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Biochemical And Immunochemical Comparison Of Africanized And European Honeybee Venoms, D. R. Nelson, Anita M. Collins, Richard L. Hellmich, R. T. Jones, R. M. Helm, D. L. Squillace, J. W. Yunginger
Biochemical And Immunochemical Comparison Of Africanized And European Honeybee Venoms, D. R. Nelson, Anita M. Collins, Richard L. Hellmich, R. T. Jones, R. M. Helm, D. L. Squillace, J. W. Yunginger
Richard L Hellmich
Africanized honeybees (HBs) pose a hazard to both normal and sting-sensitive subjects in certain areas of Central and South America, and it is predicted that they will soon be present in the southern United States as well. Using an electrical stimulation device, we collected Africanized HB venom (AHV) in Venezuela and European HB venom (EHV) in Louisiana. These venoms, along with commercial European HB venom (CHV), were compared by thin-layer isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The Coomassie brilliant blue and silver-stained banding patterns of AHV and EHV were essentially identical to CHV. Western blots were prepared …