Life Sciences Commons^™

A Review Of Baobab (Adansonia Digitata) Products: Effect Of Processing Techniques, Medicinal Properties And Uses, Donatien Kabore, Hagrétou Sawadogo-Lingani, Bréhima Diawara, Clarise Compaoré, Mamoudou H. Dicko Prof., Mogens Jacobsen

Pr. Mamoudou H. DICKO, PhD

A general literature review including the effect of processing techniques, medicinal value and uses of baobab tree is reported in this manuscript. Baobab tree has multi-purpose uses, as it produces food and non-food products such as medicines, fuel, timber, fodder. Every part of the baobab tree is reported to be useful. The seeds, leaves, roots, flowers, fruit pulp and bark of baobab are edible. Baobab leaves are used in the preparation of soup. Seeds are used as a thickening agent in soups, but they can be fermented and used as a flavouring agent or roasted and eaten as snacks. The …

Identifying Characteristics Of Effective Small Group Learning Valued By Medical Students And Facilitators, Diana T. Robillard, Laura M. Spring, Susan J. Pasquale, Judith A. Savageau

Judith A. Savageau

Background: Small group teaching is an important part of undergraduate medical education, providing the ideal setting for learners to clarify misunderstandings, test hypotheses and evaluate ideas. Many schools undergoing curriculum reform have increased the time students spend in small group learning. However, there is an overall paucity of literature examining case-based small group sessions in medical school.

Objective: This study was designed to examine student and facilitator perceptions of effective case-based small group teaching in the pre-clinical years and compare results in order to identify similarities and differences and identify key areas of disconnect so that the small …

Improving Prenatal Education In A Health Center: A Pilot Study, Marcy Keddy Boucher, Hugh Silk, Judith A. Savageau, Katharine C. Barnard, Mary Flynn

Judith A. Savageau

Background: Currently, the timing and variety of health education topics that are covered during prenatal visits are decided upon by the individual physician caring for the patient. Consequently, some patients do not gain exposure to important subject matter that could potentially improve their satisfaction and pregnancy outcomes. Previously reported studies have found evidence that improved patient education may increase rates of breastfeeding (as well as duration), seat belt use during pregnancy, and decrease preterm low birth weight infants. In addition, one study has shown that when prenatal visits are targeted to specific objectives, the number of prenatal visits can …

Map Kinases Couple Hindbrain-Derived Catecholamine Signals To Hypothalamic Adrenocortical Control Mechanisms During Glycemia-Related Challenges, Arshad M. Khan, Kimberly L. Kaminski, Graciela Sanchez-Watts, Todd A. Ponzio, J. Brent Kuzmiski, Jaideep S. Bains, Alan G. Watts

Arshad M. Khan, Ph.D.

No abstract provided.

Site-Directed Mutations In The C-Terminal Extension Of Human Αb-Crystallin Affect Chaperone Function And Block Amyloid Fibril Formation, T. M. Treweek, Heath Ecroyd, D. M. Williams, S. Meehan, J. A. Carver, M. J. Walker

Heath Ecroyd

Alzheimer’s, Parkinson’s and Creutzfeldt-Jakob disease are associated with inappropriate protein deposition and ordered amyloid fibril assembly. Molecular chaperones, including αBcrystallin, play a role in the prevention of protein deposition. A series of site-directed mutants of the human molecular chaperone, αB-crystallin, were constructed which focused on the flexible C-terminal extension of the protein. We investigated the structural role of this region as well as its role in the chaperone function of αB-crystallin under different types of protein aggregation, i.e. disordered amorphous aggregation and ordered amyloid fibril assembly. It was found that mutation of lysine and glutamic acid residues in the C-terminal …

Hallucination Proneness, Schizotypy And Meta-Cognition, John Stirling, Emma Barkus, Shon Lewis

Emma Barkus

Disordered or maladaptive meta-cognitive processing appears to be a prominent feature for some individuals with a diagnosis of schizophrenia. We sought to establish whether healthy individuals distinguished either in terms hallucination proneness (HP) or level of schizotypy could also be differentiated on the sub-scales of the Meta-cognitions Questionnaire (MCQ), or a modified version of it in which items about worry were replaced with items specifically related to thinking. A total of 106 healthy volunteers completed the Oxford and Liverpool Inventory of Feelings and Experiences and Launay-Slade hallucination scale, the Schizotypal Personality Questionnaire and two versions of the MCQ: the original …

Ecstasy (Mdma) And Its Relationship With Self Report Depression, Anxiety And Schizotypy, Lisa Woods, Emma Barkus

Emma Barkus

Abstract. Aims: The relationship between ecstasy and affective psychological symptoms are still subject to debate. We aimed to examine the relationship between Ecstasy use and self-report depression, anxiety and schizotypy. Design: Data were collected via structured remote self-report questionnaires. Participants: Participants were split into three groups: a control group with no ecstasy use, a light ecstasy user group (<50 >times), and a heavy ecstasy user group (>50 times). Measurements: Amount and patterns of ecstasy use were recorded, the Beck Depression Inventory (BDI), and Beck Anxiety Inventory (BAI) measured depression and anxiety respectively, and schizotypal traits were measured using the …

Cannabis-Induced Psychosis-Like Experiences Are Associated With High Schizotypy, Emma Barkus, John Stirling, Richard Hopkins, Shon Lewis

Emma Barkus

OBJECTIVE: Recent studies have suggested that cannabis use is a risk factor for developing schizophrenia. We tested the hypothesis that cannabis use increases the likelihood of psychosis-like experiences in non-clinical participants who scored highly on a measure of schizotypy. METHOD: The psychological effects of cannabis were assessed in 137 healthy individuals (76% female, mean age 22 years) using a newly developed questionnaire concerned with subjective experiences of the drug: the Cannabis Experiences Questionnaire. The questionnaire has three subscales: Pleasurable Experiences, Psychosis-Like Experiences and After-Effects. Respondents also completed the brief Schizotypal Personality Questionnaire. RESULTS: Cannabis use was reported by 72% of …

Cannabis-Induced Psychotic-Like Experiences Are Predicted By High Schizotypy. Confirmation Of Preliminary Results In A Large Cohort., John Stirling, Emma Barkus, L Nabosi, S Irshad, G Roemer, B Schreudergoidheijt, Shon Lewis

Emma Barkus

Background: Cannabis use has been identified as a possible risk factor for developing schizophrenia. In a previous paper we reported preliminary evidence that cannabis use increases the likelihood of psychosis-like experiences in non-clinical respondents who scored highly on a measure of schizotypy. We now present findings from pooled data from 3 new follow-up studies comprising a sample of 477 respondents, of whom 332 reported using cannabis at least once. Sampling and Methods: As in our previous study, the psychological effects of cannabis were assessed with the Cannabis Experiences Questionnaire, from which 3 subscales can be derived; encompassing pleasurable experiences, psychosis-like …

Suggestibility, Dissociation And Positive Schizotypy, Emma Barkus, John Stirling, John Cavill

Emma Barkus

Abstract. 94 participants, drawn from a parent sample of 1206 individuals who had previously taken part in an assessment of the factor structure of the Oxford-Liverpool Inventory of Feelings and Experiences (O-LIFE), were designated into groups differentiated in terms of low, moderate or high levels of positive schizotypy. All also completed the dissociative experiences scale (DES) and the Inventory of Suggestibility (IS). Our findings suggest that higher reported levels of dissociative experiences and higher suggestibility both independently predict higher scores of positive schizotypy, although suggestibility appears to be a stronger predictor than dissociation.

Schizotypy And Psychosis-Like Experiences From Recreational Cannabis In A Non-Clinical Sample, Emma Barkus, Shon Lewis

Emma Barkus

Background. The relationship between cannabis use and psychosis is still a matter for debate. Accounting for the individual differences in subjective experiences to recreational cannabis use in the general population may hold some clues to the aetiological relationship between cannabis and psychotic symptoms. We hypothesized that schizotypy would account for the individual differences in subjective experiences after cannabis use but not in patterns of use. Method. In a sample of 532 young people who had used cannabis at least once, we examined the relationship between the Cannabis Experiences Questionnaire (CEQ) and the Schizotypal Personality Questionnaire (SPQ). Additionally, we examined the …

Distress And Metacognition In Psychosis Prone Individuals: Comparing High Schizotypes To The At-Risk Mental State, Emma Barkus, John Stirling, Paul French, Anthony Morrison, Richard Bentall, Shon Lewis

Emma Barkus

Both schizotypy and at-risk mental states (ARMS: prodromal states) define individuals at risk for psychotic symptoms. However, the relationship between the 2 is unclear. ARMS individuals are, by definition, help-seeking and therefore at greater risk. We tested whether high schizotypes and ARMS exist along the same continuum by examining maladaptive metacognitions and distress. About 95 healthy volunteers (39% male; mean age, 22.8 years) completed the Schizotypal Personality Questionnaire, the Launay-Slade Hallucinations Scale, Metacognitions Questionnaire (MCQ), and the General Health Questionnaire, and 58 help seeking individuals with ARMS status (41% male; mean age, 22.2 years) completed the Metacognitions Questionnaire and General …

Personality And Cannabis Use, Emma Barkus

Emma Barkus

Cannabis is one of the most widely used illegal substances in the world. Its use has been reported to be over-represented in many psychiatric conditions and has frequently been found to predate the onset of psychiatric symptoms. However, cannabis may also have detrimental effects on the general population. Factors that predict the onset of use are receiving increased attention to aid in identifying groups of young people who may be more prone to consume cannabis. Personality traits may be one such factor as they are readily identifiable and offer information that can be used for improved targeting of educational material …

Midwifery And The Context Of Care: Is It Possible To Be 'Woman Centred'?, Moira Williamson, Lindsey Harrison

Moira Williamson

Title: Midwifery and the context of care: Is it impossible to be ‘woman centred’? Dr Moira Williamson, School of Nursing, Midwifery and Indigenous Health, University of Wollongong, Northfields Avenue, Wollongong NSW 2522, Australia, ph. +61 2 4221 3381, email: moiraw@uow.edu.au Dr Lindsey Harrison, School of Health Sciences, University of Wollongong Background: Findings from a qualitative study show that the context of care can inhibit midwives’ provision of woman centred care; especially impacted is their ability to provide culturally appropriate care. Aim of the Study: The aims of the study were to gain an understanding of midwives’ concepts of culture and …

Workforce Issues, Skill Mix, Maternity Services And The Enrolled Nurse : A Discussion, Moira Williamson, Avon Strahle

Moira Williamson

New South Wales (NSW) is experiencing a shortage of registered midwives. Currently midwives are being actively recruited for 125 metropolitan and 45 rural positions in area health services across the state. This shortage of registered midwives is occurring not only in NSW but also throughout Australia, and has also been reported internationally (McKenna & Hasson, 2002; Keeney et al. 2005). In an attempt to address the ongoing shortage of registered nurses and midwives the NSW Health Department has proposed that a skill mix of 80% registered nurses or midwives to 20% enrolled nurses be implemented within hospitals. This initiative will …

Child Birth: Don't Forget The Fathers, Moira Williamson

Moira Williamson

Fathers are often forgotten when a new baby comes into a family. But men face many challenges in the early months of fatherhood as they come to terms with their new or expanded role. And they need more support. UOW Senior Lecturer and Master of Science (Midwifery) Co-ordinator Moira Williamson and colleagues Professor Carol McVeigh from Massey University (NZ) and Mercy Baafi, Midwifery Manager (Education) at Wollongong Hospital, researched the impact of parenthood with 128 new fathers. MOIRA WILLIAMSON prepared this Opinion column, based on their research.

Phaseolus Beans: Impact On Glycaemic Response And Chronic Disease Risk In Human Subjects, Andrea M. Hutchins, Donna M. Winham, Sharon V. Thompson

Donna Winham

Consumption of Phaseolus vulgaris bean species such as pinto, black, navy or kidney may be beneficial in the prevention and treatment of chronic diseases. In particular, conditions that are promoted by increased glycaemic stress (hyperglycaemia and hyperinsulinaemia) including diabetes, CVD and cancer seem to be reduced in individuals who eat more of these beans. The present paper discusses the influence of P. vulgaris species on glycaemic response and the impact that relationship may have on the risk of developing diabetes, CVD and cancer.

Associations Of Daily Eating Episodes, And Eating Away-From-Home With Blood Level Of Total Cholesterol, Yunsheng Ma, Elizabeth R. Bertone-Johnson, Edward J. Stanek, Dr. Nancy L. Cohen, Ira S. Ockene

Nancy L. Cohen

The objective of this investigation is to describe the associations of number of eating episodes and proportion of meals eaten away from home with total serum cholesterol. Data from 499 participants, recruited from a health maintenance organization in central Massachusetts, aged 20-70, were used for this analysis. Dietary information and total blood cholesterol were obtained at five sampling points (baseline and four consecutive quarters) during the one-year follow-up. A cross-sectional study was conducted. The results from the study do not support the hypothesis that the number of eating episodes per day is associated with total blood cholesterol. However, we noted …

Structural Basis For Coevolution Of A Human Immunodeficiency Virus Type 1 Nucleocapsid-P1 Cleavage Site With A V82a Drug-Resistant Mutation In Viral Protease, Moses Prabu-Jeyabalan, Ellen A. Nalivaika, Nancy M. King, Celia A. Schiffer

Celia A. Schiffer

Maturation of human immunodeficiency virus (HIV) depends on the processing of Gag and Pol polyproteins by the viral protease, making this enzyme a prime target for anti-HIV therapy. Among the protease substrates, the nucleocapsid-p1 (NC-p1) sequence is the least homologous, and its cleavage is the rate-determining step in viral maturation. In the other substrates of HIV-1 protease, P1 is usually either a hydrophobic or an aromatic residue, and P2 is usually a branched residue. NC-p1, however, contains Asn at P1 and Ala at P2. In response to the V82A drug-resistant protease mutation, the P2 alanine of NC-p1 mutates to valine …

Mechanism Of Substrate Recognition By Drug-Resistant Human Immunodeficiency Virus Type 1 Protease Variants Revealed By A Novel Structural Intermediate, Moses Prabu-Jeyabalan, Ellen A. Nalivaika, Keith Romano, Celia A. Schiffer

Celia A. Schiffer

Human immunodeficiency virus type 1 (HIV-1) protease processes and cleaves the Gag and Gag-Pol polyproteins, allowing viral maturation, and therefore is an important target for antiviral therapy. Ligand binding occurs when the flaps open, allowing access to the active site. This flexibility in flap geometry makes trapping and crystallizing structural intermediates in substrate binding challenging. In this study, we report two crystal structures of two HIV-1 protease variants bound with their corresponding nucleocapsid-p1 variant. One of the flaps in each of these structures exhibits an unusual "intermediate" conformation. Analysis of the flap-intermediate and flap-closed crystal structures reveals that the intermonomer …

Viability Of A Drug-Resistant Human Immunodeficiency Virus Type 1 Protease Variant: Structural Insights For Better Antiviral Therapy, Moses Prabu-Jeyabalan, Ellen A. Nalivaika, Nancy M. King, Celia A. Schiffer

Celia A. Schiffer

Under the selective pressure of protease inhibitor therapy, patients infected with human immunodeficiency virus (HIV) often develop drug-resistant HIV strains. One of the first drug-resistant mutations to arise in the protease, particularly in patients receiving indinavir or ritonavir treatment, is V82A, which compromises the binding of these and other inhibitors but allows the virus to remain viable. To probe this drug resistance, we solved the crystal structures of three natural substrates and two commercial drugs in complex with an inactive drug-resistant mutant (D25N/V82A) HIV-1 protease. Through structural analysis and comparison of the protein-ligand interactions, we found that Val82 interacts more …

Role Of Invariant Thr80 In Human Immunodeficiency Virus Type 1 Protease Structure, Function, And Viral Infectivity, Jennifer E. Foulkes-Murzycki, Moses Prabu-Jeyabalan, Deyna Cooper, Gavin J. Henderson, Janera Harris, Ronald I. Swanstrom, Celia A. Schiffer

Celia A. Schiffer

Sequence variability associated with human immunodeficiency virus type 1 (HIV-1) is useful for inferring structural and/or functional constraints at specific residues within the viral protease. Positions that are invariant even in the presence of drug selection define critically important residues for protease function. While the importance of conserved active-site residues is easily understood, the role of other invariant residues is not. This work focuses on invariant Thr80 at the apex of the P1 loop of HIV-1, HIV-2, and simian immunodeficiency virus protease. In a previous study, we postulated, on the basis of a molecular dynamics simulation of the unliganded protease, …

Perceptions Of Flatulence From Bean Consumption Among Adults In 3 Feeding Studies, Donna M. Winham, Andrea M. Hutchins

Donna Winham

Background: Many consumers avoid eating beans because they believe legume consumption will cause excessive intestinal gas or flatulence. An increasing body of research and the 2010 Dietary Guidelines for Americans supports the benefits of a plant-based diet, and legumes specifically, in the reduction of chronic disease risks. The purpose of the current research was to investigate the perception of increased flatulence and gastrointestinal discomfort among participants who consumed a ½ cup of beans daily for 8 or 12 weeks. Methods: Participants in three studies to test the effects of beans on heart disease biomarkers completed the same weekly questionnaire to …

Introducing Functional Data Analysis To Neuroimaging, And Vice Versa, Philip T. Reiss

Philip T. Reiss

No abstract provided.

Lipopolysaccharide Biosynthesis Without The Lipids: Substrate Recognition For Escherichia Coli Heptosyltransferasei, Daniel J. Czyzyk, Cassie Liu, Erika A. Taylor

Erika A. Taylor, Ph.D.

Heptosyltransferase I (HepI) is responsible for the transfer of l-glycero-d-manno-heptose to a 3-deoxy-α-D-oct-2-ulopyranosonic acid (Kdo) of the growing core region of lipopolysaccharide (LPS). The catalytic efficiency of HepI with the fully deacylated analogue of Escherichia coli HepI LipidA is 12-fold greater than with the fully acylated substrate, with a k(cat)/K(m) of 2.7 × 10(6) M(-1) s(-1), compared to a value of 2.2 × 10(5) M(-1) s(-1) for the Kdo(2)-LipidA substrate. Not only is this is the first demonstration that an LPS biosynthetic enzyme is catalytically enhanced by the absence of lipids, this result has significant implications for downstream enzymes that …

Front Of Pack Daily Intake Labelling On Australian Packaged Foods: Introduction And Use 2007-2009, P Williams, R Duncan, K De Agnoli, A Hull, A Owers, T Wang

Peter Williams

This study aimed to measure the extent of use of front-of-pack daily intake (DI) labelling across food categories in Australian supermarkets, and assess the level of compliance with the Australian Food and Grocery Council (AFGC) guidelines. Surveys of six supermarkets in the Illawarra region of New South Wales were conducted twice a year in 2007, 2008 and 2009. The number of products with DI labelling increased from 58 in February 2007 to 1939 in August 2009 and appears to be growing strongly. The greatest number of products with the labelling are in the biscuits and crackers, cooking sauces, breakfast cereals, …

Breast Cancer Detection Messages In Australian Print Media Advertising - Are They Promoting Correct Information?, Sandra C. Jones, John R. Rossiter

Sandra Jones

With breast cancer now the leading cause of cancer deaths among women in the western world, correct information about detection and treatment is vitally important. The media are often accused of conveying inaccurate information in their editorial coverage of health issues, but few studies have examined the accuracy of information in media advertising. In this study of breast cancer detection ads in Australian magazines and newspapers, many instances of misleading information were found. As the print media have a major influence on women's health beliefs, these findings have serious implications for health communication policy regarding socially responsible advertising.

Disease Awareness Advertising: Women's Intentions Following Exposure, Danika Hall, Sandra C. Jones, Donald C. Iverson

Sandra Jones

Background: In Australia, where direct to consumer advertising of prescription medicines is prohibited, pharmaceutical companies can sponsor disease awareness advertising targeting consumers. This study examined the impact of disease awareness advertising exposure on older women's reported behavioural intentions. Method: Women were approached in a shopping centre and randomly assigned mock advertisements for two health conditions. Disease information and sponsors were manipulated. Results: Two hundred and forty-one women responded to 466 advertisements. Almost half reported an intention to ask their doctor for a prescription or referral as a result of seeing the advertisement, but more reported they would talk to their …

Alcohol Energy Drinks: Is It Time For The Alcohol Industry To Demonstrate Some Corporate Social Responsibility?, Sandra Jones

Sandra Jones

There is increasing evidence of association between AEDs and both increased alcohol consumption and increased alcohol-related harm. Australia lags behind other countries in researching and regulating (alcoholic and non-alcoholic) energy drinks. Twelve focus groups were conducted with 12-17 year olds in a capital city, regional city and rural city in NSW, Australia. AEDs were popular among teenagers in urban, regional and rural areas of NSW. Adolescents liked AEDs because they provide increased energy while allowing them to feel the effects of alcohol, and because of their similarity to non-alcohol energy drinks and soft drinks. Given the industry’s stated position on …

Point-Of-Sale Alcohol Promotions In The Perth And Sydney Metropolitan Areas, Sandra C. Jones, Lance R. Barrie

Sandra Jones

There is considerable evidence that reducing the price of alcohol increases the amount consumed, particularly among young people. However, there is an absence of research on other point-of-sale (POS) alcohol marketing strategies - such as those that ‘reward’ purchasers with free gifts. The purpose of the present study was to document the nature and extent of POS alcohol promotions in bottle shops in two Australian capital cities. An audit tool was developed and point-of-sale promotions were found to be ubiquitous, with 416 promotions identified across 24 audits (an average of 17.3 promotions per outlet; 19.8 in Sydney and 14.9 in …