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Full-Text Articles in Life Sciences
Impaired T Cell Proliferation In Acute Dengue Infection, Anuja Mathew, Ichiro Kurane, Sharone Green, David Vaughn, Siripen Kalayanarooj, Saroj Suntayakorn, Francis Ennis, Alan Rothman
Impaired T Cell Proliferation In Acute Dengue Infection, Anuja Mathew, Ichiro Kurane, Sharone Green, David Vaughn, Siripen Kalayanarooj, Saroj Suntayakorn, Francis Ennis, Alan Rothman
Alan Rothman
Decreased proliferative responses to mitogens and recall Ags have been observed in PBMC obtained during several acute human viral infections. To determine whether cell-mediated responses are altered during acute dengue infection, we examined the proliferative responses of PBMC from children enrolled in a prospective study of dengue infections in Thailand. All responses of PBMC during acute illness were compared with the same patients' PBMC obtained at least 6 mo after their infection. Proliferative responses to PHA, anti-CD3, tetanus toxoid, and dengue Ags were decreased significantly in PBMC obtained during the acute infection. The proliferative responses to PHA were restored by …
Transient Decreases In Human T Cell Proliferative Responses Following Vaccinia Immunization, Anuja Mathew, Francis Ennis, Alan Rothman
Transient Decreases In Human T Cell Proliferative Responses Following Vaccinia Immunization, Anuja Mathew, Francis Ennis, Alan Rothman
Alan Rothman
To further study the immunosuppression associated with virus infections, we analyzed the proliferative responses of serial PBMC samples obtained following vaccinia virus immunization. In four of five volunteers, responses to PHA, anti-CD3, vaccinia virus, and recall antigens were markedly decreased at at least one time point between days 5 and 29 after vaccination. Responses to PHA were restored by the addition of IL-2 or irradiated autologous healthy PBMC in the two volunteers tested, suggesting that the proliferation defect is attributable to accessory cell dysfunction. In one donor, immobilized anti-CD3 failed to induce proliferation, but addition of immobilized anti-CD28 partially restored …
Partial Agonist Effect Influences The Ctl Response To A Heterologous Dengue Virus Serotype, Jaroslav Zivny, Matthew Defronzo, William Jarry, Julie Jameson, John Cruz, Francis Ennis, Alan Rothman
Partial Agonist Effect Influences The Ctl Response To A Heterologous Dengue Virus Serotype, Jaroslav Zivny, Matthew Defronzo, William Jarry, Julie Jameson, John Cruz, Francis Ennis, Alan Rothman
Alan Rothman
Activation of dengue serotype-cross-reactive memory CTL during secondary dengue virus (DV) infection is thought to be important in the pathogenesis of dengue hemorrhagic fever. To model this effect, we studied the CTL responses to DV types 2 (D2V) and 3 (D3V) in PBMC from an individual previously infected with D3V. DV-specific CD8+ CTL from this donor recognized two HLA-B62-restricted epitopes on the NS3 protein, aa 71-79 (SVKKDLISY) and 235-243 (AMKGLPIRY). Both D3V-specific and D2V/D3V-cross-reactive CTL clones were detected for each epitope; all D2V-reactive CTL clones could lyse D2V-infected autologous cells. CTL responses to both epitopes were detected in bulk cultures …
Bystander Target Cell Lysis And Cytokine Production By Dengue Virus-Specific Human Cd4(+) Cytotoxic T-Lymphocyte Clones, Susan Gagnon, Francis Ennis, Alan Rothman
Bystander Target Cell Lysis And Cytokine Production By Dengue Virus-Specific Human Cd4(+) Cytotoxic T-Lymphocyte Clones, Susan Gagnon, Francis Ennis, Alan Rothman
Alan Rothman
Dengue hemorrhagic fever, the severe form of dengue virus infection, is believed to be an immunopathological response to a secondary infection with a heterologous serotype of dengue virus. Dengue virus capsid protein-specific CD4(+) cytotoxic T-lymphocyte (CTL) clones were shown to be capable of mediating bystander lysis of non-antigen-presenting target cells. After activation by anti-CD3 or in the presence of unlabeled antigen-presenting target cells, these clones could lyse both Jurkat cells and HepG2 cells as bystander targets. Lysis of HepG2 cells suggests a potential role for CD4(+) CTL in the liver involvement observed during dengue virus infection. Three CD4(+) CTL clones …
Dominant Recognition By Human Cd8+ Cytotoxic T Lymphocytes Of Dengue Virus Nonstructural Proteins Ns3 And Ns1.2a, Anuja Mathew, Ichiro Kurane, Alan Rothman, Lingling Zeng, Margo Brinton, Francis Ennis
Dominant Recognition By Human Cd8+ Cytotoxic T Lymphocytes Of Dengue Virus Nonstructural Proteins Ns3 And Ns1.2a, Anuja Mathew, Ichiro Kurane, Alan Rothman, Lingling Zeng, Margo Brinton, Francis Ennis
Alan Rothman
A severe complication of dengue virus infection, dengue hemorrhagic fever (DHF), is hypothesized to be immunologically mediated and virus-specific cytotoxic T lymphocytes (CTLs) may trigger DHF. It is also likely that dengue virus-specific CTLs are important for recovery from dengue virus infections. There is little available information on the human CD8+ T cell responses to dengue viruses. Memory CD8+CTL responses were analyzed to determine the diversity of the T cell response to dengue virus and to identify immunodominant proteins using PBMC from eight healthy adult volunteers who had received monovalent, live-attenuated candidate vaccines of the four dengue serotypes. All the …
Immunopathologic Mechanisms Of Dengue Hemorrhagic Fever And Dengue Shock Syndrome, Ichiro Kurane, Alan Rothman, Peter Livingston, Sharone Green, Susan Gagnon, Jurand Janus, Bruce Innis, Suchitra Nimmannitya, Ananda Nisalak, Francis Ennis
Immunopathologic Mechanisms Of Dengue Hemorrhagic Fever And Dengue Shock Syndrome, Ichiro Kurane, Alan Rothman, Peter Livingston, Sharone Green, Susan Gagnon, Jurand Janus, Bruce Innis, Suchitra Nimmannitya, Ananda Nisalak, Francis Ennis
Alan Rothman
Dengue virus infections are a major cause of morbidity and mortality in tropical and subtropical areas of the world. The immunopathological mechanisms that result in severe complications of dengue virus infection, i.e. dengue hemorrhagic fever (DHF), are important to determine. Primary dengue virus infections induce serotype-specific and serotype-cross-reactive, CD4+ and CD8+ memory cytotoxic T lymphocytes (CTL). In secondary infections with a virus of a different serotype from that which caused primary infections, the presence of cross-reactive non-neutralizing antibodies results in an increased number of infected monocytes by dengue virus--antibody complexes. This in turn results in marked activation of serotype cross-reactive …