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Medicine and Health Sciences

Turkish Journal of Veterinary & Animal Sciences

2017

Immunohistochemistry

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Full-Text Articles in Life Sciences

Immunohistochemical Expression Of Surfactant Apoproteins In Pneumonic Ovine Lungs, Enver Beytut Jan 2017

Immunohistochemical Expression Of Surfactant Apoproteins In Pneumonic Ovine Lungs, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

The present study was undertaken to investigate the immunohistochemical expression of pulmonary surfactant proteins (SP-A, SP-B, proSP-C) associated with proliferating cell nuclear antigen (PCNA), thyroid transcription factor-I (TTF-I), and lymphocytic phenotypes (CD3+ T and CD79αcy+ B cells) in ovine lungs with exudative and verminous pneumonia. Pneumonic lungs (n = 50) were classified as presenting fibrinous bronchopneumonia (n = 16), abscessing necrotic bronchopneumonia (n = 10), verminous pneumonia (n = 15), and interstitial pneumonia (n = 9). The lungs with fibrinous bronchopneumonia displayed consolidation of lobes with adhesion to surrounding tissues. The lungs with verminous pneumonia were characterized by lungworm sections …


Extraskeletal Chondroblastic Osteosarcoma Of The Subcutaneous Tissue In A Maltese Dog, Byungjoon Seung, Junghyung Ju, Seunghee Cho, Soohyeon Kim, Hwan Choi, Junghyang Sur Jan 2017

Extraskeletal Chondroblastic Osteosarcoma Of The Subcutaneous Tissue In A Maltese Dog, Byungjoon Seung, Junghyung Ju, Seunghee Cho, Soohyeon Kim, Hwan Choi, Junghyang Sur

Turkish Journal of Veterinary & Animal Sciences

A 10-year-old spayed female Maltese dog presented to a local animal hospital with a subcutaneous mass (4 x 3 x 3 cm) in the right shoulder region. The mass was well circumscribed, with soft tissue opacity and variable levels of mineralization, but with no bony involvement in radiography. The mass was surgically removed. Upon histological examination, the mass consisted of malignant mesenchymal cells that had produced a chondroid matrix and osteoid. Round to polygonal neoplastic cells with large nuclei showed moderate anisokaryosis and variable numbers of mitotic figures. The tumor cells were positive for vimentin and osteocalcin and negative for …


Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut Jan 2017

Pathological And Immunohistochemical Evaluation Of Skin And Teat Papillomas In Cattle, Enver Beytut

Turkish Journal of Veterinary & Animal Sciences

Bovine skin and teat papillomas were examined for immunohistochemical expression of bovine papillomavirus (BPV), proliferating antigens (PCNA, Ki-67), CD3+ T lymphocytes, and some of the type III intermediate filaments. Papillomas showed epidermal hyperplasia, koilocytes, and hyperkeratosis with prominent dermal connective tissue. Immunohistochemistry found the presence of BPV antigens in the nuclei of granulosa cells. PCNA and Ki-67 revealed positive nuclear staining in the dermis and epidermis. Perivascular cuffing in the dermis predominantly consisted of CD3+ T cells. Immunoreaction to vimentin was strong in the connective tissue of the dermis. $\alpha$SMA positivity was mainly in the media of vessels. Smooth muscle …