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Full-Text Articles in Life Sciences
Teaching Central Dogma Of Biology Using Cell Free Environment, Lukas Nelson, Sudeshna Roy, Bobbi J. Bohnet, Tracy Chase, Brian Labelle, Nicholas Butzin, Madhav P. Nepal
Teaching Central Dogma Of Biology Using Cell Free Environment, Lukas Nelson, Sudeshna Roy, Bobbi J. Bohnet, Tracy Chase, Brian Labelle, Nicholas Butzin, Madhav P. Nepal
iLEARN Teaching Resources
In this lesson plan, students will learn and visualize how genetic information coded in DNA is transcribed into RNA which is further translated into protein. This entire process, DNA->RNA-> Protein, is known as the Central Dogma of Molecular Biology. This hands-on module is designed for 9th through 12th grade students. To adjust the difficulty level of the modules, some aspects can be modified or removed based on the grade. This module includes an instructional slide show, video tutorial, assessment options, and links to additional resources.
Teaching Dna Extractions From Fruits (Strawberry, Banana, Kiwi, And Watermelon), Amadea Pourier, Dillon Nelson, Wiyaka Pomarleau, Kay Cutler, Madhav P. Nepal
Teaching Dna Extractions From Fruits (Strawberry, Banana, Kiwi, And Watermelon), Amadea Pourier, Dillon Nelson, Wiyaka Pomarleau, Kay Cutler, Madhav P. Nepal
iLEARN Teaching Resources
In this lesson, students will use a simple DNA extraction solution and isopropyl alcohol to extract DNA from strawberries, banana, kiwi, and watermelon. The extraction solution consists of dish detergent, water, and table salt. Grinding or crushing the soft fruit tissue will separate the cells. The dish detergent breaks down the membranes, exposing the DNA to the solution. The salt helps the DNA molecule to stick together from the proteins. The cold alcohol dissolves everything but the DNA, which forms an insoluble layer of the DNA allowing it to be visible.
Growing South Dakota (Fall 2021 / Winter 2022), College Of Agriculture, Food And Environmental Sciences
Growing South Dakota (Fall 2021 / Winter 2022), College Of Agriculture, Food And Environmental Sciences
Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)
[Page] 3 SDSU Launches New Professional Program in Veterinary Medicine
[Page] 7 The 6th Generation
[Page] 9 First-Gen Proud
[Page] 11 Student Success
[Page] 21 Alumni News
[Page] 27 CAFES News and Updates
[Page] 45 Jackrabbits Now and Then: A Current Student and Alumni Q&A
Role Of Host Restriction Factors On Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Replication, Pratik Katwal
Role Of Host Restriction Factors On Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Replication, Pratik Katwal
Electronic Theses and Dissertations
In this study, the role of IFITM3 on PRRSV replication was studied in vitro by expressing exogenous IFITM3 in MARC-145 cells. An average of 31% reduction in PRRSV N protein expression and an average of 5.4 fold decrease in virus titer in the supernatant were observed in IFITM3 overexpressing cells as compared to vector control cells at 24 hours post infection (hpi). Moreover, there was a positive correlation between interferon- induced IFITM3 up-regulation and reduced PRRSV replication. To determine the role of endogenous IFITM3 in PRRSV replication, siRNA induced knockdown of IFITM3 was employed. RT-PCR validated the successful silencing of …
Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah
Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah
Electronic Theses and Dissertations
Plant-based diets contain numerous flavonoid compounds that produce antibacterial effects and gut health improvement. Genistein is among the most abundant isoflavones present in a plant-based diet and can be found in high amounts in soy products (up to 1g Kg-1). We describe here a robust method to identify genistein tolerant and metabolizing bacteria in swine gut microbiota and to screen the strains that have antibacterial and immunestimulatory properties. Such strains could be developed as non-antibiotic alternatives to prevent enteric infections in pigs and improve gut immunity. To this end, a mini bioreactorbased system was used to enrich genistein metabolizing bacteria …
Decoding The Mystery Of Antibiotic Persistence, Tahmina Hossain
Decoding The Mystery Of Antibiotic Persistence, Tahmina Hossain
Electronic Theses and Dissertations
This work combines microbiology, molecular biology, Next-Generation Sequencing and system biology approaches to explore the mechanism of antibiotic persistence: a multi-drug tolerant, non-dividing, and metabolically altered state present in a subpopulation of cells due to phenotypic diversity rather than genetic variation (i.e. mutations). Persister can survive lethal antibiotic state and resuscitate after the treatment period is over. They are considered as the major contributing factor behind recurring infections. They also have a high mutation rate, which increases the chances of bacteria gaining antibiotic resistance. The formation of this phenotypic variant (persister) threatens the therapeutic effectiveness of antibiotics and understanding how …