Life Sciences Commons^™

Determining The Protective Effect Of A Boron Adjuvanted Vaccine With An Experimental Infection Model In Mice, Zafer Sayin, Ali̇ Uslu, Özgür Özdemi̇r, Abdullah Başoğlu, Asli Balevi̇, Uçkun Sai̇t Uçan, Zeki̇ Aras, Osman Ergani̇ş

Turkish Journal of Veterinary & Animal Sciences

Boron is a required trace element for plants, humans, and animals and it has significant influence over biological functions and the immune system. Vaccines have aided humans in the control of many contagious diseases around the world. The success of global immunization campaigns may be possible with the development of effective and low-cost vaccines and adjuvants. For this reason, the present study aimed to determine the adjuvant activity of easily accessible boron compounds. In this study, six different vaccines were prepared, including inactive Staphylococcus aureus bacteria, boric acid, aluminum hydroxide, montanide ISA 50, and ISA 206, ISA 50 + boric …

Sars-Cov-2: Tale Of A Microscopic Murderer, Josiah P. Garner

Quest

Independent Study

Research in progress for BIOL1406: Biology for Science Majors I

Faculty Mentor: Amina Tassa, Ph.D.

I am delighted to introduce Josiah Garner’s “SARS-CoV-2: Tale of a Microscopic Murderer.” This independent study assignment explores the impact of a novel, deadly, and worldwide virus. The assignment also examines the fast development of vaccines to control the spread and reduce the symptoms of the virus.

Josiah’s paper focuses on the early history of the emergence of COVID-19, the world response, and vaccine development. He demonstrates critical thinking skills and effectively utilizes various research methods to obtain and communicate his information. Josiah …

The Determinants Of Covid-19 Vaccine Acceptance In Sumatra, Cindy Sidarta, Andree Kurniawan, Nata P H Lugito, Jeremia Imanuel Siregar, Veli Sungono, Rivaldo Steven Heriyanto, Novia Lauren Sieto, Devina Adella Halim, Claudia Jodhinata, Saraswati Anindita Rizki, Terry Devita Sinaga, Chintya Marcella, Felix Wijovi, Billy Susanto, Elizabeth Marcella, Johan Wibowo, Jonathan Juniard Anurantha, Michelle Imanuelly, Michele Indrawan, Nadia Khoirunnisa Heryadi

Kesmas

In light of the current coronavirus disease 2019 (COVID-19) vaccination programs being implemented worldwide, this study aimed to evaluate the COVID-19 vaccine acceptance survey in Indonesia conducted by the World Health Organization (WHO), the Ministry of Health of the Republic of Indonesia, the National Immunization Technical Advisory Group (NITAG), and the United Nations Children's Fund (UNICEF), published in November 2020. It was found that Sumatra Island having lower COVID-19 vaccine acceptance rates, with Aceh Province displaying the lowest level of vaccine acceptance. Thus, a cross-sectional study was conducted, and a logistic regression analysis was used to identify the factors affecting …

Immune Response To An Alum Precipitated Haemorrhagic Septicaemia Vaccine In Buffaloes At A Semiorganized Farm Of Madhya Pradesh In India, Sachin Audarya, Naresh Kakker, Rakesh Sharda, Daljeet Chhabra

Turkish Journal of Veterinary & Animal Sciences

Haemorrhagic septicaemia (HS) is one of the economically most important bacterial diseases of large ruminants. In the present investigation, a total of 156 buffaloes (Murrah, Jafarabadi, and Bhadawari breeds) at a semiorganized farm after administration of HS alum precipitated killed vaccine were screened serologically for the presence of anti-HS antibodies by single dilution indirect enzyme linked immunosorbent assay (iELISA). Out of 156 buffaloes tested for presence of anti-HS antibodies, a greater proportion of buffalo (67.94%) had protective level of anti-HS antibodies even after 90th day post vaccination.

The Deadly Influenza Virus And Its Changing Forms, Kayce Boggess

D.U.Quark

The influenza virus has plagued humans for centuries. Recently antiviral medications, which shorten the duration of the flu, have been introduced into society. These medications along with vaccinations, which try to give the body immunity before the virus strikes, help to stop the flu before it attacks the host. The virus, however, replicates using host cells and can slightly change itself with each replication, which over time could lead to a strain immune to the current antiviral medication and vaccines. However, using more preventative measures could help slow the changing strains of the flu virus. Using vaccines to stop the …

Avian Salmonella Enteritidis Serovar Enteritidis With Clpp Deletion Is Attenuated And Highly Immunogenic In Chickens, Xuhua Ran, Zhiyuan Yang, Guagnxi Shao, Xiaobo Wen, Siguo Liu, Hongbo Ni

Turkish Journal of Veterinary & Animal Sciences

Salmonella enterica subsp. enterica serovars are leading causative agents of gastroenteritis in humans and numerous animal species worldwide. The ClpP from Salmonella enterica is responsible for regulation under various stress conditions. We were interested in the effect of ClpP deletion mutation on attenuation of Salmonella enterica subsp. enterica serovar Enteritidis (SE) in chickens. Therefore, SM-6/Δ ClpP , deletion of the ClpP gene from a wild-type SE-6 (SM-6) strain, was generated using the phage &lamda; Red homologous recombination. Compared with SM-6, SM-6/&DeltaClpP represented a retarded growth rate and lower concentration in LB broth with and without 5% NaCl or lower pH …

Genome Diversity And Molecular Detection Of Prrs Field Strains And Vaccine Strains, And Pcv3 And Pcv2 Strains, Y. Wang, F. Yuan, X. Liu, W. Zheng, H. Zhang, J. Zhang, K. Yoon, L. Peddireddi, Y. Fang, G. Anderson, J. Bai

Kansas Agricultural Experiment Station Research Reports

Molecular diagnosis of porcine reproductive and respiratory syndrome virus (PRRS) and porcine circo virus (PCV) are challenging due to high genetic diversity in the viral genomes. Differentiating PRRS vaccine strains is even more challenging and is currently done by DNA sequencing, which is expensive and time-consuming. A multiplexed system (Luminex) allowing multiple detection targets in the same reaction is available. However, this system is not fully developed for common swine pathogens. Therefore, an assay was built to detect the majority of field PRRS strains by using different pairs of primers, and at the same time, to provide differentiation of the …

Purification Of Avian Influenza Virus (H9n2) From Allantoic Fluidby Size-Exclusion Chromatography, Ali Nazari Shirvan, Maedeh Samianifard, Naser Ghodsian

Turkish Journal of Veterinary & Animal Sciences

Avian influenza viruses represent a rising threat of pandemic influenza. Allantoic fluid including influenza virus H9N2 was run on downstream processing to purify the virus. To do this, allantoic fluid was clarified and concentrated. It was applied to size exclusion chromatography. Different parameters such as ovalbumin, protein content, and hemagglutinin activity were determined to confirm the purity. The results may suggest that this method is quite efficient for achieving a purified H9N2 influenza virus and further reveal that size-exclusion chromatography is a practical method for purifying avian influenza viruses. In conclusion, this procedure can be used in vaccine production and …

Comparative Study Between Formalin-Killed Vaccine And Developed Gammairradiation Vaccine Against Mannheimia Haemolytica In Rabbits, Sahar Ahmed, Basem Ahmed, Ghada Mahmoud, Waleed Nemr, Emam Abdel Rahim

Turkish Journal of Veterinary & Animal Sciences

Mannheimia haemolytica is responsible for considerable economic losses to sheep, goats, and cattle and other livestock industries in Egypt. This study aimed to evaluate the effectiveness of a newly developed gamma irradiation vaccine against Mannheimia haemolytica in comparison to a formalin-killed vaccine. Three groups of rabbits were used in this study. Group 1 animals were inoculated with 4 × 109 bacterial cells per dose of the formalin-killed vaccine. Group 2 was inoculated with 2 × 109 bacterial cells per dose of gamma-irradiated vaccine. Group 3 (control group) was injected with 2 mL of sterile PBS. The vaccines were injected subcutaneously …

Efficacy Of Experimental Inactivated And Live Rhodococcus Equivaccines For Thoroughbred Arabian Mares In Mice, Osman Ergani̇ş, Hasan Hüseyi̇n Hadi̇mli̇, Zafer Sayin, Asli Sakmanoğlu, Yasemi̇n Pinarkara, Özgür Özdemi̇r

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to determine the efficacy of inactive Rhodococcus equi vaccine candidates included that bacterin+aluminum hydroxide (Al(OH)3), bacterin+VapA+(Al(OH)3), bacterin+Montanide IMS 3012 (IMS), bacterin+VapA+IMS, and live vaccine using mice as a model. The efficacy of vaccine was evaluated according to clinical findings, humoral and cellular immunity (levels of INF-g and IL-4), and results of microbiological culture from internal organs in dead or sacrificed mice. Inactive R. Equi vaccines were subcutaneously administered to mice three times at 15-day intervals and live vaccine was intraperitoneally injected once. Fifteen days after the last vaccination, aerosol challenges were carried out with …

Human Papillomavirus And The Gardasil Vaccine: Medicalization And The Gendering Of Bodies And Bodily Risk, Lauren Camara

The Partisan

No abstract provided.

Comparative Studies On The Efficacy Of Different Vaccination Methods In The Treatment Of Bovine Papillomatosis, İbrahi̇m Çi̇mtay, Hali̇l Selçuk Bi̇ri̇ci̇k, Oktay Keski̇n, Zeli̇ha Füsun Baba

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare the efficacy of autogenous, species-specific and herd-specific vaccination in the treatment of bovine papillomatosis. This study was performed on 24 female Holstein cattle aged between 8 and 17 months in the same herd. The animals were divided into three vaccination groups (eight cattle in each group) as autogenous, species-specific and herd-specific. A dose of 10 ml per animal was inoculated subcutaneously every 10 days, for a total of three times. The recovery status of the animals was checked for 3 months at 15-day intervals. In this study, all the animals fully recovered …

Comparison Of Autogenous Vaccine And Autohaemotherapy Administrations In The Treatment Of Bovine Papillomatosis, Hali̇l Selçuk Bi̇ri̇ci̇k, Oktay Keski̇n, İbrahi̇m Çi̇mtay, Zeli̇ha Füsun Baba

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to compare the efficacy of autogenous vaccine, autohaemotherapy and autogenous vaccine-autohaemotherapy combinations in the treatment of bovine papillomatosis. The study was performed on 27 cattle aged between 1 and 2.5 years. The animals were divided into three groups (nine in each group) as autogenous vaccine, autohaemotherapy and autogenous vaccine-autohaemotherapy combinations. Doses of 10 ml per animal were injected subcutaneously at weekly intervals, a total of four times in the autogenous vaccine group. In the autohaemotherapy group, blood withdrawn from the jugular vein was administered at three-day intervals, a total of four times, in 40 …

Vaccine Development From Serotypes O1, O2 And O78 Of Escherichia Coli Against Avian Colibacillosis: Layer Chickens, Osman Ergani̇ş, H. H. Hadi̇mli̇, Hasan Solmaz

Turkish Journal of Veterinary & Animal Sciences

A trivalent Escherichia coli vaccine (TECA) was prepared from serotypes O1, O2 and O78 and one series of TECA was combined with an inactivated Newcastle Disease vaccine. TECA and combined vaccine were experimentally investigated for immunogenic effects in pullets and hens. This study was carried out at two stages consisting of rearing and laying periods: (1) A number of fourty chicks aged 15 days were subcutaneously vaccinated with 0.2 ml TECA . A similar group served as non-vaccinated controls. Half of the vaccinated group were revaccinated with 0.5 ml TECA at 71 days of age. The vaccinated and nonvaccinated pullets …

Development Of Vaccines To Meet Public Health Needs: Incentives And Obstacles, Phillip K. Russell

RISK: Health, Safety & Environment (1990-2002)

Dr. Russell explains how such matters as high 'costs of regulation, lack of an effective plan for delivery (particularly abroad) and politics can interfere with providing globally needed vaccines.

The Quest For An Aids Vaccine, Robert T. Schooley

New England Journal of Public Policy

More than fifty thousand cases of AIDS have been reported in the United States since the disease wasfirst described in 1981. Many times this number of people are infected with human immunodeficiency virus (HIV), which has been identified as the agent responsible for the illness. The seriousness of the disease, coupled with the relatively rapid spread of HIV, has fueled the effort for development of an effective vaccine.

Much is now known about the life cycle of the virus, and about its structural components. This information, and information about methods of transmission of the virus, form the basis for a …