Life Sciences Commons^™

Vitro Bacteriophage Treatment For A Staphylococcus Aureus And Pseudomonas Aeruginosa Co-Culture, Alexis Perry

Arkansas Women in STEM Conference

Recent studies show that up 27-36% of all infections are polymicrobial, involving more than one bacteria species. In all categories, polymicrobial infections are often more virulent and harmful to the patient. Since different types of bacteria can communicate with each other through quorum-sensing when in the presence of each other, they can display different genomic characteristics. Further research into polymicrobial infections needs to be done because these changing characteristics can prevent treatment from working, such as antibiotics or bacteriophage therapy. This experiment focused on the co-culture between the two bacteria, Pseudomonas aeruginosa and Staphylococcus aureus, which can be found co-existing …

Assessing The Reliability, Internal Consistency, And Sensitivity Of A Nutrition Knowledge Questionnaire For Four-Year-Old Pre-K Children, Lin Wang, Karla Cortez, Brenda Rodriguez, Joseph Reyes, Moises Cisneros, Elizabeth Alanis, Zasha Romero, Lisa Michelle Belzer Salinas, Juan López Alvarenga, Roberto Treviño-Peña

Research Symposium

Introduction: Assessing nutrition knowledge in four-year-olds, a developmental stage marked by limited attention spans and varying comprehension abilities, is challenging with traditional methods. A reliable test is pivotal for establishing a foundation for future health interventions. We evaluated a 13-question nutrition and healthy habits test in Pre-K4 children to assess reliability and sensitivity to detect differences.

Methods: Calculations included Cronbach's alpha, kappa coefficient, McNemar analysis by item, and Bland-Altman plots for test-retest differences. Mixed model regression assessed the questionnaire's sensitivity by sex and association with age. Item response theory (IRT) models were employed, generating latent abilities for students and individual …

Determining The Ideal Concentrations Of Ethanol And Propylene Glycol In Ethosomes For Transdermal Delivery Of Vitamin D3, Rebecca Conner

Harrisburg University Research Symposium: Highlighting Research, Innovation, & Creativity

Vitamin D₃ is an important chemical in the human body, however, many Americans have low levels of this vitamin. There are plenty of oral supplementations for Vitamin D₃ deficiency, but those of older age and busy schedules may struggle to meet the minimum requirement. A recently developed ethosomal transmembrane delivery system (Touitou, 2000), similar to liposomes but also containing ethanol, allows users to apply a gel dermally and have the desired drug or active ingredient reach the bloodstream faster. However, there is considerable variation in the concentration of ethanol and permeation enhancers used. Using an affordable method to …

Core Activation Through The Measurement Of Traditional Vs. Non-Traditional Core Exercises, Erica Stilwell, Taylor Moran, Chris Couch

Scholars Day Conference

Athletic trainers at the collegiate level have a responsibility to their athletes to prepare them in core endurance and strength for their specific needs. The purpose of this study was to compare the effectiveness of muscle fiber recruitment between traditional core exercises and non-traditional core exercises measured by an electromyography (EMG) machine in different vital core muscles. Twenty-four college students participated and were randomly sorted into two exercise groups, traditional or non-traditional. The traditional core exercise group performed ten bicycle crunches while the non-traditional core exercise group performed ten stability ball passes for each trial recorded. The participants wore four …

Nutrition Knowledge Of Young Adults: How Has K-12 Education Affected The Health Of Young Adults In 2023, Anna Polo

Scholars Day Conference

Background: Nutrition knowledge is very important in order to live a healthy lifestyle and to positively impact overall health. There is research studying the effects of nutrition education and that affects students involved in K-12 schooling, but there is a lack of research following those students beyond the classroom and to their young adult years.

Objective: The researcher’s goal was to better understand how much young adults know about basic nutrition from previous education, if different types of schooling (public, private, homeschool, etc.) taught nutrition related classes/lessons, and if different geographical regions provided nutrition education.

Design: the design was a …

University Students' Understanding Of The Endocannabinoid System And Its Relevance To Nutrition, Brittney Burford, Allyson Melton, Robbie Otero

Scholars Day Conference

The Endocannabinoid System (ECS) is a complex cell-signaling system that was identified in 1988, the ECS is the largest receptor system and regulator of homeostasis. Endogenous cannabinoids, or endocannabinoids, are naturally occurring, lipid-based neurotransmitters. Neurotransmitters are chemical messengers in the body that send signals between nerve cells. The ECS system regulates and controls many of our most critical bodily functions such as learning, memory, emotional processing, sleep, temperature control, inflammatory immune responses, and appetite. Tetrahydrocannabinol (THC) and cannabidiol (CBD) are the most known phytocannabinoids. Phytocannabinoids are plant byproducts that closely mimic cannabinoids and have adverse effects on the body. Endogenous …

Fighting Malnutrition In Kenya, Jessica Payton, Tara Ross, Adalyne Nehus

ATU Research Symposium

No abstract provided.

Evaluation Of Concussion Assessment Tools For Collegiate Athletes, Jacqueline D. Villanueva Arevalo, Emily R. Brown

Student Scholar Showcase

BACKGROUND: Due to the increase in sports-related concussions in the United States, there is a rising concern about concussion assessments in collegiate athletes with regard to long term vestibular ocular motor dysfunction and post-concussion syndrome.

PURPOSE: Due to varying practices in concussion diagnosis, this systematic review analyzed three concussion diagnostic instruments, The King Devick, Screening Concussion Assessment Tool (SCAT-5), and Vestibular Oculomotor Screening Tool (VOMS), to determine the most appropriate instrument(s) for reducing vestibular ocular-motor dysfunction and post-concussion symptoms in collegiate athletes.

METHODS: Nested Knowledge was used to identify articles from PubMed, Europe Pubmed Central, and ClinicalTrials.gov databases. Key search …

Functional Analysis Provides Insight Into Missing Heritability, Scott L. Baughan, Michael A. Tainsky, Fatima Darwiche

Medical Student Research Symposium

Accurate ascertainment of genetic risk can be potentially lifesaving for patients who inherit cancer promoting mutations. However, even with the most extensive panel testing clinically available, a large number of patients will test negative despite family history of cancer or test positive for a variant of unknown significance (VUS). For these patients, clinical management is complicated; patients want to know their risk, and may fear disease they are not at great risk for (benign VUS) or they may not be given access to potentially lifesaving early screening procedures (pathogenic VUS). ATM has proven a challenge to clinicians due to its …

Investigating Respiratory Simplification Caused By Prenatal Rage Upregulation, Paul Reynolds

Annual Research Symposium

No abstract provided.

Potential Role For Rage In The Development Of Secondhand Smoke-Induced Chronic Sinusitis, Paul Reynolds

Annual Research Symposium

No abstract provided.

Rage Inhibition Reduces Timp And Decreases Cell Invasion In Cigarette Smoke Extract (Cse) Treated Oral Squamous Carcinoma Cells, Paul Reynolds

Annual Research Symposium

No abstract provided.

Rage Availability Lessens The Degree Of Dysfunctional Mitochondrial Bioenergetics Following Chronic Secondhand Smoke Exposure, Paul Reynolds

Annual Research Symposium

No abstract provided.

Gestational Dependent Responses To Second Hand Smoke (Shs) In Mouse Pregnancies, Paul Reynolds

Annual Research Symposium

No abstract provided.

Prevention Of Doxorubicin-Induced Cardiotoxicity By A Coumestan Analogue, Psoralidin, Mujtaba H. Shah, Myia Aiges, Kota V. Ramana

Annual Research Symposium

Natural phenolic compound Psoralidin limits cardiotoxicity associated with Doxorubicin

Science Alive, Trisha Sudhakar, Rajin Persaud

Trick to the Treat of Internships and Research

The goal of Science Alive is to provide a “sense of wonder” not only for the grade-school children but for the college students and their faculty as well. Science Alive serves to reinforce the scientific knowledge of local grade-school students and community members, while enhancing the education and career development opportunities for college students. Our group does this by providing opportunities for youth to explore science beyond the textbooks through hands-on, interactive discovery, which stimulates interest and fosters confidence for college and grade-school students alike. Our groups lead on-campus and in-class workshops, weekend clubs, after-school programs, and other community events. …

Sea-Phages Research Opportunity, Sarah Ballarin

Trick to the Treat of Internships and Research

Research opportunity targeted for freshmen and sophomores through the SEA-PHAGES program. The goal of the research is to discover new bacteriophages (viruses that infect bacteria) to help combat antibiotic resistance. Phage therapy is a promising alternative to conventional antibiotics. Phages have also been widely used in the food industry to prevent microbial growth on certain foods and are currently being explored as a method for bioremediation of oil spills and wastewater treatment.

Last year, my research team discovered two new bacteriophages: Genamy16 and NovaSharks that were able to infect the bacteria Gordonia rubripertincta. These phages can possibly be used for …

Trick To The Treat: Penn Medicine Princeton Health Hospital, Divya Papaiya

Trick to the Treat of Internships and Research

No abstract provided.

Pfizer Summer Growth Experience, Arman Kakkar

Trick to the Treat of Internships and Research

From May 9, 2022 till July 29, 2022, I served as a Research Intern at Pfizer. Specifically, I worked in the Vaccine Research and Development Sector in the High Throughput Clinical Immunoassays and Diagnostics Group (HCID). In this group, I was responsible for Phase 3 testing of clinical trial material for the Covid-19 Vaccine. Over this time, I learned a great deal about the process of vaccine production from start to finish. Additionally, I was able to make numerous connections. This is definitely an experience I would recommend to others.

The Use Of Molecular Beacon Sirna To Induce Degradation Of Sars Cov-2 Mrna In Infected Cells, Katelynn Sepke, Hanna Dutt

Liberty University Research Week

Undergraduate

Basic

Casein Kinases Interact And Stabilize Glucose Sensing Receptors In Yeast, Julia Humble, Levi Mailloux, Sophia Adams

Liberty University Research Week

Undergraduate

Basic

Binding Affinities Of Bpa Derivatives To The Human Estrogen Receptor: Utilization And Assessment Of Autodock Vina, Luke Newman

Liberty University Research Week

Undergraduate

Basic

Investigating Alternative Induction Of The S. Aureus 80a Prophage, Jody Caretti

Student Scholar Showcase

Widespread antibiotic resistance has quickly become one of the most concerning crises affecting modern medicine, especially in cases of Staphylococcal infections, which have become resistant to all ��-lactam antibiotics. As a result, research into alternative forms of treatment for bacterial infections is a top priority. Bacteriophage therapy is a popular candidate for replacing antibiotics, due to the way the viruses interact with the bacteria. The 80�� prophage integrates its DNA into Staphylococcus aureus strain 10616 via the lysogenic cycle, and replicates with the bacterial cell. When stressed, the bacteria stop replicating and the prophage stimulates the lytic cycle, activating transcription …

Ingredient Classification Using Food Ontology, Ricky Flores

UNO Student Research and Creative Activity Fair

A food label provides some of the most crucial information for a food product. The food label is a key resource for many health-conscious consumers for understanding ingredients. It is also vital for individuals to avoid food allergens or help patients follow dietary recommendations. While the food labels in the United States are regulated by the Food and Drug Administration (FDA) many labels contain additional information or statements that are not regulated. Moreover, the food label may be complex or contain terminology that the layperson may not understand. Evidence has indicated that consumers often find nutrition labels confusing, especially when …

Seizure Prediction In Epilepsy Patients, Gary Dean Cravens

NSU REACH and IPE Day

Purpose/Objective: Characterize rigorously the preictal period in epilepsy patients to improve the development of seizure prediction techniques. Background/Rationale: 30% of epilepsy patients are not well-controlled on medications and would benefit immensely from reliable seizure prediction. Methods/Methodology: Computational model consisting of in-silico Hodgkin-Huxley neurons arranged in a small-world topology using the Watts-Strogatz algorithm is used to generate synthetic electrocorticographic (ECoG) signals. ECoG data from 18 epilepsy patients is used to validate the model. Unsupervised machine learning is used with both patient and synthetic data to identify potential electrophysiologic biomarkers of the preictal period. Results/Findings: The model has shown states corresponding to …

Phage Hunters: The Discovery Of Two Novel Bacteriophages That Infect Gordonia Rubripertincta, Sarah Ballarin, Neel Balusa, Melissa Bell, Samia Caballero, Joshua Chan, Maria Farez, Ashley J. Guillen-Tapia, Nashrah Pierre-Louis, Victoria Polishuk, Bhavya Soni, Julie Torruellas Garcia, Katie E. Crump

Sea-Phages

Antibiotic resistance is a global healthcare concern because it poses a threat to effectively treating bacterial infections and the need for alternative therapeutics are urgently required. Phage therapy, which uses viruses to treat bacterial infections, is a promising alternative to conventional antibiotics. Phages have also been widely used in the food industry to prevent microbial growth on certain foods and are currently being explored as a method for bioremediation of oil spills and wastewater treatment. Although phages are ubiquitous in nature, many have yet to be discovered. Through the SEA-PHAGES (Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science) program …

Effect Of Carbon Monoxide Releasing Molecule 3 (Corm - 3) On Platelet Adhesion To Human Brain Microvascular Endothelial Cells, Najat S. El-Farra

Undergraduate Student Research Internships Conference

Sepsis is characterized by the widespread inflammation of the body. Systemic inflammation activates and recruits inflammatory cells (e.g., leukocytes) and platelets to the affected organs.

During these inflammatory conditions, human brain microvascular endothelial cells (hBMEC) and platelets both upregulate adhesive molecules rendering platelets to adhere to hBMEC.

Although carbon monoxide is thought of as a toxic molecule to many, previous work shows its anti-inflammatory properties. Evidence has shown carbon monoxide-releasing molecules (e.g., CORM-3; that release small, non-toxic amounts of CO) can combat the effects of severe inflammation in several in vivo animal model.

In this current study, we are looking …

Efficacy Of Curcumin In Asthma: A Systematic Review And Meta Analysis, Tabitha Grow, Adam Sadowski

McNair Symposium

Over 23 million people are affected by asthma in the United States and 262 million individuals globally. Asthma, if poorly controlled, is associated with significant morbidity as well as increased risk for mortality. Asthma involves several complex inflammatory pathways and processes including an increase in immune cell activation. Curcumin, the active constituent found in turmeric, has been studied in numerous in-vivo and in-vitro studies to generate anti-inflammatory effects in pulmonary diseases and, more recently, an increase in clinical data has become available. Since the most recent review assessing the role of curcumin in pulmonary disorders, additional clinical trials have been …

Stream Sediment Monitoring In Morehead Kentucky, Kristopher Krolikowski, Jen O'Keefe

2021 Celebration of Student Scholarship - Oral Presentations

Sediment loads in stream systems play a big role in how overall runoff behaves. Streams naturally transport sediment at various ratios depending on precipitation. When vegetation is removed in upstream environments, sediment loads on streams increase. Sudden increases of sediments in streams can cause many issues, such as flooding or stream re-direction. In Morehead, KY, large-scale logging operations on the ridge above Knapp Avenue and on the ridge above the north end of Allen Avenue have caused increased sediment load in a small stream called Oxley Branch. This stream flows south parallel to Knapp Avenue and along the eastern property …

Analysis Of Botulinum Toxin A And Interacting Proteins In Skeletal Muscle Cells: An Investigation Into The Mechanisms Of Botulinum Toxin A As A Treatment For Chronic Exertional Compartment Syndrome, Lauren Kee

Pence-Boyce STEM Student Scholarship

Background: Chronic exertional compartment syndrome (CECS) is a condition in which muscle tissue expands against the surrounding fascia during activity and is compressed along with the nerves and blood vessels within the muscle compartment, leading to abnormally high intracompartmental pressure (ICP) and debilitating pain. Treatment typically includes fasciotomy, which results in significant levels of CECS recurrence; however, botulinum toxin A (BoNT-A) injection has recently been seen to decrease both ICP and pain through an unknown mechanism with little to no recurrence.

Methods: In this study, cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), prostaglandin E₂ receptor 4 (EP4), endothelial nitric oxide synthase (eNOS), …