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Full-Text Articles in Life Sciences
Use Of An Alpha-Smooth Muscle Actin (Smaa) Gfp Reporter To Identify An Osteoprogenitor Population, Zana Kalajzic, Haitao Li, Li-Ping Wang, Xi Jiang, Katie B. Lamothe, Douglas J. Adams, Hector L. Aguila, David W. Rowe, Ivo Kalajzic
Use Of An Alpha-Smooth Muscle Actin (Smaa) Gfp Reporter To Identify An Osteoprogenitor Population, Zana Kalajzic, Haitao Li, Li-Ping Wang, Xi Jiang, Katie B. Lamothe, Douglas J. Adams, Hector L. Aguila, David W. Rowe, Ivo Kalajzic
UCHC Articles - Research
Identification of a reliable marker of skeletal precursor cells within calcified and soft tissues remains a major challenge for the field. To address this, we used a transgenic model in which osteoblasts can be eliminated by pharmacological treatment. Following osteoblast ablation a dramatic increase in a population of α-smooth muscle actin (α-SMA) positive cells was observed. During early recovery phase from ablation we have detected cells with the simultaneous expression of SMAA and a preosteoblastic 3.6GFP marker, indicating the potential for transition of α-SMA+ cells towards osteoprogenitor lineage. Utilizing α-SMAGFP transgene, α-SMAGFP+ positive cells were detected in the …