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Evaluation Of The Photometric Assay Method To Quantitate The Bactericidal Action Of Normal And Immune Rabbit Sera On Salmonella Pullorum, Alice Margaret Rahn
Evaluation Of The Photometric Assay Method To Quantitate The Bactericidal Action Of Normal And Immune Rabbit Sera On Salmonella Pullorum, Alice Margaret Rahn
Loma Linda University Electronic Theses, Dissertations & Projects
The bactericidal antibody test depends on the presence of sufficient complement, magnesium ions, and specific antibody to produce killing of certain gram negative bacilli. This study was undertaken to demonstrate whether or not Salmonella pullorum is an organism which is susceptible to this killing action.
Different amounts of test serum containing antibody, constant amounts of complement and magnesium saline diluent were placed together and held in a 37°C water bath for a one hour reaction time. During this interval, the antibody-complement system was given an opportunity to kill the susceptible bacteria. Then, a constant volume of nutrient broth was added …