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Medical Specialties

Selected Works

2012

Urinary bladder

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Adrenoceptor Function And Expression In Bladder Urothelium And Lamina Propria, Christian Moro, Lotti Tajouri, Russ Chess-Williams Nov 2012

Adrenoceptor Function And Expression In Bladder Urothelium And Lamina Propria, Christian Moro, Lotti Tajouri, Russ Chess-Williams

Christian Moro

Objective - To investigate the role of adrenoceptor subtypes in regulating the spontaneous contractile activity of the inner lining of the urinary bladder (urothelium/lamina propria).

Materials and Methods - The responses of isolated strips of porcine urothelium/lamina propria to noradrenaline, phenylephrine, and isoprenaline were obtained in the absence and presence of receptor subtype-selective antagonists. Quantitative reverse-transcriptase polymerase chain reaction was undertaken to assess the expression of adrenoceptor genes.

Results - The tissues expressed all α1- and β-adrenoceptor subtypes, with the α1A-, α1B-, and β2-adrenoceptors the predominant receptors at the messenger RNA level. In the functional experiments, the rate of phasic …

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Non-Adrenergic, Non-Cholinergic, Non-Purinergic Contractions Of The Urothelium/Lamina Propria Of The Pig Bladder, Christian Moro, Russ Chess-Williams Sep 2012

Non-Adrenergic, Non-Cholinergic, Non-Purinergic Contractions Of The Urothelium/Lamina Propria Of The Pig Bladder, Christian Moro, Russ Chess-Williams

Christian Moro

Summary
1. Acetylcholine, and to a lesser extent ATP, mediates neurogenic contractions of bladder smooth muscle. Recently, the urothelium and lamina propria have also been shown to have contractile properties, but the neurotransmitters involved in mediating responses to nerve stimulation have not been investigated.
2. Isolated strips of porcine urothelium with lamina propria were electrically field stimulated and contractions recorded. Drugs interfering with neurotransmission were then employed to identify which neurotransmitters mediated responses.
3. Strips of urothelium/lamina propria developed spontaneous contractions with a frequency of 3.5 ± 0.1 cycles min_1 and amplitude of 0.84 ± 0.06 g. Electrical field stimulation …

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Contractile Activity Of The Bladder Urothelium/Lamina Propria And Its Regulation By Nitric Oxide, Christian Moro, Charlotte Leeds, Russ Chess-Williams Jan 2012

Contractile Activity Of The Bladder Urothelium/Lamina Propria And Its Regulation By Nitric Oxide, Christian Moro, Charlotte Leeds, Russ Chess-Williams

Christian Moro

In the bladder, nitric oxide (NO) is released from neuronal and non-neuronal sources, but its actions are unclear. Strips of urothelium plus lamina propria contract in response to agonists and develop spontaneous phasic contractions, and the aim of this study was to investigate the influence of NO on this activity. Isolated strips of urothelium/lamina propria from porcine bladder developed spontaneous contractions (3.5 +/- 0.3 cycles/min) and contracted in response to carbachol and electrical field stimulation (EFS). The NO synthase inhibitor N-omega-nitro-L-arginine (L-NNA, 100 mu M) had no effects on the tissues, but the NO donors diethylamine NONOate (DEANO, 100 mu …

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