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Marine Biology

Great Lakes Institute for Environmental Research Publications

Cyanophage

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Full-Text Articles in Life Sciences

Multi-Year Molecular Quantification And ‘Omics Analysis Of Planktothrix-Specific Cyanophage Sequences From Sandusky Bay, Lake Erie, Katelyn M. Mckindles, Makayla Manes, Michelle Neudeck, Robert Michael Mckay, George S. Bullerjahn Jan 2023

Multi-Year Molecular Quantification And ‘Omics Analysis Of Planktothrix-Specific Cyanophage Sequences From Sandusky Bay, Lake Erie, Katelyn M. Mckindles, Makayla Manes, Michelle Neudeck, Robert Michael Mckay, George S. Bullerjahn

Great Lakes Institute for Environmental Research Publications

Introduction: Planktothrix agardhii is a microcystin-producing cyanobacterium found in Sandusky Bay, a shallow and turbid embayment of Lake Erie. Previous work in other systems has indicated that cyanophages are an important natural control factor of harmful algal blooms. Currently, there are few cyanophages that are known to infect P. agardhii, with the best-known being PaV-LD, a tail-less cyanophage isolated from Lake Donghu, China. Presented here is a molecular characterization of Planktothrix specific cyanophages in Sandusky Bay. Methods and Results: Putative Planktothrix-specific viral sequences from metagenomic data from the bay in 2013, 2018, and 2019 were identified by two approaches: homology …


Dissolved Microcystin Release Coincident With Lysis Of A Bloom Dominated By Microcystis Spp. In Western Lake Erie Attributed To A Novel Cyanophage, Katelyn M. Mckindles, Makayla A. Manes, Jonathan R. Demarco, Andrew Mcclure, R. Michael Mckay, Timothy W. Davis, George S. Bullerjahn Nov 2020

Dissolved Microcystin Release Coincident With Lysis Of A Bloom Dominated By Microcystis Spp. In Western Lake Erie Attributed To A Novel Cyanophage, Katelyn M. Mckindles, Makayla A. Manes, Jonathan R. Demarco, Andrew Mcclure, R. Michael Mckay, Timothy W. Davis, George S. Bullerjahn

Great Lakes Institute for Environmental Research Publications

Western Lake Erie (Laurentian Great Lakes) is prone to annual cyano- bacterial harmful algal blooms (cHABs) dominated by Microcystis spp. that often yield microcystin toxin concentrations exceeding the federal EPA recreational con-tact advisory of 8 μg liter-1. In August 2014, microcystin levels were detected in fin-ished drinking water above the World Health Organization 1.0 μg liter-1 threshold for consumption, leading to a 2-day disruption in the supply of drinking water for >400,000 residents of Toledo, Ohio (USA). Subsequent metatranscriptomic analysis of the 2014 bloom event provided evidence that release of toxin into the water sup-ply was likely caused by cyanophage …