Life Sciences Commons^™

The Effects Of A Wild Blueberry Diet On Hepatic And Aortic Morphology In The Obese Zucker Rat, A Model Of The Metabolic Syndrome, Thomas Merrow

Electronic Theses and Dissertations

The goal of this study is to investigate the effect of a wild blueberry diet on pathology of the metabolic syndrome (MetS) by examining the morphological and biochemical properties of the liver and aortic tissue in the obese Zucker rat (OZR), a valid model of the MetS.

At 8-weeks of age, 16 Obese Zucker ras (OZR) and 16 lean Zucker rats (LZR) littermates were placed on either an 8% w/w wild blueberry (WB)-enriched isocaloric diet or an isocaloric control (C) diet for a duration of 8-weeks. At 16-weeks of age, the tissues of interest were harvested for the study. The …

Dynamic Host-Pathogen Interactions Result In Fungal Epitope Unmasking, Alex Hopke

Electronic Theses and Dissertations

Molecular camouflage is used by a diverse set of pathogens to disguise their identity and avoid recognition by protective host receptors. The opportunistic fungal pathogen Candida albicans is a good example, as it masks the inflammatory component β-glucan in its cell wall to evade detection by the immune receptor Dectin-1. Interestingly, it has been seen that β-glucan becomes unmasked during infection in vivo, though the underlying mechanisms remained unclear. Exposure levels of this epitope may be important, as Dectin-1 mediates protection from some strains of C. albicans and alterations in the organization and composition of the Candida cell wall …

Impact Of Luminance And Spatial Parameters On The Generation Of The Human Pattern Electroretinogram., Kate A. Godwin

Electronic Theses and Dissertations

The current work assessed some of the key hypotheses behind the generation of the pattern electroretinogram (PERG) response. The first of these hypotheses states that the PERG response is the result of linear cancellation of simultaneous increment and decrement retinal responses, as generated by the retinal ON- and OFF-pathways. Experiment 1 evaluated the possibility of simulating the PERG by summing the ERG responses elicited by increment and decrement flashes, and found that it was indeed possible to simulate the PERG from these responses. However, only the steady-state PERG could be modeled consistently. The second hypothesis evaluated a theory that the …

The Role Of O-Glcnacase During Heart Failure., Sujith Dassanayaka

Electronic Theses and Dissertations

Global augmentation of protein O-GlcNAcylation occurs in response to a myriad of stressors and confers a survival advantage at the cellular level. This protective phenomenon has been demonstrated to mediate cardioprotection through various in vitro and in vivo studies during ischemia-reperfusion, myocardial infarction, and oxidative stress; however, relatively little is known of the regulation of protein O-GlcNAcylation. Protein O-GlcNAcylation is regulated by two antagonistic enzymes, namely, O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA). Ablation of cardiomyocyte OGT, the enzyme that catalyzes the addition of O-GlcNAc to proteins, exacerbates cardiac dysfunction during infarct-induced heart failure (HF). However, little is known of the …

Air Pollution, Pulmonary Oxidative Stress, And The Endothelin System In The Development Of Cardiovascular Injury., Jordan B. Finch

Electronic Theses and Dissertations

The goal of this project was to examine the role of endothelin-1 (ET-1) as a mediator in the pathway between air pollution exposure and the development of vascular injury. A human cohort and male mice (C57BL/6 and ecSOD-Tg) were used to evaluate changes in the ET-1 system in response to exposures of fine particulate matter (PM2.5). Human ET-1 levels were significantly associated with environmental factors and markers of vascular change, but were decreased with increased PM2.5. No association was seen between ET-1 and endothelial progenitor cells (EPCs) except for EPC-4, possibly indicating a regulatory relationship with this specific population. In …

The Combined Effects Of Leptin And Coenzyme Q10 In Ameliorating Obesity- Induced Infertility In Female Rats, Adekunle Adedeji

Electronic Theses and Dissertations

Infertility is one of the major problems of obesity. Studies have shown that administration of leptin reversed obesity-induced infertility in rats and mice. Coenzyme Q₁₀ (CoQ₁₀) is an antioxidant and also supplies the energy needed for ovulation and embryo development. We hypothesized that leptin when combined with CoQ₁₀ could greatly improve obesity-induced infertility. The results showed a significant decrease in food intake, body weight, and the regular estrous cycle was restored after treatment with leptin+CoQ₁₀. There was a significant increase (p10 significantly (p10 can improve fertility in obese infertile female rats. This study could …

Endothelial Hspa12b Is A Novel Protein For The Preservation Of Cardiovascular Function In Polymicrobial Sepsis Via Exosome Mir-126, Xia Zhang

Electronic Theses and Dissertations

Sepsis is the most frequent cause of mortality in most intensive care units. Cardiovascular dysfunction is a major complication associated with sepsis, with high mortality rates up to 70%. Currently, there is no effective treatment approach for sepsis.

The integrity of the endothelium is fundamental for the homeostasis of the cardiovascular system. Sepsis induces endothelial cell injury which is the key factor for multiple organ failure. The increased expression of adhesion molecules and chemokines in endothelial cell promotes leukocytes infiltration into the tissue. The loss of tight junction proteins and increased permeability of the endothelial cells will provoke tissue hypoxia …

Eating For Environmental And Personal Health: Green Eating, Health Behavior And Environmental Perceptions At The University Of Maine, Moira E. Burke

Electronic Theses and Dissertations

The purpose of this study was to determine if green eating (GE), defined as environmentally conscious eating, in college students had an impact on BMI, health behavior, fruit and vegetable intake, physical activity, and perceptions of the campus environment. This was a cross-sectional study of undergraduate and graduate students (n=190) at the University of Maine. The GE Survey was used to determine GE stage of change; participants were classified as either pre-action (precontemplation, contemplation, preparation) (n=113) or post-action (action, maintenance) (n=77). The GE Survey was also used to assess GE behavior, decisional balance, and self-efficacy. A de novo health behavior …

Comparison Of Two Body Weight Screenings Of Self-Conscious Emotions And Coping, Rachel N. Nanez Ms.

Electronic Theses and Dissertations

Novel technologies are currently being utilized in fitness and clinical settings. However, there is not much research to examine the emotional responses to such screenings.

PURPOSE: To examine the group differences between the FIT3D and DXA protocol on emotional responses and the differences between weight classifications.

METHODS: The Body and Appearance-related Self-conscious Emotions Scale and WEIGHTCOPE were used to assess self-conscious emotions and intention to utilize 10 different weight-related coping strategies following the scans. Motivation was measured by a 10-point Likert scale measuring motivation pre and post scans.

RESULTS: There were no differences in motivation, affective response, or intention to …

Stop The Pricks: A Validation Study Of A Noninvasive Lactate Threshold Device, Rebecca M. Mcmorries

Electronic Theses and Dissertations

ABSTRACT

Purpose: The aim of this study is to assess the validity of a noninvasive lactate threshold device (NID) to determine lactate threshold heart rate (LTHR) and aerobic threshold heart rate (AeTHR).

Methods: Twenty-one recreational athletes completed a personalized graded exercise test on a treadmill. All participants wore the noninvasive device and blood lactate samples were taken at the end of 3-minute stages. Lactate threshold heart rate and aerobic threshold heart rate were then calculated using four traditional methods and compared against the same heart rate values calculated by the device.

Results: No significant differences were found in lactate threshold …

A Competitive Assessment Of Commercial Elderberry (Sambucus Sp.) Products And The Evaluation Of Copigmentation Within Elderberry Tinctures, Joseph A. Galetti Phd

Electronic Theses and Dissertations

Elderberry (Sambucus sp.) fruit is a healthful food with a variety of reported curative properties and is among the richest sources of anthocyanic pigmentation, which are the primary factors for its commercial use. Although a variety of value-added elderberry products are available to consumers, it is questionable as to which product form contains the highest nutrient levels and color stability characteristics, and represents the best value to consumers. It was the objectives of this research to evaluate a variety of commercial elderberry products, and to develop a value-added elderberry product with enhanced nutrient and color stability.

The first part …

Investigation Of Novel Functions For Dna Damage Response And Repair Proteins In Escherichia Coli And Humans, Benjamin A. Hilton

Electronic Theses and Dissertations

Endogenous and exogenous agents that can damage DNA are a constant threat to genome stability in all living cells. In response, cells have evolved an array of mechanisms to repair DNA damage or to eliminate the cells damaged beyond repair. One of these mechanisms is nucleotide excision repair (NER) which is the major repair pathway responsible for removing a wide variety of bulky DNA lesions. Deficiency, or mutation, in one or several of the NER repair proteins is responsible for many diseases, including cancer. Prokaryotic NER involves only three proteins to recognize and incise a damaged site, while eukaryotic NER …

Role Of Microrna-21 In Atherogenesis., Rihab Hamed-Berair

Electronic Theses and Dissertations

MicroRNA-21 (miR-21) is an evolutionarily conserved microRNA, abundant in most cardiovascular tissues. It has been implicated in the pathogenesis of several cardiovascular diseases including restenosis, myocardial infarction, and heart failure. However, little is known about the contribution of miR-21 in atherosclerosis. My data show that expression of miR-21 is increased by >1.5-fold in murine atherosclerotic lesions and by 1.5-2.0-fold in the macrophages of Western diet (WD)-fed LDLR-KO mice (for 12-20 weeks). In vitro, LDL, oxidized LDL, acetylated LDL and LPS induced miR-21 by 2-4-fold and down-regulated its target protein, PDCD4, in bone marrow-derived macrophages. Basally, macrophages isolated from miR-21-KO …

Sa-4-1bbl As A Modulator Of Innate, Adaptive, And Regulatory Immunity : Implications For Cancer Prevention And Treatment., Hampartsoum Barsoumian

Electronic Theses and Dissertations

SA-4-1BBL is a recombinant costimulatory molecule that is active in its soluble form and has pleiotropic effects on the functions of innate, adaptive, and regulatory immune cells. We have previously shown that SA-4-1BBL makes CD4+ T conventional cells (Tconvs) resistant to suppression by CD4+CD25+Foxp3+ T regulatory cells (Tregs). The mechanistic basis of this observation is the subject of this study. We demonstrate that Tconvs, but not Tregs, are the direct targets of SA-4-1BBL-mediated evasion of Treg suppression without contribution from antigen presenting cells (APCs). SA-4-1BBL engagement with its receptor, 4-1BB, on Tconvs resulted in the production of high levels of …

Microrna-186 And Metastatic Prostate Cancer., Dominique Zilpha Jones

Electronic Theses and Dissertations

MicroRNA (miR) dysregulation alters cancer-associated gene expression, which contributes to cancer pathogenesis. For example, miR-186 over expression lead to enhanced proliferation and migration in pancreatic cancer cell models. However, the role of miR-186 in prostate cancer (PCa) remains controversial. Previously, miR-186-5p was up-regulated in PCa patient serum (stage III/IV) compared to controls. Furthermore, miR-186-5p was up-regulated in metastatic PCa (PC-3, MDA PCa 2b, LNCaP) relative to normal prostate epithelial cells (RWPE1). We hypothesized miR-186 inhibition will reduce aggressive PCa using metastatic cell models. To test this, we evaluated whether miR-186-5p inhibition would reduce aggressive PCa behavior and overexpression induce malignant …

Mathematical Modeling Of Heart Rate Deflection Point In Relation To Respiratory Compensation And Treadmill Running Performance, Kayla Baker

Electronic Theses and Dissertations

Heart rate deflection point (HRDP), identified as the second breakpoint in the "intensity / heart rate" relationship, is indicative of the anaerobic threshold (AT). This point can be determined via bi-segmental linear regression (2SEG) or through use of the maximal distance model (D-max); however, the relationship between these methods has yet to be investigated 2. Purpose: To compare the use of 2SEG and D-max methods to determine HRDP and to examine the relationship between these values with a metabolic threshold, respiratory compensation point (RCP), as well as running performance [5,000 meter treadmill time trial (5Ktime)]. Methods: Nineteen recreationally active men …

The Reproducibility And Validity Of Using The Dmax Method To Predict Physical Working Capacity At Fatigue Threshold, Joshua Riffe

Electronic Theses and Dissertations

Although the original (ORG) physical working capacity at fatigue threshold (PWCFT) assessment has demonstrated strong reliability and sensitivity to training and/or nutritional interventions, limitations may exist regarding the method of PWCFT estimation. However, a relatively new mathematical method, called Dmax, has been shown to be objective and reliable when estimating metabolic and neuromuscular fatigue thresholds. To date, however, no study has examined whether the Dmax method for estimating the work rate at PWCFT is similar to the ORG method. PURPOSE: The purpose of this study is to evaluate the reliability and validity of the Dmax-EMG method for estimating and tracking …

Evaluation Of Echogenicity Within And Between Ultrasonographic Images Of The Vastus Lateralis, Alyssa Varanoske

Electronic Theses and Dissertations

PURPOSE: The purpose of this study was to examine the echo intensity (EI) values of different ultrasound (US) images of the vastus lateralis (VL) using panoramic imaging in the transverse plane (PTI) and still imaging in the longitudinal plane (SLI). Secondary purposes of this study were to examine VL homogeneity and to determine relationships between subcutaneous adipose tissue thickness (SubQ) adjacent to the muscle and EI. METHODS: Twenty-four recreationally trained collegiate males (20.2 ± 1.6 years; height: 178.1 ± 6.6 cm; weight: 82.2 ± 13.4 kg) participated in this investigation. EI, cross-sectional area (CSA), muscle thickness (MT), and SubQ of …

Longitudinal Examination Of Perceived Stress And Depression Symptomology In Division I Student-Athletes, Bobby Daigle

Electronic Theses and Dissertations

CONTEXT: Concussions are all too prevalent in amongst athletes. Concussions make up almost 5 percent of all collegiate athletic injuries. Concussions have been linked with many long lasting effects including depression and increased stress or anxiety.

OBJECTIVE: To longitudinally examine post-concussion depression and stress levels in National Collegiate Athletic Association Division 1 athletes.

DESIGN: Descriptive longitudinal study.

SETTING: National Collegiate Athletic Association Division I collegiate athletics.

PARTICIPANTS: Concussed and uninjured Division I collegiate athletes, ages 18-22, competing in football, women’s soccer, baseball, softball, and women’s track.

INTERVENTION(S): Participants completed the CES-D at baseline, 1 week, 1 month, and 3 months …

Effects Of A 10-Week Introductory Judo Course On Postural Control During Reactionary Bilateral Gripping Task With Varied Stances And Lower Body Power Performance, Tyler Muddle

Electronic Theses and Dissertations

PURPOSE: The purpose of the present study is twofold: 1.) Examine the effects of 10-weeks of an introductory judo course on postural control during maximal bilateral isometric handgrip testing using different stance conditions and lower body power performance, and 2.) To analyze the relationship between maximal bilateral handgrip exertions on postural control during varied stance conditions. METHODS: Twenty recreationally active men and women divided into two an experimental group, (JDO) (n = 10; 21.70 ± 3.83 y; 169.91 ± 6.01 cm; 73.89 ± 12.10 kg; 19.01 ± 8.06% BF), and a control group, (CON) (n = 10; 21.50 ± 2.84 …

The Correlation Between Strength Levels Measured Through Dynamic Strength Exercises And The Incidence And Severity Of Injury Among Collegiate Athletes, Alex Jardine

Electronic Theses and Dissertations

Injury to athletes in sport occurs as a result of extrinsic and intrinsic risk factors. Mounting evidence points toward decreased strength as a predictor of injury in athletes. By comparing strength levels in functional movement patterns to injury, the strength and conditioning professional will be able to design effective training programs to reduce the incidence of injury in sport better. The purpose of this study was to determine the correlation between strength measured through dynamic strength exercises (power clean, the squat, and the bench press) among collegiate athletes and the incidence and severity of injury. We hypothesized that greater incidence …

Icook 4-H: 0 To 24-Month Accelerometer-Derived Physical Activity And Sedentary Time In Youth, Emily Hofer

Electronic Theses and Dissertations

To assess accelerometer-derived physical activity and sedentary time from 0 to 24- months in youth in the iCook 4-H program. The iCook 4-H Program was a 5-state, randomized, control-treatment, family-based childhood obesity prevention intervention promoting cooking, eating and playing together. Youth, 9-10 years old, and their main adult meal preparer, participated in the 12-week program followed by monthly newsletters and bi-yearly booster sessions until 24-months. Physical activity and sedentary time were determined for youth who wore an Actigraph GT3X+ accelerometer for 7 days at 0, 4, 12, and 24-months and met defined accelerometer compliance standards. Mean daily minutes of sedentary …

Influencers And Barriers To Consumption Of Healthful Diets In Rural Youth In Context Of The Bioecological Model, Megan Bren

Electronic Theses and Dissertations

The purpose of this study is to determine influencers and barriers to healthful dietary intake in rural youth in context of the bioecological model. Four focus groups as part of Ignite, a tri-state, five-year, community based partipatory research (CBPR) intervention were conducted with 6th to 8th grade adolescents from an economically disadvantaged community of rural South Dakota. Results were categorized into the context of the bioecological model. Within the model, the process, person, context and time (PPCT) design with the three types of personal characteristics were used to describe the framework in which the systems are embedded. Focus group content …

Influence Of Cardiovascular Risk Factors On Remote Ischemic Preconditioning, Tiffany Trachte

Electronic Theses and Dissertations

A powerful therapy against microvascular endothelial ischemia-reperfusion injury is remote ischemic preconditioning (rIPC), which triggers tissue protection by exposing a limb to small cycles of vascular occlusion. Animal models indicate that CVD risk factors reduce the protective benefits of ischemic conditioning. However, there are no human studies investigating how a burden of risk factors interferes with rIPC to prevent endothelial injury. The purpose of the present study was to determine the influence of risk factor burden on the capacity of rIPC to prevent endothelial reperfusion injury in humans. Twenty-two (age: 45±14 yr., BMI: 31±8 kg/m2) sedentary adults (12 lower burden: …

Differences In Running Mechanics Between Overweight/Obese And Healthy Weight Children, Kristen Roles

Electronic Theses and Dissertations

Background/Purpose: Physical activity is commonly prescribed to reduce childhood obesity. However, due to differences in mechanics during low-impact activities, such as walking, obese children may be more prone to negative physical complications during high-impact activities, such as running. Therefore, this study analyzed the mechanical differences in running mechanics between healthy weight (HW) and overweight/obese (OV/OB) children. We hypothesized that when compared to HW children, OV/OB children would display higher vertical loading, greater joint moments and greater joint angular impulses during running. We also expect decreased sagittal plane range of motion and increased frontal plane range of motion of the hip, …

Development And Characterization Of A Recombinant Orf Virus Vector Expressing The Spike Protein Of Porcine Epidemic Diarrhea Virus, Kyle Hain

Electronic Theses and Dissertations

Orf virus (ORFV), the type member of the genus Parapoxvirus of the family Poxviridae, causes orf or cutaneous pustular dermatitis in sheep and goats. ORFV is a ubiquitous virus capable of re-infecting its hosts multiple times over time. ORFV causes a non-systemic, self-limiting disease which is usually restricted to the skin surrounding the virus entry sites. ORFV has evolved several immunomodulatory proteins (IMPs) that evade and/or modulate host immune responses to infection and contribute to virus virulence and disease pathogenesis. Given biological properties and unique immunomodula tor y properties, ORFV has gained significant attention in recent years for its …

Role Of Microglial Α7 Nicotinic Acetylcholine Receptor Positive Allosteric Modulator In Neuroinflammatory Pain Models In Mice, Muzaffar Abbas

Electronic Theses and Dissertations

Neuroinflammatory pain affects about 1.5% of the United States population. Around 20-40% patients having neurological disorders are affected with neuroinflammatory pain. As a part of the limbic system, hippocampus is known to play a critical role in pain perception and processing, and is densely populated with microglial cells and α7 nicotinic acetylcholine receptors (nAChRs). Given the role of microglial α7 nAChRs in neuroinflammation, the α7 nAChRs have emerged as potential target for neuroinflammatory pain treatment. We hypothesized that microglial α7 nAChRs positive allosteric modulation in the hippocampus will decrease neuroinflammatory pain at behavioral, cellular, biochemical, and molecular level. The primary …