Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- EUTROPHICATION (2)
- BAND DISEASE (1)
- BASIN (1)
- BIOMASS (1)
- Ballast water (1)
-
- CARBON LOSS (1)
- Coral; Microbial communities; Host-microbe symbiosis; Holobiont; PNA clamp; 16S rRNA gene sequencing; PEPTIDE NUCLEIC-ACID; CORAL; AMPLIFICATION; RESILIENCE; DIVERGENCE; DIVERSITY; DYNAMICS; TISSUE (1)
- Disease reservoir (1)
- Disease transmission (1)
- Disease vector (1)
- Eutrophication (1)
- FLORIDA (1)
- GEN. NOV. (1)
- Histology16S (1)
- LAND-USE (1)
- LOADS (1)
- LOUISIANA (1)
- MARINE (1)
- MISSISSIPPI RIVER (1)
- MISSOURI RIVER (1)
- Microbial communities (1)
- NITROGEN (1)
- NUTRIENT (1)
- NUTRIENT ENRICHMENT (1)
- Nutrient removal (1)
- ORGANIC-CARBON (1)
- PHYTOPLANKTON (1)
- Restoration (1)
- River diversion (1)
- SILVA (1)
Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Peak Chlorophyll A Concentrations In The Lower Mississippi River From 1997 To 2018, R. Eugene Turner, Charles S. Milan, Erick M. Swenson, James M. Lee
Peak Chlorophyll A Concentrations In The Lower Mississippi River From 1997 To 2018, R. Eugene Turner, Charles S. Milan, Erick M. Swenson, James M. Lee
Faculty Publications
Large and turbid rivers have varying temperatures, light conditions, nutrient availability, and nutrient ratios that may affect phytoplankton communities and occur within a changing world of point and nonpoint source nutrient loadings. We investigated how these physical and chemical factors affect Chlorophyll a (Chl a) concentrations in the Mississippi River, the largest river in North America, by sampling 878 times from February 1997 to December 2018 near its terminus at Baton Rouge, Louisiana. We hypothesized that nutrient concentrations and ratios were significant factors limiting phytoplankton biomass accumulations in this turbid river. The Chl a concentrations were in the "poor" water …
Mass Removal Efficiencies In Water And Consequences After A River Diversion Into Coastal Wetlands: Second Thoughts, R. Eugene Turner, Erik M. Swenson, James M. Lee, Charles S. Milan
Mass Removal Efficiencies In Water And Consequences After A River Diversion Into Coastal Wetlands: Second Thoughts, R. Eugene Turner, Erik M. Swenson, James M. Lee, Charles S. Milan
Faculty Publications
Salinity control, nutrient additions, and sediment supply were directly or indirectly the rationale for a $220 million coastal wetland restoration project (Davis Pond River Diversion) that began in 2002. We sampled Mississippi River water going in and out of the receiving basin from 1999 to 2018 to understand why wetland loss increased after it began. There was a reduction in inorganic sediments, nitrogen (N), and phosphorus (P) concentrations within the ponding area of 77%, 39% and 34%, respectively, which is similar to that in other wetlands. But the average sediment accumulation of 0.6 mm year(-1) inadequately balances the present-day 5.6 …
Reef Sediments Can Act As A Stony Coral Tissue Loss Disease Vector, Michael S. Studivan, Ashley M. Rossin, Ewelina Rubin, Nash Soderberg, Daniel M. Holstein, Ian C. Enochs
Reef Sediments Can Act As A Stony Coral Tissue Loss Disease Vector, Michael S. Studivan, Ashley M. Rossin, Ewelina Rubin, Nash Soderberg, Daniel M. Holstein, Ian C. Enochs
Faculty Publications
Stony coral tissue loss disease (SCTLD) was first observed in 2014 near Virginia Key in Miami-Dade County, Florida. Field sampling, lab experiments, and modeling approaches have suggested that reef sediments may play a role in SCTLD transmission, though a positive link has not been tested experimentally. We conducted an ex situ transmission assay using a statistically-independent disease apparatus to test whether reef sediments can transmit SCTLD in the absence of direct contact between diseased and healthy coral tissue. We evaluated two methods of sediment inoculation: batch inoculation of sediments collected from southeast Florida using whole colonies of diseased Montastraea cavernosa, …
Reducing Host Dna Contamination In 16s Rrna Gene Surveys Of Anthozoan Microbiomes Using Pna Clamps, Alicia M. Reigel, Sarah M. Owens, Michael E. Hellberg
Reducing Host Dna Contamination In 16s Rrna Gene Surveys Of Anthozoan Microbiomes Using Pna Clamps, Alicia M. Reigel, Sarah M. Owens, Michael E. Hellberg
Faculty Publications
Efforts to study the microbial communities associated with corals can be limited by inefficiencies in the sequencing process due to high levels of host amplification by universal bacterial 16S rRNA gene primers. Here, we develop an inexpensive peptide nucleic acid (PNA) clamp that binds to a target sequence of host DNA during PCR and blocks amplification. We then test the ability of this PNA clamp to mitigate host contamination and increase overall microbial sequence coverage on samples from three coral species: the gorgoniansEunicea flexuosaandGorgonia ventalina,and the scleractinianPorites panamensis. The 20-bp PNA clamp was designed using DNA fromE. flexuosa. Adding the …