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Development Of Multiplex Rt-Pcr For Detection And Differentiationof Foot-And-Mouth Disease Virus O And A Serotypes In Turkey, Beyhan Sareyyüpoğlu, İbrahi̇m Burgu
Development Of Multiplex Rt-Pcr For Detection And Differentiationof Foot-And-Mouth Disease Virus O And A Serotypes In Turkey, Beyhan Sareyyüpoğlu, İbrahi̇m Burgu
Turkish Journal of Veterinary & Animal Sciences
In this study, a two-step multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed and evaluated using designed primers for the simultaneous detection and differentiation of Turkish foot-and-mouth disease virus (FMDV) serotypes A and O from clinical samples. In the method, a mix of one universal reverse primer designed from the 2B gene and two serotype-specific forward primers designed from the hypervariable regions of the capsid VP1-1D coding gene of FMDV were used. Totally, 272 FMDV-infected samples collected between 2006 and 2008 constituted the material of the study. mRT-PCR was compared to ELISA in terms of positivity percentage (%) and the …
Detection And Economic Impact Related To Bovine Respiratory Disease, Shrink, Andtraveling Distance In Feedlot Cattle In Northwest Mexico, Jose Luis Rodríguez-Castillo, Gilberto Lopez Valencia, Francisco Javier Monge Navarro, Gerado Enrique Medina Basulto, Sawako Hori-Oshima, Sergio Arturo Cueto-González, Alfonso De La Mora-Valle, Luis Mario Muñoz-Del Real, Luis Tinoco-Gracia, Tomas Benjamin Rentería-Evangelista
Detection And Economic Impact Related To Bovine Respiratory Disease, Shrink, Andtraveling Distance In Feedlot Cattle In Northwest Mexico, Jose Luis Rodríguez-Castillo, Gilberto Lopez Valencia, Francisco Javier Monge Navarro, Gerado Enrique Medina Basulto, Sawako Hori-Oshima, Sergio Arturo Cueto-González, Alfonso De La Mora-Valle, Luis Mario Muñoz-Del Real, Luis Tinoco-Gracia, Tomas Benjamin Rentería-Evangelista
Turkish Journal of Veterinary & Animal Sciences
Bovine respiratory disease (BRD) is the main reason for economic loss in feedlots. A cross-sectional study was conducted in order to detect the pathogens involved in BRD. A total of 88 animals with respiratory signs were sampled for diagnosis using PCR. The detected pathogens were bovine respiratory syncytial virus (80.6%), Mannheimia haemolytica (79.5%), Pasteurella multocida (68.1%), parainfluenza 3 virus (23.8%), bovine herpes virus-1 (20.4%), and bovine viral diarrhea virus (11.3%). The average number of treatments applied per animal was 1.3 with an estimated treatment cost of 16 USD per treatment/animal. Animals receiving more than one treatment averaged costs exceeding 38 …
Development Of Leptospiral Outer Membrane Protein-Based Dot-Elisa Assays And Immunoprofiling For Diagnosis Of Acute Canine Leptospirosis, Vamshi Krishna Sriram, Siju Joseph, Ambily Ramachandran, Mini Mangattumuruppel
Development Of Leptospiral Outer Membrane Protein-Based Dot-Elisa Assays And Immunoprofiling For Diagnosis Of Acute Canine Leptospirosis, Vamshi Krishna Sriram, Siju Joseph, Ambily Ramachandran, Mini Mangattumuruppel
Turkish Journal of Veterinary & Animal Sciences
The present study was carried out to evaluate outer membrane protein (OMP)-based relatively simple dot-ELISA assays for the diagnosis of acute canine leptospirosis and to identify the differential expression of leptospiral OMPs for identifying the markers of acute infection, if any. Among 91 canine serum samples screened, 63 samples were found to be positive by MAT. All the samples were tested by OMP-based IgM and IgG dot ELISAs. The sensitivities of IgM and IgG dot ELISA in comparison to MAT (titer: ≥1:400) were found to be 100%, whereas the specificities were found to be 89.71% and 45.31%, respectively. IgM and …