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Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Detergent Extraction Of A Presumptive Gating Component From The Voltage-Dependent Sodium Channel, W J. Culp, D T. Mckenzie
Detergent Extraction Of A Presumptive Gating Component From The Voltage-Dependent Sodium Channel, W J. Culp, D T. Mckenzie
Dartmouth Scholarship
A physiologically characterized radiolabeled neurotoxin complex obtained from venom of the scorpion Leiurus quinquestriatus has been used to identify detergent-solubilized presumptive sodium channel components in sucrose gradients. This toxin-binding component is found in extracts prepared from three sources of excitable membrane but appears to be absent from similar extracts prepared from nonexcitable membrane or from Torpedo californica membrane. Procedures that destroy the physiological activity of the Leiurus neurotoxin lead to a corresponding loss of toxin binding to the putative sodium channel component. The major component recognized by the Leiurus toxin sediments at 6.5 S. Scatchard analysis of quantitative binding experiments …
The Isolation Of Ecdysterone Inducible Genes By Hybridization Subtraction Chromatography, Michael P. Vitek, Susan G. Kreissman, Robert H. Gross
The Isolation Of Ecdysterone Inducible Genes By Hybridization Subtraction Chromatography, Michael P. Vitek, Susan G. Kreissman, Robert H. Gross
Dartmouth Scholarship
We have developed a procedure for selectively enriching a mRNA population for inducible sequences. Other than the induced mRNA species, the population of mRNA in control cells is approximately the same as the mRNA population in induced cells. Cytoplasmic mRNA from control cells is bound to oligo (dT)-cellulose and used as a template for reverse transcriptase, the oligo (dT) serving as a primer. After removing the template mRNAs, the cDNA-cellulose column is used to hybridize a population of mRNAs from induced cells. The non-hybridized poly A + RNAs are greatly enriched in the inducible sequences. We have used this technique …