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Preconditioning Induces Sustained Neuroprotection By Down Regulation Of Ampk, Louise D. Mccullough, Venugopal Reddy Venna, Jun Li, Sharon E. Benashski, Sami Tarabishy
Preconditioning Induces Sustained Neuroprotection By Down Regulation Of Ampk, Louise D. Mccullough, Venugopal Reddy Venna, Jun Li, Sharon E. Benashski, Sami Tarabishy
UCHC Articles - Research
Abstract Background and Purpose—Ischemic preconditioning (IPC) induces endogenous neuroprotection from a subsequent ischemic injury. IPC involves down-regulation of metabolic pathways. As Adenosine 5′-monophosphate-activated protein kinase (AMPK) is a critical sensor of energy balance and plays a major role in cellular metabolism, its role in IPC was investigated.
Methods—A brief 3 minute middle cerebral artery occlusion (MCAO) was employed to induce IPC in male mice 72 hours prior to 90 minute MCAO. Levels of AMPK and pAMPK, the active form of the kinase, were assessed after IPC. A pharmacological activator or inhibitor of AMPK was utilized to determine the …