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Subnuclear Distribution Of The Vitamin D Receptor, Joseph Bidwell, Andre Van Wijnen, Edward Fey, Harold Merriman, Sheldon Penman, Janet Stein, Gary Stein, Jane Lian
Subnuclear Distribution Of The Vitamin D Receptor, Joseph Bidwell, Andre Van Wijnen, Edward Fey, Harold Merriman, Sheldon Penman, Janet Stein, Gary Stein, Jane Lian
Harold L. Merriman
The subnuclear distribution of the vitamin D receptor was investigated to begin addressing the contribution of nuclear architecture to vitamin D-responsive control of gene expression in ROS 17/2.8 rat osteosarcoma cells. The nuclear matrix is an anastomosing network of filaments that is functionally associated with DNA replication, transcription, and RNA processing. The representation of vitamin D receptor in the nuclear matrix and nonmatrix nuclear fractions was determined by the combined application of 1) sequence-specific interactions with the vitamin D receptor binding element of the rat bone-specific osteocalcin gene promoter and 2) Western blot analysis. Both methods confirmed the presence of …