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Full-Text Articles in Life Sciences
Phagosomal Signaling By Borrelia Burgdorferi In Human Monocytes Involves Toll-Like Receptor (Tlr) 2 And Tlr8 Cooperativity And Tlr8-Mediated Induction Of Ifn-Β, Jorge L. Cervantes, Star M. Dunham-Ems, Carson J. La Vake, Mary M. Petzke, Justin D. Radolf, Juan C. Salazar
Phagosomal Signaling By Borrelia Burgdorferi In Human Monocytes Involves Toll-Like Receptor (Tlr) 2 And Tlr8 Cooperativity And Tlr8-Mediated Induction Of Ifn-Β, Jorge L. Cervantes, Star M. Dunham-Ems, Carson J. La Vake, Mary M. Petzke, Justin D. Radolf, Juan C. Salazar
UCHC Articles - Research
Phagocytosed Borrelia burgdorferi (Bb) induces inflammatory signals that differ both quantitatively and qualitatively from those generated by spirochetal lipoproteins interacting with Toll-like receptor (TLR) 1/2 on the surface of human monocytes. Of particular significance, and in contrast to lipoproteins, internalized spirochetes induce transcription of IFN-β. Using inhibitory immunoregulatory DNA sequences (IRSs) specific to TLR7, TLR8, and TLR9, we show that the TLR8 inhibitor IRS957 significantly diminishes production of TNF-α, IL-6, and IL-10 and completely abrogates transcription of IFN-β in Bb-stimulated monocytes. We demonstrate that live Bb induces transcription of TLR2 and TLR8, whereas IRS957 interferes with their transcriptional regulation. Using …