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Full-Text Articles in Life Sciences
Utilization Of Transgenic Models In Evaluation Of Osteogenic Differentiation Of Embryonic Stem Cells, Dario Repic, Elena Torreggiani, Tiziana Franceschetti, Brya G. Matthews, Alexander C. Lichtler, Ivo Kalajzic
Utilization Of Transgenic Models In Evaluation Of Osteogenic Differentiation Of Embryonic Stem Cells, Dario Repic, Elena Torreggiani, Tiziana Franceschetti, Brya G. Matthews, Alexander C. Lichtler, Ivo Kalajzic
UCHC Articles - Research
Previous studies reported that embryonic stem cells (ESCs) can be induced to differentiate into cells showing a mature osteoblastic phenotype by culturing them under osteo-inductive conditions. It is probable that osteogenic differentiation requires that ESCs undergo differentiation through an intermediary step involving a mesenchymal lineage precursor. Based on our previous studies indicating that adult mesenchymal progenitor cells express αSMA, we have generated ESCs from transgenic mice in which an αSMA promoter directs the expression of red fluorescent protein (RFP) to mesenchymal progenitor cells. To track the transition of ESC-derived MSCs into mature osteoblasts, we have utilized a bone-specific fragment of …