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Total Cellular Atp Production Changes With Primary Substrate In Mcf7 Breast Cancer Cells., Maggie Louie, Justin Ton, Maurice L. Brady, Diem T. Le, Jordon N. Mar, Chad A Lerner, Akos A Gerencser, Shona A. Mookerjee
Total Cellular Atp Production Changes With Primary Substrate In Mcf7 Breast Cancer Cells., Maggie Louie, Justin Ton, Maurice L. Brady, Diem T. Le, Jordon N. Mar, Chad A Lerner, Akos A Gerencser, Shona A. Mookerjee
Natural Sciences and Mathematics | Faculty Scholarship
Cancer growth is predicted to require substantial rates of substrate catabolism and ATP turnover to drive unrestricted biosynthesis and cell growth. While substrate limitation can dramatically alter cell behavior, the effects of substrate limitation on total cellular ATP production rate is poorly understood. Here, we show that MCF7 breast cancer cells, given different combinations of the common cell culture substrates glucose, glutamine, and pyruvate, display ATP production rates 1.6-fold higher than when cells are limited to each individual substrate. This increase occurred mainly through faster oxidative ATP production, with little to no increase in glycolytic ATP production. In comparison, non-transformed …