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Urothelial/Lamina Propria Spontaneous Activity And The Role Of M3 Muscarinic Receptors In Mediating Rate Responses To Stretch And Carbachol, Christian Moro, Jumpei Uchiyama, Russ Chess-Williams
Urothelial/Lamina Propria Spontaneous Activity And The Role Of M3 Muscarinic Receptors In Mediating Rate Responses To Stretch And Carbachol, Christian Moro, Jumpei Uchiyama, Russ Chess-Williams
Christian Moro
Objective: To investigate the effects of tissue stretch and muscarinic receptor stimulation on the spontaneous activity of the urothelium/lamina propria and identify the specific receptor subtype mediating these responses.
Methods: Isolated strips of porcine urothelium with lamina propria were set up for in vitro recording of contractile activity. Muscarinic receptor subtype-selective antagonists were used to identify the receptors influencing the contractile rate responses to stretch and stimulation with carbachol.
Results: Isolated strips of urothelium with lamina propria developed spontaneous contractions (3.7 cycles/min) that were unaffected by tetrodotoxin, N_-nitro-L-arginine, or indomethacin. Carbachol (1 _M) increased the spontaneous contractile …