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Generation And Characterization Of Col10a1 -Mcherry Reporter Mice, Peter Maye, Yu Fu, Yaling Liu, Mary Louise Stover, Xi Jiang, David W. Rowe
Generation And Characterization Of Col10a1 -Mcherry Reporter Mice, Peter Maye, Yu Fu, Yaling Liu, Mary Louise Stover, Xi Jiang, David W. Rowe
UCHC Articles - Research
We report here on the generation of a new fluorescent protein reporter transgenic mouse line, Col10a1 -mCherry, which can be used as a tool to study chondrocyte biology and pathology. Collagen, Type X, alpha 1(Col10a1) is highly expressed in hypertrophic chondrocytes and commonly used as a gene marker for this cell population. TheCol10a1-mCherry reporter line was generated using a bacterial recombination strategy with the mouse BAC clone RP23-192A7. To aid in the characterization of this animal model, we intercrossed Col10a1-mCherry mice with Collagen, Type II, alpha 1 (Col2a1) enhanced cyan fluorescent protein (ECFP) reporter mice and characterized the expression of …