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Determination Of Virulence Genes And Antibiotic Resistance Profiles Of Streptococcus Agalactiae Isolated From Buffalo Milk, Nevi̇n Tuzcu, Hasan Hüseyi̇n Hadi̇mli̇, Beatri̇z Padron
Determination Of Virulence Genes And Antibiotic Resistance Profiles Of Streptococcus Agalactiae Isolated From Buffalo Milk, Nevi̇n Tuzcu, Hasan Hüseyi̇n Hadi̇mli̇, Beatri̇z Padron
Turkish Journal of Veterinary & Animal Sciences
Mastitis is a contagious disease that causes significant milk loss and serious economic damage in dairy animals. Streptococcus agalactiae causes clinical and subclinical mastitis in animals and infections in humans, especially adults, and newborns. This study aimed to confirm the identification of S. agalactiae isolates by PCR in buffalo milk; to determine the antibiotic resistance and virulence genes by multiplex PCR. The material of this study consisted of 30 (12%) S. agalactiae strains identified by phenotypic, biochemical, and PCR methods (rRNA-16S) from 250 isolates with bacterial growth from milk samples of buffaloes with mastitis. In addition, the Kirby-Bauer disc diffusion …
Prevalence And Antibiotic Resistance Profiles Of Salmonella Spp. In Chicken Meat, Rabi̇a Telsaç, Rabi̇a Mehtap Tuncay
Prevalence And Antibiotic Resistance Profiles Of Salmonella Spp. In Chicken Meat, Rabi̇a Telsaç, Rabi̇a Mehtap Tuncay
Turkish Journal of Veterinary & Animal Sciences
This study aimed to determine the Salmonella spp. presence in 100 chicken meat samples (25 breasts and 25 drumsticks with skin and 25 breasts and 25 drumsticks without skin) that were collected between February and April, 2021, in Van, Türkiye and investigate the antibiotic resistance profiles of the isolates. The results of the cultivation and PCR analysis revealed that a total of 17 samples comprising four drumstick with skin (16%), five drumstick without skin and breast without skin (20%), and three breast with skin (12%) samples were positive for Salmonella spp. The analysis of 17 chicken meat samples yielded a …
Antibiotic Susceptibility And Phylogenetic Analyses For The Origins And Serotypes Of Listeria Monocytogenes Strains Isolated From Ice Cream And Cream Cakes, Orkun Babacan
Turkish Journal of Veterinary & Animal Sciences
Listeria monocytogenes is a zoonotic bacterium which also infects humans. The aim of this study was to isolate this organism from cream cakes and ice cream and obtain 16S rRNA and hlyA gene sequences from isolates in order to perform phylogenetic analyses. Serotypes and antibiotic susceptibility were also determined. The cream cake and ice cream samples were examined for L. monocytogenes according to ISO 11290-1 and using the mini Vidas LMO 2 kit procedure. Antibiotic susceptibilities were investigated using the disc diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and the Clinical and Laboratory Standards Institute …
Isolation Of Multidrug Resistant Coliforms And Their Bacteriophages From Swine Slurry, Adrian Augustyniak, Bartlomiej Grygorcewicz, Pawe? Nawrotek
Isolation Of Multidrug Resistant Coliforms And Their Bacteriophages From Swine Slurry, Adrian Augustyniak, Bartlomiej Grygorcewicz, Pawe? Nawrotek
Turkish Journal of Veterinary & Animal Sciences
Slurry management is a contemporary agricultural problem. Although liquid manure is a valuable fertiliser, it may pose a microbiological threat due to spreading of bacteria resistant to various antibiotics. Fermentation has been proposed as a solution to this problem, although it does not fully eliminate the antibiotic-resistant strains. The presence of gram-negative rods resistant to antimicrobials may cause an uncontrolled transmission of resistance factors and genes in the natural environment. Therefore, a biocontrol system is necessary to prevent this phenomenon. The aim of this study was to detect multidrug-resistant (MDR) bacteria and their lytic bacteriophages in raw swine slurry. For …
Antibiotic Resistance Profile Of Enterococcus Faecium Andenterococcus Faecalis Isolated From Broiler Cloacal Samples, Ni̇lgün Ünal, Şi̇nasi̇ Aşkar, Murat Yildirim
Antibiotic Resistance Profile Of Enterococcus Faecium Andenterococcus Faecalis Isolated From Broiler Cloacal Samples, Ni̇lgün Ünal, Şi̇nasi̇ Aşkar, Murat Yildirim
Turkish Journal of Veterinary & Animal Sciences
The present study was performed to isolate and identify Enterococcus spp. from broiler cloacal samples to species level, to determine their resistance patterns to various antibiotics, and to detect vancomycin resistance genes. Cloacal samples of broilers collected from slaughterhouses were inoculated in Slanetz and Bartley agars with and without vancomycin (6 μg/mL). Antibiotic resistance/susceptibility testing of the isolated and identified enterococci was performed by using the disk diffusion test. Multiplex PCR was used to identify the species and to detect vancomycin resistance genes. The majority of the isolated enterococci was Enterococcus faecium (60.43%, n = 142) and Enterococcus faecalis (33.62%, …
Antimicrobial Resistance Profiles And Virulence Factors Of Escherichia Colio157 Collected From A Poultry Processing Plant, Harun Hizlisoy, Serhat Al, Nurhan Ertaş Onmaz, Yeli̇z Yildirim, Zafer Gönülalan, Kadi̇r Semi̇h Gümüşsoy
Antimicrobial Resistance Profiles And Virulence Factors Of Escherichia Colio157 Collected From A Poultry Processing Plant, Harun Hizlisoy, Serhat Al, Nurhan Ertaş Onmaz, Yeli̇z Yildirim, Zafer Gönülalan, Kadi̇r Semi̇h Gümüşsoy
Turkish Journal of Veterinary & Animal Sciences
The aims of this study were to investigate the presence of Escherichia coli O157 and to determine the antibiotic susceptibility of isolates in different poultry-originated materials. In total 300 samples of chicken carcass swabs (n = 50), chicken feces (n = 50), gizzard (n = 50), liver (n = 50), water in the final scald tank (n = 50), and equipment such as tables, conveyers, and knives (n = 50) were used as materials. Overall, 45 E. coli isolates were obtained from the 300 poultry samples. However, 13 (4.3%) of the samples were identified as E. coli O157 including 3 …
Detection Of The Contamination Sources Of Listeria Monocytogenes In Pickled White Cheese Production Process Line And Genotyping With The Pulsed-Field Gel Electrophoresis Method, Ni̇hat Telli̇, Ahmet Güner, Ferda Soyer Dönmez, Özgün Öykü Özdemi̇r
Detection Of The Contamination Sources Of Listeria Monocytogenes In Pickled White Cheese Production Process Line And Genotyping With The Pulsed-Field Gel Electrophoresis Method, Ni̇hat Telli̇, Ahmet Güner, Ferda Soyer Dönmez, Özgün Öykü Özdemi̇r
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the contamination sources, serotyping profiles, and antibiotic resistance patterns of Listeria monocytogenes isolated during the production of pickled white cheese. The genetic-relatedness of the isolates to EGD SLCC (5835) (1/2a, lineage II) and ATCC (13932) (4b, lineage I) reference strains was also determined with pulsed-field gel electrophoresis (PFGE) as a result of digestions with AscI and ApaI enzymes. Samples were collected from 16 different points in the production process of 4 different plants at 3 different times. Among the 192 samples examined, 17 (8.85%) were determined to be contaminated with Listeria spp. Three isolates …
Occurrence And Antimicrobial Resistance Of Salmonella Enterica Subsp. Enterica Serovars Typhimurium, Enteritidis, And Typhi Isolated From Chicken Eggs And Poultry Products, Serhat Al, Harun Hizlisoy, Nurhan Ertaş Onmaz, Yeli̇z Yildirim, Zafer Gönülalan
Occurrence And Antimicrobial Resistance Of Salmonella Enterica Subsp. Enterica Serovars Typhimurium, Enteritidis, And Typhi Isolated From Chicken Eggs And Poultry Products, Serhat Al, Harun Hizlisoy, Nurhan Ertaş Onmaz, Yeli̇z Yildirim, Zafer Gönülalan
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to detect the occurrence and antimicrobial resistance of Salmonella enterica subsp. enterica serovars Typhimurium, Enteritidis, and Typhi in 252 samples including 100 chicken eggs, 60 processed poultry products (20 nuggets, 20 salami samples, 20 sausages), and 92 poultry giblets (50 livers and 42 gizzards). Salmonella spp. and serovars were identified by PCR. The antimicrobial susceptibility testing was performed by disk diffusion method. Forty-seven (31%) of 152 poultry products and 5 (5%) of 100 egg samples were positive for Salmonella spp. Salmonella spp. was positive in 3.3%, 5%, 18%, and 27% of chicken nugget, sausage, gizzard, …
Prevalence Of Blaz Gene And Other Virulence Genes In Penicillin-Resistant Staphylococcus Aureus Isolated From Bovine Mastitis Cases In Gansu, China, Feng Yang, Qi Wang, Xurong Wang, Ling Wang, Min Xiao, Xinpu Li, Jinyin Luo, Shidong Zhang, Hongsheng Li
Prevalence Of Blaz Gene And Other Virulence Genes In Penicillin-Resistant Staphylococcus Aureus Isolated From Bovine Mastitis Cases In Gansu, China, Feng Yang, Qi Wang, Xurong Wang, Ling Wang, Min Xiao, Xinpu Li, Jinyin Luo, Shidong Zhang, Hongsheng Li
Turkish Journal of Veterinary & Animal Sciences
Staphylococcus aureus is a major etiological agent of bovine mastitis worldwide. In this study, 37 strains of S. Aureus resistant to penicillin were isolated from bovine mastitis cases in Gansu Province for investigating blaZ and virulence-related genes, including tst, eta, etb, lukPV, lukED, lukM, hla, hlb, hld, and edin. Antibiotic resistance was based on disk diffusion method and blaZ and virulence-associated genes were studied by polymerase chain reaction. Penicillin resistance gene blaZ was detected in 35/37 (94.6%) of penicillin-resistant S. Aureus isolates. tst, lukPV, lukED, hla, hlb, and hld were observed in 5.4%, 2.7%, 89.2%, 70.3%, 73.0%, and 70.3% of …
Listeria Monocytogenes In Mussels (Mytilus Galloprovincialis) Harvested From North Aegean Coastal Area, Nikolaos Soultos, Eleni Iossifidou, Amin Ambrahim, Evdoxios Psomas, Ioannis Tzavaras, Demetrios Koutsopoulos, Thomai Lazou
Listeria Monocytogenes In Mussels (Mytilus Galloprovincialis) Harvested From North Aegean Coastal Area, Nikolaos Soultos, Eleni Iossifidou, Amin Ambrahim, Evdoxios Psomas, Ioannis Tzavaras, Demetrios Koutsopoulos, Thomai Lazou
Turkish Journal of Veterinary & Animal Sciences
One hundred and two samples of mussels (Mytilus galloprovincialis), harvested from approved shellfish coastal water in northern Greece, were screened for the presence and antimicrobial resistance of Listeria monocytogenes. Listeria spp. were isolated according to International Organization for Standardization method 11290-1: 1996/FDAM 1: 2004(E) and identified using a multiplex polymerase chain reaction (PCR) system. The serovar identity of L. monocytogenes isolates was also determined with a multiplex PCR assay. The antimicrobial profile of the isolates was determined by the disk diffusion method. Listeria spp. were present in 8 of 102 samples tested (8%) and only 1 (1%) yielded L. monocytogenes. …
Phenotypic And Genotypic Characterization Of Antibiotic-Resistant Soil And Manure Bacteria Adjacent To Swine Production Facilities, Meli̇ke Eki̇zoğlu, Satoshi Koike, Ivan Krapac, Mahmut Nedi̇m Sultan, Roderick Mackie
Phenotypic And Genotypic Characterization Of Antibiotic-Resistant Soil And Manure Bacteria Adjacent To Swine Production Facilities, Meli̇ke Eki̇zoğlu, Satoshi Koike, Ivan Krapac, Mahmut Nedi̇m Sultan, Roderick Mackie
Turkish Journal of Veterinary & Animal Sciences
This study aimed to identify phenotypic and genotypic resistance profiles of bacteria isolated from soil and manure samples obtained from a farm where tetracycline and tylosin were used extensively. Samples were collected from the manure and soil, before and after manure application. All of the 151 bacteria were identified based on BLAST results. Minimum inhibitory concentrations were determined by agar dilution and E-test methods. Class-specific primers were used to amplify 6 erythromycin and 7 tetracycline resistance (Tc^r) genes for 46 tetracycline- and 18 erythromycin-resistant isolates. Tc^r gene sequence identity between different species of isolates was identified to investigate the dissemination …
Prevalence And Antibiotic Resistance Of Listeria Spp. Isolated From Ready-To-Eat Foods In Ankara, Ufuk Tansel Şi̇reli̇, Ali̇ Gücükoğlu
Prevalence And Antibiotic Resistance Of Listeria Spp. Isolated From Ready-To-Eat Foods In Ankara, Ufuk Tansel Şi̇reli̇, Ali̇ Gücükoğlu
Turkish Journal of Veterinary & Animal Sciences
In this study the presence of Listeria spp. is tested in 100 ready-to-eat food samples purchased from different stores and traditional food shops in the province of Ankara. The tested materials were 20 each of the following: mayonnaise based salad, kadınbudu köfte (fried meatball), fried liver, rice stuffed mussel, and green salad. Microbiological analyzes showed that 13 of 100 salad samples (13%) were contaminated with Listeria spp. while 10 of 100 salad samples (10%) were contaminated with Listeria monocytogenes. Among these, 4 of 20 mayonnaise based salad samples (20%), 1 of 20 fried liver (5%), 1 of 20 rice stuffed …
Verotoxin Production In Strains Of Escherichia Coli Isolated From Cattle And Sheep, And Their Resistance To Antibiotics, Altan Aksoy, Murat Yildirim, Bi̇rgül Kaçmaz, Teoman Zafer Apan, J. Sedef Göçmen
Verotoxin Production In Strains Of Escherichia Coli Isolated From Cattle And Sheep, And Their Resistance To Antibiotics, Altan Aksoy, Murat Yildirim, Bi̇rgül Kaçmaz, Teoman Zafer Apan, J. Sedef Göçmen
Turkish Journal of Veterinary & Animal Sciences
In this study, 130 Escherichia coli strains isolated from 306 cattle and sheep fecal samples were studied for their resistance to 10 antibiotics, verotoxin production, and hemolyzation. The antibiotic resistance rates of the E. coli strains were as follows: tetracycline, 51.6%; streptomycin, 24.2%; ampicillin, 13.1%; amoxicillin/clavulanic acid, 5.2%; gentamycin, 4.6%; ciprofloxacin, 4.6%; trimethoprim-sulfamethoxazole, 4.3%; cefotaxime, 0.7%. None of the strains were resistant to cefepime or ceftazidime. Of all the antibiotics tested, only resistance to streptomycin was higher in the strains isolated from cattle than in the sheep strains (P = 0.043). The evaluation of the cattle strains, based on gender …
Antibiotic Resistance And Plasmid Dna Contents Of Streptococcus Thermophilus Strains Isolated From Turkish Yogurts, Belma Aslim, Yavuz Beyatli
Antibiotic Resistance And Plasmid Dna Contents Of Streptococcus Thermophilus Strains Isolated From Turkish Yogurts, Belma Aslim, Yavuz Beyatli
Turkish Journal of Veterinary & Animal Sciences
Thirty-four Streptococcus thermophilus strains were isolated from yogurt samples obtained from various villages in different regions of Turkey. These strains were examined for their antibiotic resistance patterns and plasmid carriage. The susceptibility of the isolates to 8 antibiotics (vancomycin, tetracycline, chloramphenicol, erythromycin, cephalothin, ampicillin-sulbactam, gentamicin and penicillin G) was determined by the disk diffusion method. Most strains of S. thermophilus were resistant to gentamicin (79%) and penicillin G (64%) and susceptible to chloramphenicol (94%) and tetracycline (88%). In plasmid DNA analyses, it was determined that 7 strains did not contain any plasmid DNA, while other strains included plasmid DNA ranging …
Antibiotic Resistance Of Salmonella Enteritidis Of Human And Chicken Origin, Gülşen Goncagül, Elçi̇n Günaydin, K. Tayfun Çarli
Antibiotic Resistance Of Salmonella Enteritidis Of Human And Chicken Origin, Gülşen Goncagül, Elçi̇n Günaydin, K. Tayfun Çarli
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to examine the relationship between antibiotic resistance patterns among Salmonella enterica subsp. enterica Serovar Enteritidis isolates (Salmonella Enteritidis) of human and poultry origin. Antibiotic resistance of 97 Salmonella Enteritidis isolates from 25 chicken meat, 25 chicken intestine and 47 human fecal samples was examined using the National Committee for Clinical Laboratory Standards (NCCLS, 1997) disk diffusion method. Resistance patterns of the isolates were as follows: Of the 25 chicken meat isolates 4 were resistant to some antibiotics. Two isolates (CM15 and CM22) were resistant to ampicillin (AMP), cefuroxime sodium (CXM), ceftriaxone (CRO), trimethoprim-sulphamethazole (SXT) …