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Evidence For Transfer Of Membranes From Mesenchymal Stem Cells To Hl-1 Cardiac Cells, Robert Boomsma, David Geenen
Evidence For Transfer Of Membranes From Mesenchymal Stem Cells To Hl-1 Cardiac Cells, Robert Boomsma, David Geenen
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This study examined the interaction of mouse bone marrow mesenchymal stem cells (MSC) with cardiac HL-1 cells during coculture by fluorescent dye labeling and then flow cytometry. MSC were layered onto confluent HL-1 cell cultures in a 1 : 4 ratio. MSC gained gap junction permeant calcein from HL-1 cells after 4 hours which was partially reduced by oleamide. After 20 hours, 99% MSC gained calcein, unaffected by oleamide. Double-labeling HL-1 cells with calcein and the membrane dye DiO resulted in transfer of both calcein and DiO to MSC. When HL-1 cells were labeled with calcein and MSC with DiO, …