Life Sciences Commons^™

Evaluation Of Automated Ribotyping As A Tool For Bacterial Source Tracking In Aquatic Environments, Tracy L. Idocks

USF Tampa Graduate Theses and Dissertations

An Escherichia coli ribotype profile library was created for the Tampa Bay watershed for application to bacterial source tracking of human or non-human fecal contamination to the bay and surrounding rivers. Bacterial source tracking uses a variety of methods to identify the source of fecal contamination, not just the quantity of the indicator bacteria present, allowing implementation of effective management practices. The 700 confirmed E. coli isolate library was equally distributed among four sources: human, bird, dog and cow. The library was tested for applicability for source tracking within the Tampa Bay watershed. The DuPont Qualicon RiboPrinter® Microbial Characterization System, …

Assessment Of Current And New Methods For Investigating The Occurrence Of Enteric Pathogens In Ambient Waters And Water Sources For Rehydration Of A Floridan Wetland, Walter Quintero-Betancourt

USF Tampa Graduate Theses and Dissertations

Water quality assessment is currently used for determining the risks from contamination with waterborne pathogenic microorganisms. The public health. significance of such microorganisms cannot be fully understood without the application of reliable environmental monitoring techniques that provide efficient recovery as well as information on the infectious potential of specific isolates. This research was carried out to assess the analytical performance of current and new methods for investigating the occurrence of waterborne Cryptosporidium oocysts in samples of surface water, groundwater and reclaimed water used for public access irrigation in the State of Florida.

Method 1623 of the U.S Environmental Protection Agency …