervillin (Nebl, T., Pestonjamasp, K. N., Leszyk, J. D., Crowley, J. L., Oh, S. W., and Luna, E. J. (2002) J. Biol. Chem. 277, 43399-43409). To characterize interactions among DRM-H cytoskeletal proteins, we investigated the binding partners of the novel supervillin N terminus, specifically amino acids 1-830. We …

Replacement Of Fish Meal By Meal Worm (Eisenia Foetida) In The Feed Of Broilers As An Alternative Source Of Protein, Carlos ÁNgel Cervantes Cevallos

Theses and Dissertations

This investigation was conducted in the rural community of Cuambo, located in the northeast of Imbabura province, in the Mira river basin of Salina parish, at 1530 meters above sea level and with an average temperature of 19 C. The principal objective of this investigation was to evaluate the effect of four levels of substitution of fish meal for earthworm meal in broiler chicken diets. The fieldwork occurred in two stages over the course of 20 months: A. Creation of the feed: This step lasted from the installation of an earthworm culturing area through the cultivation, harvest, and drying of …

Marek's Disease Virus (Mdv) Encodes An Interleukin-8 Homolog (Vil-8): Characterization Of The Vil-8 Protein And A Vil-8 Deletion Mutant Mdv, Mark S. Parcells, Su-Fang Lin, Robert L. Dienglewicz, Vladimir Majerciak, Dan R. Robinson, Hua-Chien Chen, Zining Wu, George R. Dubyak, Peter Brunovskis, Henry D. Hunt

Dartmouth Scholarship

Chemokines induce chemotaxis, cell migration, and inflammatory responses. We report the identification of an interleukin-8 (IL-8) homolog, termed vIL-8, encoded within the genome of Marek's disease virus (MDV). The 134-amino-acid vIL-8 shares closest homology to mammalian and avian IL-8, molecules representing the prototype CXC chemokine. The gene for vIL-8 consists of three exons which map to the BamHI-L fragment within the repeats flanking the unique long region of the MDV genome. A 0.7-kb transcript encoding vIL-8 was detected in an n-butyrate-treated, MDV-transformed T-lymphoblastoid cell line, MSB-1. This induction is essentially abolished by cycloheximide and herpesvirus DNA polymerase inhibitor phosphonoacetate, indicating …

Feasibility Of Inducing Overlap Immunologic Competence In Gallinaceous Birds With Ascardia Dissimilis And A. Galli, Julie Hamilton, Thomas A. Yazwinski

Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences

Chickens and turkeys are routinely infected with the roundworms Ascaridia galli and A. dissimilis, respectively. The current study was conducted to gather basic information on these worms and to determine whether heterologous infections (chicken worms in turkeys and turkey worms in chickens) would be successful. Chickens and turkeys were obtained at day of hatch, brooded to 7 days of age, and placed in pens (25/pen) according to infection as received at 7 days of age: homologous, heterologous and control (no infection). Bird weights, mortalities, and feed efficiencies were monitored for 3 weeks postinfection, at which time all birds were killed …

Pathological And Biochemical Studies On "Fatty Liver And Kidney Syndrome" In White Broiler Chickens Fed With Ration Including Rendering Oil, Erkan Karadaş, Harun Özer, Enver Beytut, Necmi Özdemi̇r

Turkish Journal of Veterinary & Animal Sciences

The "Fatty Liver and Kidney Syndrome" (FLKS) was induced experimentally in white broiler chickens. Eighty chickens of ten-day-old were divided into equally five groups. These chickens were fed with a daily provided commercial ration containing poultry rendering oil in a ratio of 0% in group I (control group), 5% in group II, 10% in group III, 15% in group IV and 20% in group V for eight weeks. Total mortality was 18.75%. Although group II chickens did not show significant clinical, morphological and biochemical findings, these findings were found to be significant in groups III, IV and V. In these …

The Coccidia Of The Gallinaceous Birds In Azerbaijan, Musa Musaev, Gamida Gaibova, Galiba Ismailova, Farida Alieva, Naila Iskenderova

Turkish Journal of Veterinary & Animal Sciences

In this study the data on the coccidia of gallinaceous birds in Azerbaijan were presented. Chickens and pheasants have already been examined earlier concerning the presence of Eimeria and Isospora. Peacocks and quails are studied in this aspect for the first time. The examination of the gallinaceous birds concerning the presence of crytosporidia oocysts has been also carried out for the first time. The results obtained are indicative of the presence of a few coccidian species in the gallinaceous birds which have not been detected in Azerbaijan before.

Effect Of Dietary Aluminum Sulfate On Calcium And Phosphorus Metabolism Of Broiler Chicks, Akmed S. Hussein, Austin H. Cantor, Thomas H. Johnson, Robert A. Yokel

Animal and Food Sciences Faculty Publications

The effect of dietary aluminum sulfate on Ca and P metabolism was studied using 1-day-oldmale broiler chicks. In Experiment 1, practical diets providing .90% Ca plus .45% available P (P_av), .90% Ca plus .78% P_av, 1.80% Ca plus .45% P_av, or 1.80% Ca plus .90% P_av were fed with 0 or .392% Al as aluminum sulfate for 21 days. The control diet (.90% Caplus .45% P_av) without added A1 was fed to all chicks during Days 22 to 49. In general, Al significantly (Pi), tibia breaking strength, tibia weight, percentage of …

Relationship Of Dietary Aluminum, Phosphorus, And Calcium To Phosphorus And Calcium Metabolism And Growth Performance Of Broiler Chicks, Akmed S. Hussein, Austin H. Cantor, Thomas H. Johnson, Robert A. Yokel

Animal and Food Sciences Faculty Publications

Dietary treatments providing three levels of added Al (0, .196, or .392%) as aluminum sulfate and of available phosphorus (P_av) (.45, .68, or .78%) in a factorial arrangement were administered to day-old chicks in Experiment 1. Plasma inorganic phosphorus (P_i) was significantly (P < .05) elevated by increasing P_av and was decreased by Al. Body weight gain, feed intake, and the gain:feed ratio at Day 21 were significantly decreased by increased concentrations of Al, but were unaffected by the P_av concentrations. Decreases of 39 and 73% in weight gain and of 34 and 66% in feed intake resulted from feeding …

Hints On Chicken Rearing, P Smetana

Journal of the Department of Agriculture, Western Australia, Series 4

For the next few months poultry farmers will once again be concerned with rearing chickens.

A good brooder, ample space, good sanitation, plenty of water and a balanced feed are still the essentials of successful chicken raising, says P. Smetana, B.Sc. (Agric), Acting Officer-in-Charge of the Poultry Branch.