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The Fecal Shedding Of Listeria Monocytogenes By Conventional And "Germ Free" Mice, Margaret Joyce Ramsay
The Fecal Shedding Of Listeria Monocytogenes By Conventional And "Germ Free" Mice, Margaret Joyce Ramsay
Loma Linda University Electronic Theses, Dissertations & Projects
A series of experiments involving Listeria monocytogenes was undertaken to study first the fecal shedding of Listeria by conventional mice challenged intraperitoneally with Listeria and second the fecal shedding and ability to survive of germ free mice with similar challenge. In addition, mites infesting Listeria infected mice were cultured for their possible contamination by Listeria.
Conventional mice were challenged intraperitoneally with a single near LD50 dose of Listeria. Fecal Listeria were detected by collecting specimens from each mouse daily for the first 4 days and every second day thereafter. The feces was held at 7°C for 14 …
Biochemical Properties Of Salmonella Potsdam Bacteriophage P4/2, Clyde L. Roggenkamp
Biochemical Properties Of Salmonella Potsdam Bacteriophage P4/2, Clyde L. Roggenkamp
Loma Linda University Electronic Theses, Dissertations & Projects
Over 90% recovery of phage P4/2 was realized with differential centrifugation concentration and purification, whereas only about 16% was recovered from the aqueous two-phase polymer - Genetron - ECTEOLA-cellulose column system. Purity of preparations was greatest with the density gradient centrifugation (A260 = 2.66 and A280 = 1.80 per 1012 plaque-forming units). With the chemical system of concentration and purification followed by an ECTEOLA anion exchange run, the A260 was 6.6 and the A280 was 4.3.
Three tests were employed to determine the configuration of P4/2 DNA: (1) a formalin denaturation test, (2) a thermal …
A Growth Study On Phenylketonurics, Emma Johnson Aitken
A Growth Study On Phenylketonurics, Emma Johnson Aitken
Loma Linda University Electronic Theses, Dissertations & Projects
Data from forty-eight medical records of phenylketonuria children under treatment were investigated to determine if growth, as measured by stature, was normal; and to determine if the state of control or the age at beginning of treatment had an effect on growth. Mean intakes of phenylalanine, protein, and calories were obtained on the nutritional data only if the simultaneous serum phenylalanine was equal to or less than 6 milligrams per cent. These means were grouped according to the age of the children into 7 groups (0
The height at beginning of treatment and the last measured height was compared with …
A Comparative Study Of The 5- And 3-Meal Plans In Six Convalescent Hospitals In San Bernardino County, Donna J. Meek
A Comparative Study Of The 5- And 3-Meal Plans In Six Convalescent Hospitals In San Bernardino County, Donna J. Meek
Loma Linda University Electronic Theses, Dissertations & Projects
A study comparing the 3- and 5-meal plans of food service, as used in long-stay geriatric units in San Bernardino County, have been compared. Three homes utilizing the 5-meal plan and three utilizing the 3-meal plan, to serve as controls, were chosen. A random sample of ten patients from each home was observed for three days to find the daily average nutrients in the foods served, eaten, and wasted. Also, interviews to compare factual information and attitudes were utilized for sample patients and personnel. The nutritional data was analyzed on the computer.
The results indicated that those on the 5-meal …
An Organ Culture Technique For Maintaining The Pulp Tissue Of Intact Human Teeth, John P. Devincenzo
An Organ Culture Technique For Maintaining The Pulp Tissue Of Intact Human Teeth, John P. Devincenzo
Loma Linda University Electronic Theses, Dissertations & Projects
The size of explants in organ culture is limited by their ability to feed, respire, and excrete by diffusion. The perfusion procedure described in this report is not limited to a particular explant size. The test tissue was the adult human tooth.
In order to maintain the pulp tissue of the intact human tooth in organ culture, special precautions were taken to prevent internal necrosis and to allow diffusion of nutrients and waste products. Using aseptic technique, a standard tissue culture medium plus appropriate antibiotics were delivered to a glass manifold housed in an incubator with an atmosphere at 100% …