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A Survey Of Proteomic Biomarkers For Heterotopic Ossification In Blood Serum, Laura E. Edsberg, Erin Crowgey, Partrick Osborn, Jennifer Wyffels
A Survey Of Proteomic Biomarkers For Heterotopic Ossification In Blood Serum, Laura E. Edsberg, Erin Crowgey, Partrick Osborn, Jennifer Wyffels
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Background: Heterotopic ossification (HO) is a significant problem for wounded warriors surviving high-energy blast injuries; however, currently, there is no biomarker panel capable of globally characterizing, diagnosing, and monitoring HO progression. The aim of this study was to identify biomarkers for HO using proteomic techniques and blood serum.
Methods: Isobaric tags for relative and absolute quantitation (iTRAQ) was used to generate a semi-quantitative global proteomics survey of serum from patients with and without heterotopic ossification. Leveraging the iTRAQ data, a targeted selection reaction monitoring mass spectrometry (SRM-MS) assay was developed for 10 protein candidates: alkaline phosphatase, osteocalcin, alpha-2 type I …