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A Retrospective Study Of Borrelia Burgdorferi Antibodies In Dogs In Minnesota, Aneela Durrani, Sagar Goyal
A Retrospective Study Of Borrelia Burgdorferi Antibodies In Dogs In Minnesota, Aneela Durrani, Sagar Goyal
Turkish Journal of Veterinary & Animal Sciences
In order to determine the seroprevalence of Borrelia burgdorferi antibodies in suspected dogs in Minnesota, the present study examines the database of the Veterinary Diagnostic Laboratory at the University of Minnesota over a 10 year period (2001-June 2010). A total of 1081 serum samples from dogs suspected of having borreliosis were submitted by 112 private veterinary clinics. The samples were tested using an indirect fluorescent antibody (IFA) test. Samples with titers of =>1:320 were considered positive. The rate of seroprevalence of antibodies was 88%. The increase in the rate of yearly seroprevalence of Borrelia burgdorferi antibodies in dogs was …
A Loop-Mediated Isothermal Amplification (Lamp) Assay For Rapid Detection Of Anaplasma Phagocytophilum Infection In Dogs, Chungchen Lee, Yungchang Lin, Chauloong Tsang, Yangtsung Chung
A Loop-Mediated Isothermal Amplification (Lamp) Assay For Rapid Detection Of Anaplasma Phagocytophilum Infection In Dogs, Chungchen Lee, Yungchang Lin, Chauloong Tsang, Yangtsung Chung
Turkish Journal of Veterinary & Animal Sciences
A simple and rapid diagnostic method of Anaplasma phagocytophilum infection was established, employing the loop-mediated isothermal amplification (LAMP) reaction. With 1 set of 4 specific primers targeting the citrate synthase (gltA) gene of A. phagocytophilum, the LAMP assay rapidly amplified the target DNA sequence in a single tube in 1 h, requiring only a water bath for reaction. When visualized by gel electrophoresis, the LAMP products appeared as a ladder-like pattern, with many bands of different sizes from 180 base pairs up to the loading well. The results obtained from testing 32 clinical blood samples of dogs demonstrated that the …