Life Sciences Commons^™

Snac_Oshc: Exploring A Multifaceted Approach To Develop Outside Of School Hours Care As A Health Promoting Setting, Karen Forde

Theses : Honours

Background: Outside of school hours care (OSHC) services are underutilised as health promoting settings, yet are well positioned to influence health behaviours. OSHC Professionals are ideally placed to become positive influencers in this setting, although may require training to confidently perform this role.

Aim: This research trialed a multifaceted intervention strategy to increase OSHC Professional’s confidence and competencies, to support a health promoting OSHC environment with a nutrition and PA activity focus.

Design and Methods: This exploratory study adopted a mixed methods approach. The three-pronged, multifaceted intervention included: a workshop, a closed Facebook group, and a website. 19 OSHC Professionals, …

Investigating The Role Of Dachshund Homolog 1 (Dach1) And Mir-200b In Group 4 Medulloblastoma Pathogenesis, Courtney George

Theses : Honours

Medulloblastoma is the most common malignant childhood brain tumour, and the most significant cause of childhood cancer-related mortality. Recently four core molecular medulloblastoma sub-groups have been identified, with distinct pathogenesis and responses to therapies. Current therapies, do not account for this molecular variation, and many patients may receive inappropriate treatment. To address this, targeted therapies for each molecular sub-group would be ideal. Unfortunately, for the more aggressive Group 3 and Group 4 subgroups, the underlying mechanisms of pathogenesis remain poorly understood. The current challenge is to identify the key tumour suppressors or oncogenes involved in Group 3 and Group 4 …

Self-Efficacy And Nutrition Knowledge Of Parents In Western Australia, Karen Lombardi

Theses : Honours

Background: Poor dietary choices and increasingly sedentary lifestyles mean childhood obesity is an increasing problem. Parents have the capacity to significantly affect the food choice of children; therefore by improving their self-efficacy, their nutrition knowledge, attitudes and behaviour, and by providing access to sound nutritional information, the health of children may be improved.

Purpose: This research sought to gather preliminary information required to inform the development of nutrition education materials for parents, which will parallel those currently under development for teaching children in schools as part of the Australian curriculum. Research has demonstrated that while educating children about healthy eating …

The Effect Of Antioxidant Supplementation On Human Sperm Cryopreservation, Katrina E. Taylor

Theses : Honours

Semen cryopreservation has an important role in assisted reproductive technology however, the cooling, freezing and thawing processes often result in a significant loss of sperm motility, viability and nuclear integrity. The destructive effects of cryopreservation are significantly exacerbated in samples exhibiting low sperm number and poor morphological characteristics. Recent research into infertility has focused on the correlation between excessive oxidation and subfertility, in particular radical induced lipid peroxidation within the phospholipid bilayer of the spermatozoon plasma membrane and the promotion of cellular damage as a result of antioxidant insufficiency. The aim of this study was to evaluate the effect of …

Changes In Muscle Function And Motorneuron Excitability Of The Triceps Surae Following A Bout Of Fatiguing Eccentric Exercise, Mikala Pougnault

Theses : Honours

A reduction in capacity of the neuromuscular system associated with exercise can occur from a wide range of physiological and psychological factors. Many researchers have investigated neural activation during exercise, or the effects of muscle damage associated with eccentric exercise, but few have studied the prolonged effects of a bout of eccentric exercise on strength and motorneuron excitability. Eleven male and female subjects (aged 20-43 years) were tested to determine the effects of a fatiguing bout of eccentric exercise upon maximal isometric plantarflexion strength, motorneuron excitability, and neural activation of the soleus (SOL) and medial gastrocnemius (MG). The exercise consisted …

The Effect Of Moderate +Gz On Cervical Muscle Strength Of Raaf Trainee Pilots Flying Pc-9 Aircraft, Elissa Jane Burton

Theses : Honours

External stimulus/loading initiates adaptations within skeletal muscle. Whilst performing flying manoeuvres under +Gz it has been previously found that the cervical area has the highest loading. The purpose of this study was to examine cervical muscle response to moderate +Gz force (+4-6Gz) loading generated during RAAF pilot training. Cervical muscle strength was monitored in nine RAAF pilots completing an eight-month flight training course and ten controls matched for gender, age, height and weight. Cervical muscle strength and range of movement were measured at baseline and at eight months using the Multi-Cervical Rehabilitation Unit (Hanoun, Canada). Also measured, using EMG, was …

Sense Of Effort Associated With Exercise In The Chronic Fatigue Syndrome, Karen E. Wallman

Theses : Honours

Investigations into the mediators of effort sensation have indicated that central mechanisms related to corollary discharges may be responsible for an increased sense of effort during fatiguing isometric exercise. The role for central mediators for sense of effort have been objectively demonstrated through use of contralateral limb matching tasks. Subjects diagnosed with chronic fatigue syndrome (CFS) often report prevalent fatigue associated with a greater sense of effort when involved in exercise. This study employed a fatiguing contralateral limb-matching task in order to determine if CFS subjects (n == 6) experienced an altered sense of effort associated with the task when …

The Role Of Fas And Fas Ligand In Apoptosis During Regression Of The Corpus Luteum, Sharon Roughton

Theses : Honours

Apoptosis, a form of physiological cell death, has been found to occur during regression of the corpus luteum (Juengel etal, 1993; Dharmarajan etal, 1994). The pathways involved in this process, however, have yet to be specified. One possible mediator of corpus luteum regression is the Fas (or AP0-1 or CD95) receptor, a transmembrane protein which induces apoptosis in the cell when ligated. In order to further confirm this hypolhesis, the present study establishes and quantitates the presence and regulation of Fas receptor and Fas ligand (Fasl) in the rat corpus luteum during pregnancy and post-partum. The animals used were sexually …

Localisation Of The Gene For A Novel Form Of Charcot-Marie-Tooth Disease In An Isolated Population, Kaite Honeyman

Theses : Honours

Localising the gene for a previously undescribed autosomal recessive form of CMT involved the use of a relatively new approach to rapid genome screening based on the identification of segments which are inherited identical by descent (IBD) from common founding ancestors. It is most feasible for populations which have been founded relatively recently (say less than 25 generations) and which have remained relatively isolated either geographically or culturally. The method is not suitable for highly inbred populations, that is with first and second cousin matings, as many segments will be inherited by chance. It appears to be a suitable screening …

Is There A Difference In Cortical Representation Between Dominant And Non-Dominant Arm Muscles Of Elite Badminton Players?, Dylan J. Edwards

Theses : Honours

Training for sport involves the development of skill and coordination. The physiological changes associated with skill acquisition are complex and at present poorly understood. One of the areas in the central nervous system thought to be involved with skill acquisition is the cerebral motor cortex where localised areas are responsible for controlling specific muscle groups. Learning or improving a motor skill may require reorganisation of the cortical areas controlling relevant muscles to accommodate the new skill. To test this idea we studied a group of elite badminton players that were highly skilled in their dominant playing arm. Transcranial magnetic stimulation …

Effect Of Exercise Induced Muscle Soreness On The Motor Control Properties Of The Biceps Brachii, Alan J. Pearce

Theses : Honours

The objective of this study was to note the time course changes for up to 28 days on the motor control properties of biceps brachii muscle following a bout of eccentric exercise. Eight subjects (5 male, 25-40 years of age) performed 35 maximal voluntary eccentric contractions with the non-preferred arm of the elbow flexors through 130° of extension of 90°s^-1. Voluntary electromyographic (EMG) activity and motor evoked potentials (MEPs) elicited by transcranial magnetic stimulation (TMS) were recorded via surface electrodes placed over the belly of the biceps brachii muscle. Maximal isometric strength was measured at 90° elbow flexion. …

Localisation And Detection Of A Polymorphism In The Human Skeletal Beta-Tropomyosin Gene (Tpm2), Clive C.J. Hunt

Theses : Honours

Tropomyosin is one of the components of the thin filaments of muscle, binding to actin, and, together with troponin, regulating contraction in a calcium-dependent manner (Cho et al.,1990). There are at least four distinct tropomyosin genes in vertebrates and each may encode at least six different isoforms of tropomyosin by alternate splicing (Novy et al, 1993; MacLeod et al., 1988). The alpha-tropomyosin gene TPM1 has recently been localised to 15q22 (Eyre et al, 1994) and has been shown to be mutated in some cases of familial hypertrophic cardiomyopathy (Thierfelder et al., 1994). The alpha-tropomyosin gene TPM3 has been recently localised …

Detection Of Point Mutations In The Dystrophin Gene, John Pedretti

Theses : Honours

The dystrophin gene has been localised to Xp 21.1. Mutations of this gene can lead to the clinical manifestations of Duchenne and Becker muscular dystrophies (DMD/BMD). In the majority of DMD and BMD patients the disease-causing mutation is a deletion detectable by southern analysis or multiplex PCR, however in 30% of patients no deletion is observed using these conventional tests. Using PCR amplification of cDNA it was possible to detect a deletion in the product of the dystrophin gene of one such individual affected with BMD. It was then necessary to characterise the mutation in order to determine whether this …