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Understanding Human Gut Bacterial Interactions And Their Role In Colonization Resistance Against Clostridioides Difficile Using Mini-Bioreactor Array And Caenorhabditis Elegans As Model Systems, Achuthan Ambat Jan 2023

Understanding Human Gut Bacterial Interactions And Their Role In Colonization Resistance Against Clostridioides Difficile Using Mini-Bioreactor Array And Caenorhabditis Elegans As Model Systems, Achuthan Ambat

Electronic Theses and Dissertations

Although fecal transplantation has shown to be effective against Clostridioides difficile infection (CDI), key species responsible for colonization resistance still need to be better identified. There needs to be more understanding of the rules governing the development of a healthy microbiome upon transplantation. The bottom-up approach of assembling simple to complex communities in model systems such as bioreactors is one of the ways to approach this problem. Studying synthetic communities with less complexity can yield a system-level understanding of microbial interactions. In the first study, we assembled a synthetic community of 14 bacterial species belonging to the core human microbiota …


Rumen-Protected Methionine Supplementation Improves Lactation, Oxidative Status, And Immune Response During A Subclinical Mastitis Challenge In Lactating Dairy Cows., Anita Paz Lopez Jan 2023

Rumen-Protected Methionine Supplementation Improves Lactation, Oxidative Status, And Immune Response During A Subclinical Mastitis Challenge In Lactating Dairy Cows., Anita Paz Lopez

Electronic Theses and Dissertations

Mastitis is a significant problem in the dairy industry, posing numerous challenges for dairy farmers worldwide. Mastitis refers to the inflammation of the mammary gland, primarily caused by bacterial infections. This condition affects the health and well-being of dairy cows and has severe economic implications for farmers and overall dairy production. The prevalence of mastitis substantially threatens milk quality, productivity, and profitability, making it a top concern in the dairy industry. Preventing mastitis in cows is crucial for maintaining the herd's health and ensuring highquality milk production. Nutritional strategies have recently gained greater attention due to a direct impact on …


Decoding The Mystery Of Antibiotic Persistence, Tahmina Hossain Jan 2022

Decoding The Mystery Of Antibiotic Persistence, Tahmina Hossain

Electronic Theses and Dissertations

This work combines microbiology, molecular biology, Next-Generation Sequencing and system biology approaches to explore the mechanism of antibiotic persistence: a multi-drug tolerant, non-dividing, and metabolically altered state present in a subpopulation of cells due to phenotypic diversity rather than genetic variation (i.e. mutations). Persister can survive lethal antibiotic state and resuscitate after the treatment period is over. They are considered as the major contributing factor behind recurring infections. They also have a high mutation rate, which increases the chances of bacteria gaining antibiotic resistance. The formation of this phenotypic variant (persister) threatens the therapeutic effectiveness of antibiotics and understanding how …


Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah Jan 2022

Genistein-Enriched Pig Gut Microbiota Library As A Potential Probiotic Consortium, Theresah Amponsah

Electronic Theses and Dissertations

Plant-based diets contain numerous flavonoid compounds that produce antibacterial effects and gut health improvement. Genistein is among the most abundant isoflavones present in a plant-based diet and can be found in high amounts in soy products (up to 1g Kg-1). We describe here a robust method to identify genistein tolerant and metabolizing bacteria in swine gut microbiota and to screen the strains that have antibacterial and immunestimulatory properties. Such strains could be developed as non-antibiotic alternatives to prevent enteric infections in pigs and improve gut immunity. To this end, a mini bioreactorbased system was used to enrich genistein metabolizing bacteria …


Role Of Host Restriction Factors On Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Replication, Pratik Katwal Jan 2022

Role Of Host Restriction Factors On Porcine Reproductive And Respiratory Syndrome Virus (Prrsv) Replication, Pratik Katwal

Electronic Theses and Dissertations

In this study, the role of IFITM3 on PRRSV replication was studied in vitro by expressing exogenous IFITM3 in MARC-145 cells. An average of 31% reduction in PRRSV N protein expression and an average of 5.4 fold decrease in virus titer in the supernatant were observed in IFITM3 overexpressing cells as compared to vector control cells at 24 hours post infection (hpi). Moreover, there was a positive correlation between interferon- induced IFITM3 up-regulation and reduced PRRSV replication. To determine the role of endogenous IFITM3 in PRRSV replication, siRNA induced knockdown of IFITM3 was employed. RT-PCR validated the successful silencing of …


Assessing Dairy Farm Employees Health, Leyby Stephany Guifarro Rodriguez Jan 2021

Assessing Dairy Farm Employees Health, Leyby Stephany Guifarro Rodriguez

Electronic Theses and Dissertations

This Master thesis evaluates the impact of general health care and eating habits (Study 1), vision care (Study 2), and mental health status (Study 3) on Latino dairy farmworkers lifestyle and work performance in South Dakota. We hypothesized that the health status of dairy workers has a negative impact on the workers’ job performance. In study 1, data from a total of 70 participants were collected, using a face-to-face survey in Spanish, which allowed us to assess various topics and details related to employees’ daily routine tasks, eating habits, and general health status. Furthermore, recommendations to improve general health care …


Cortisol In Hair As A Measure Of Chronic Stress During Sow Gestation And The Pattern Of Cortisol In Blood During Parturition In Sows, Talia Everding Jan 2021

Cortisol In Hair As A Measure Of Chronic Stress During Sow Gestation And The Pattern Of Cortisol In Blood During Parturition In Sows, Talia Everding

Electronic Theses and Dissertations

Cortisol is known as the stress hormone, as it influences many metabolic processes to maintain glucose homeostasis during stressful experiences, including physical and psychological stress. It can be detected in biological matrices such as blood and hair and is released rapidly during sudden stressors and continuously during longterm stress. Blood cortisol fluctuates rapidly in response to acute stressors like pain, exertion, and fear; in hair cortisol accumulates steadily over the period of hair growth and may be useful for detecting chronically elevated cortisol resulting from long-term stress. The objective of this research was to, 1) determine the influence of a …


Characterization Of Cucurbitacin-Inspired Estrone Analogues As Novel Inhibitors Of Human Atp- Binding Cassette Proteins (Abcb1 And Abcc1), Jennifer Kyeremateng Jan 2021

Characterization Of Cucurbitacin-Inspired Estrone Analogues As Novel Inhibitors Of Human Atp- Binding Cassette Proteins (Abcb1 And Abcc1), Jennifer Kyeremateng

Electronic Theses and Dissertations

ATP-binding cassette (ABC) transporters are a large class of integral membrane proteins that contribute to key physiological functions in all organisms by utilizing ATP binding and hydrolysis to transport diverse substrates across membrane barriers. P-glycoprotein (P-gp/ ABCB1) and Multidrug Resistance protein 1 (MRP1/ABCC1) are widely reported ABC transporters associated with multidrug resistance in cancer. Multidrug resistance (MDR) mediated by P-gp and MRP1 is responsible for treatment failures of many metastatic cancers as a result of reduced accumulation, bioavailability and diminished potency of anticancer drugs. Currently, known P-gp and MRP1 inhibitors are limited due to toxicity, lack of selectivity and low …


Profiling Transcription And Retrotransposition Of Mouse L1 Subfamilies, Lingqi Kong Jan 2020

Profiling Transcription And Retrotransposition Of Mouse L1 Subfamilies, Lingqi Kong

Electronic Theses and Dissertations

Transposable elements, also called jumping genes, comprise almost 45% of the human genome. In contrast, only 1% of the human genome is protein-coding sequences. The function and advantages of maintaining such massive copies of transposable elements in the human genome are still unclear. Long interspersed element 1 (L1), the most substantial group and the only active autonomous transposable element in the human genome, has revealed its unique roles in many diseases. The insertional mutagenesis induced by L1 retrotransposition events could threaten human genomic stability and generate unexpected mutations. L1 overexpression has been documented in both somatic and germline cells and, …


The Relationship Between Food Rules And Childhood Obesity In Rural And Non-Rural Families, Anna Venjohn Jan 2020

The Relationship Between Food Rules And Childhood Obesity In Rural And Non-Rural Families, Anna Venjohn

Electronic Theses and Dissertations

Background: Childhood obesity is continuing to rise, leading to long-term health consequences. Research shows that rural populations have higher rates of childhood obesity. There is a lack of research on how the home environment may affect this health disparity. Parents often enforce food rules to control their child’s eating habits, but the difference between the rural and non-rural populations in enforcing these rules is unknown.
Purpose: The purpose of the present study was to determine if there is a significant difference in food rules between rural and non-rural school-aged children, and if these differences correlate to BMI categories.
Methods: Secondary …


Parenting Styles, Parenting Practices, And Dietary Intakes Of Preschoolers And Their Parents, Biyi Chen Jan 2020

Parenting Styles, Parenting Practices, And Dietary Intakes Of Preschoolers And Their Parents, Biyi Chen

Electronic Theses and Dissertations

Emerging research efforts have focused on the role of parents in the development of dietary behaviors of their children. Parental influences play a particularly important role in determining the children’s weight and shaping children’s dietary behaviors, especially during early childhood. Parent-related determinants can be classified as two types—general parenting (parenting style) and specific parenting practices (e.g, food parenting practices). Examining the interactions between parental influences and preschoolers’ and their parental dietary behaviors and may reveal important insights into how parents influence children’s dietary behaviors. Therefore, the goal of this research is to examine the associations between parenting styles, food parenting …


Impact Of Coaching By Extension Professionals On Rural Wellness Coalition Success, Taylor Willhite Jan 2020

Impact Of Coaching By Extension Professionals On Rural Wellness Coalition Success, Taylor Willhite

Electronic Theses and Dissertations

Objective: To quantitatively assess coaches perceived confidence in coaching rural wellness coalitions and to qualitatively determine barriers and facilitators to success in coaching rural wellness coalitions.
Design: A mixed methods design was utilized with quantitative (scorecard) and qualitative (key informant interviews) methodologies.
Setting: Six rural South Dakota wellness coalitions.
Participants: Extension wellness coalition coaches (n=7), one previous and six current.
Intervention: A component of the broader SDSU Extension 1416 Centers for Disease Control (CDC) and Supplemental Nutrition Assistance Program Education (SNAP-Ed) intervention.
Analysis: STATA was utilized for the quantitative scorecard data by running paired ttests. NVivo was utilized in the …


Alternative Treatment Options For Controlling Anthelmintic-Resistant Haemonchus Contortus Populations In South Dakota Sheep Herds, Adam Sarah Jan 2019

Alternative Treatment Options For Controlling Anthelmintic-Resistant Haemonchus Contortus Populations In South Dakota Sheep Herds, Adam Sarah

Electronic Theses and Dissertations

Haemonchus contortus is a common economically important gastrointestinal nematode that obliges the survival and productivity of small ruminants, especially sheep and goats. Anthelmintic resistance is a primary challenge in ruminant health management programs in preventing and controlling the H. contortus populations; therefore, there is a great necessity in finding and developing natural plant products for use as alternatives to commercial anthelmintics for controlling H. contortus in pastured sheep and goats. This dissertation has three major aims conducted in three separate studies: the first in vivo one was to evaluate the extended effect of a springtime, combined-treatment with 3 anthelmintics having …


Performance Differences Between Two Groups Of Commercial Pigs Following Experimental Infection With Prrs Virus 1-7-4, Erin Little Jan 2019

Performance Differences Between Two Groups Of Commercial Pigs Following Experimental Infection With Prrs Virus 1-7-4, Erin Little

Electronic Theses and Dissertations

Porcine reproductive and respiratory syndrome virus (PRRSV) is the costliest swine disease in North America. Vaccines and management alone have not been effective in controlling this disease. Genetic selection for resilience may be a complimentary approach for controlling PRRSV. The objective of this study was to estimate performance differences between two groups of pigs from the same commercial line following infection with PRRSV 1-7-4: 1) pigs sired by boars selected based on a standard index (TN-S), which emphasized feed efficiency and carcass quality; and 2) pigs sired by boars selected based on an experimental index (TN-E), which emphasized feed intake, …


Physical Activity Environments In Rural Communities: Exploring The Relationship Between Community Perceptions And The Environment, Elizabeth M. Bye Jan 2019

Physical Activity Environments In Rural Communities: Exploring The Relationship Between Community Perceptions And The Environment, Elizabeth M. Bye

Electronic Theses and Dissertations

Purpose: To assess the physical activity environment, community perceptions of the physical activity environment, and the relationship between these variables in rural and/or underserved communities with high obesity prevalence.
Methods: The Rural Active Living Assessment (RALA) was used to assess the programs and policies (PPA), town-wide amenities (TWA), and street segments (SSA) of the physical activity environment and the Rural Active Living Perceived Environmental Support Scale (RALPESS) was used to assess community perception of the physical activity environment within eleven rural and/or underserved communities. Each section of the RALA and RALPESS are further broken down into additional subsections in order …


Development And Evaluation Of Vaccine Candidates For Senecavirus A, Bishwas Sharma Jan 2019

Development And Evaluation Of Vaccine Candidates For Senecavirus A, Bishwas Sharma

Electronic Theses and Dissertations

Senecavirus A (SVA) has been associated with several outbreaks of vesicular disease (VD) since 2014 in major swine-producing countries around the world. The virus causes VD that is clinically indistinguishable from foot-and-mouth disease (FMD) in pigs. Currently there are no commercial vaccines available for SVA. Here, we developed a live attenuated SVA strain by mutating a wild type SVA strain SD15-26 (wt SVA). The live attenuated virus, rSVA mSacII, replicated less efficiently in cell cultures in vitro and expressed lower amounts of VP1 and VP2 capsid proteins than wt SVA. Most importantly, the rSVA mSacII virus failed to induce clinical …


Level Of Satisfaction Among Food Pantry Clients, Staff/Volunteers, And Directors And Its Association With Client Choice In Food Pantry Layouts, Myranda Dunmire Jan 2019

Level Of Satisfaction Among Food Pantry Clients, Staff/Volunteers, And Directors And Its Association With Client Choice In Food Pantry Layouts, Myranda Dunmire

Electronic Theses and Dissertations

Objectives: Food insecurity is an issue in rural communities, particularly among food pantry clients. Food pantries may not provide clients with the opportunity to choose their own foods (client choice), and instead may just give a set amount and type of foods. Client choice is important in regards to client health status as well as perceptions of autonomy and quality of life. Although client choice can be promoted in food pantries, it may be difficult to grasp the process of food pantry transitioning and how well changes such as these may be perceived by clients or food pantry personnel. We …


Wellness Policy Awareness Among School Leaders And The Impact On Written Policy Scores, Mariah Reil Jan 2019

Wellness Policy Awareness Among School Leaders And The Impact On Written Policy Scores, Mariah Reil

Electronic Theses and Dissertations

Background: All schools participating in the National School Lunch Program must possess a written School Wellness Policy (SWP) as mandated by the Healthy, Hunger- Free Kids Act of 2010.1 School officials and leaders play a major role in SWP implementation. It is essential to written SWP implementation that school level officials and leaders in the district are aware of the SWP. However, the association between school leader awareness of SWP and policy quality has not yet been studied.
Purpose: The purpose of the present study was to determine if school leader awareness of a SWP impacts the written SWP quality. …


Using Maximum Power As A Variable For 1rm Prediction In The Squat And Bench Press, Sean O'Connor Jan 2019

Using Maximum Power As A Variable For 1rm Prediction In The Squat And Bench Press, Sean O'Connor

Electronic Theses and Dissertations

Many Strength and Conditioning (S&C) coaches utilize a one-rep max (1RM) exercise test to gauge the maximal strength of athletes, and then prescribe resistance training programs based on a relative percentage of 1RM to obtain strength or power adaptations. However, many S&C coaches have raised questions regarding the safety and necessity of a 1RM test. Attempts to mitigate the weaknesses of 1RM testing have led to other methods of testing including repetition max testing (3RM, 5RM, 10RM, etc.) as well as load/velocity profiling. The main purpose of this study is to determine if 1RM can be accurately estimated from maximal …


Novel Hybrid Analogs Of Estrone Origin Exhibits Cytotoxic Effects Against Egfr-Dependent Cancers, Felix Acheampong Jan 2019

Novel Hybrid Analogs Of Estrone Origin Exhibits Cytotoxic Effects Against Egfr-Dependent Cancers, Felix Acheampong

Electronic Theses and Dissertations

Cancer is second only to cardiovascular illnesses as the deadliest human disease globally. Currently, non-small cell lung cancer (NSCLC) and triple negative breast carcinoma (TNBC) are the most frequent types of cancer and have the highest mortalities. Epidermal growth factor receptor (EGFR), a central regulator of tumor progression, is frequently overexpressed in both cancers and is a key clinical target for therapeutic intervention. Natural products and their synthetic analogs have been utilized as EGFR tyrosine kinase inhibitors (TKIs) with potent antitumor effects. However, acquired resistance limits the long-term efficacy of these drugs. Estrone has been used as a scaffold in …


Impact Of Concussion And Orthopedic Injuries On Physical Activity And Quality Of Life After Sport Retirement, Makenna Hancock Jan 2019

Impact Of Concussion And Orthopedic Injuries On Physical Activity And Quality Of Life After Sport Retirement, Makenna Hancock

Electronic Theses and Dissertations

CONTEXT: Concussions and orthopedic injuries represent a significant risk related to participation in collegiate athletics. These injuries can create significant long-term impairments and functional limitations which may also decrease former athletes’ quality of life (QoL) and inhibit their ability to engage in physical activity. OBJECTIVE: To investigate QoL and physical activity levels of former collegiate athletes with a history of concussion and orthopedic injury, orthopedic injury only, and healthy controls. DESIGN: Descriptive, experimental study.
SETTING: Daily life as former National Collegiate Athletic Association Division I collegiate athlete.
PARTICIPANTS: Former Division I collegiate athletes competing in football, women’s soccer, baseball, softball, …


Bovine Cellular And Innate Immune Response To Hemostatic Polysaccharides, Brian Kobylkevich Jan 2019

Bovine Cellular And Innate Immune Response To Hemostatic Polysaccharides, Brian Kobylkevich

Electronic Theses and Dissertations

Chronic wounds are longstanding dermal wounds that do not heal in a normal amount of time and can commonly last for over a year. These wounds plague humans and farm animals alike. Wounds are characterized by chronic inflammation and are often painful, difficult to heal, have a high recurrence rate, and are potentially debilitating. Long term wounds that do not fully heal are distressing for both humans and animals, but understandably more resources have been directed towards humans. Some treatment options for humans may be suitable for adaptation into the field of animal care. Chronic lesions such as bovine digital …


Surveillance Of South Dakota Mosquito Abundance, Infection Rate, And Insecticide Susceptibility, Geoffrey P. Vincent Jan 2018

Surveillance Of South Dakota Mosquito Abundance, Infection Rate, And Insecticide Susceptibility, Geoffrey P. Vincent

Electronic Theses and Dissertations

The aim of this dissertation was to survey and evaluate the abundance, infection rate, and insecticide susceptibility of mosquito fauna present in South Dakota. Mosquito surveillance has been conducted across South Dakota to record and track potential West Nile virus (WNV) vectors from 2004 to 2017. The nuisance mosquito Aedes vexans was found to be the most abundant species overall in the state and most abundant in many of the regions. The WNV vector, Culex tarsalis, was found to be the second most abundant mosquito and the most abundant vector mosquito across the state. However, geospatial variation did exist between …


Universal Swine Influenza Antigen And Vaccine, Zhao Wang Jan 2018

Universal Swine Influenza Antigen And Vaccine, Zhao Wang

Electronic Theses and Dissertations

Swine influenza A viruses pose a serious concern for global health and worldwide swine industry considering their evasion from vaccine-induced immune responses. Formulation of effective influenza vaccines can be complicated by both antigenic shift to a different subtype of hemagglutinin (HAs) and antigenic drift within a particular HA subtype. To address these concerns, we employed a systematic approach to determine the antigenic determinants of swine influenza A viruses by focusing on H1 and H3 subtypes. In parallel, we used a cross-disciplinary approach including structural biology and modeling, virology, and immunology to identify swine influenza A virus-derived HA fusion intermediates broadly …


Differences In Walking Mechanics Between A Traditional Walker And The Kb Balance Trainer, Silvia Zanini Jan 2018

Differences In Walking Mechanics Between A Traditional Walker And The Kb Balance Trainer, Silvia Zanini

Electronic Theses and Dissertations

INTRODUCTION: Millions of individuals with ambulatory difficulties rely on walking aids to maintain independence and mobility. However, users of traditional walkers typically exhibit increased forward lean of their trunk while using the assistive device. The KB Balance Trainer is a new posterior walker designed to facilitate a more erect position during gait. PURPOSE: To compare gait mechanics across three walking conditions: unassisted, using a traditional walker, and using the KB Balance Trainer. METHODS: Seven adults with experience using walkers due to ambulatory difficulties participated in the study. The study consisted of one training session and one gait analysis session. The …


Weight Management Related Factors And Healthy Behaviors In Adults, Sumadhuri Pamarthi Jan 2018

Weight Management Related Factors And Healthy Behaviors In Adults, Sumadhuri Pamarthi

Electronic Theses and Dissertations

Obesity is an ongoing global pandemic that affects more than one in three adults in the United States. Being overweight or obese also increases the risk of chronic health conditions. Weight management does not only center on immediate weight loss but also on the proper maintenance of an appropriate body weight over the years. Methods of weight management include eating a healthy diet and meeting physical activity guidelines. Such weight management practices are important to ensure that the people suffering from obesity can decrease risks of morbidity or even mortality. Nevertheless, unhealthy weight management strategies might lead to unintended health …


Effects Of Parent Role Modeling Behaviors In Correlation To Child Overweight And Obesity In Preschool-Aged Children, Nicole Van Heek Jan 2018

Effects Of Parent Role Modeling Behaviors In Correlation To Child Overweight And Obesity In Preschool-Aged Children, Nicole Van Heek

Electronic Theses and Dissertations

OBJECTIVE: Parents have been proven to shape their children’s health through their growing years.1-6 Extensive research has been done on the correlation between adolescent’s health status and parent’s role modeling behaviors. However, little research has been done in preschool-aged children. Therefore, the purpose of this study was to determine if parenting style with food and meal behaviors was associated with body mass index (BMI) and weight status in preschool-aged children (3-5 years).
METHODS: This study is a part of South Dakota State University’s iGrow Readers study. For this study, a total of 229 child/parent dyads participated from 14 recruited daycare …


Evaluation Of Berberine As An Alternative To Antibiotics In Nursery Pig Diets, Emily Scholtz Jan 2018

Evaluation Of Berberine As An Alternative To Antibiotics In Nursery Pig Diets, Emily Scholtz

Electronic Theses and Dissertations

Pig weaning process results in reduced growth performance and gut health of weaned pigs. Antibiotics can be added in diets for weaned pigs to improve growth performance and gut health, but their use is being discouraged because they can lead to development of antimicrobial resistant microorganisms. Thus, there is need for alternatives to antibiotics in diets for weaned pigs. The overall goal of this thesis research was to determine the effects of plant extracts (berberine, quercetin, and allyl isothiocyante [AITC]) as alternatives to antibiotics in weaned pig diets. The first objective of the research was to determine in vitro antimicrobial …


The Role Of Programs And Policies In Shaping The Observed School Nutrition Environment And Rural Childhood Obesity, Shadai Martin Jan 2018

The Role Of Programs And Policies In Shaping The Observed School Nutrition Environment And Rural Childhood Obesity, Shadai Martin

Electronic Theses and Dissertations

Childhood obesity is an ongoing public health concern that impacts many children in the United States. Research has shown that the prevalence of childhood obesity is higher in rural populations compared to non-rural populations. This may be attributable to differences in the school nutrition environment, particularly school meals and school wellness policy implementation. In order to design effective intervention targeted at weight management and obesity reduction, information regarding the school nutrition environment must be known. Therefore, the purpose of this research was to examine the school nutrition environment, particularly school lunch participation and weight outcomes between metro, non-metro and rural …


Effects Of Enzymatically Hydrolyzed Yeast Supplementation And Supplementation Frequency On Immune Parameters, Performance, And Digestibility Among Periparturient Beef Cows And Calves, Janine Swartz Jan 2018

Effects Of Enzymatically Hydrolyzed Yeast Supplementation And Supplementation Frequency On Immune Parameters, Performance, And Digestibility Among Periparturient Beef Cows And Calves, Janine Swartz

Electronic Theses and Dissertations

Effects of enzymatically hydrolyzed yeast (EHY) and supplementation frequency (FREQ) on immune parameters among beef cows and calves was evaluated. Eighty multiparous (parity = 4.2 ± 0.3) cows were fed a common brome hay-based diet (CP = 8.0 ± 0.17%). Cows were blocked by expected calving date and stratified by body condition score (BCS) before random assignment of treatment. Beginning 88 ± 5 d prior to parturition, cows were provided 1 kg daily or 3 kg every 3 d of a soybean hull-based supplement (CP = 34.0, % of DM) that contained 0 or 3 g/kg EHY. The daily supplement …